Tunicamycin 0.04μM R07 exp418 [The Human Chemical-Genetic Interaction Map Project]

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====== Tunicamycin 0.04μM R07 exp418 ======
==== Mechanism of Action ====
Inhibits GlcNAc phosphotransferase (GPT), blocks N-linked glycoslylation, induces protein unfolding in ER, nucleoside antibiotic
  * **<color #00a2e8>Class / Subclass 1:</color>** Proteostasis / Unfolded Protein Response Activator
  * **<color #00a2e8>Class / Subclass 2:</color>** Infectious Disease / Antibiotic



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Tunicamycin from Streptomyces sp.%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 11089-65-9
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/16220051|16220051]]
    * **<color #00a2e8>IUPAC:</color>** %%(E)-N-[(2S,3R,4R,5R,6R)-2-[(2S,3S,4S,5R,6S)-3-acetamido-4,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-[(2R)-2-[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]-2-hydroxyethyl]-4,5-dihydroxyoxan-3-yl]-12-methyltridec-2-enamide%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C37H60N4O16/c1-18(2)12-10-8-6-4-5-7-9-11-13-23(45)39-26-30(50)27(47)21(54-36(26)57-35-25(38-19(3)43)29(49)28(48)22(17-42)55-35)16-20(44)33-31(51)32(52)34(56-33)41-15-14-24(46)40-37(41)53/h11,13-15,18,20-22,25-36,42,44,47-52H,4-10,12,16-17H2,1-3H3,(H,38,43)(H,39,45)(H,40,46,53)/b13-11+/t20-,21-,22+,25+,26-,27+,28+,29+,30-,31+,32-,33-,34-,35+,36+/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** YJQCOFNZVFGCAF-WPTOCQRYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 816.9
    * **<color #00a2e8>Canonical SMILES:</color>** CC(C)CCCCCCCCC=CC(=O)NC1C(C(C(OC1OC2C(C(C(C(O2)CO)O)O)NC(=O)C)CC(C3C(C(C(O3)N4C=CC(=O)NC4=O)O)O)O)O)O
    * **<color #00a2e8>Isomeric SMILES:</color>** CC(C)CCCCCCCC/C=C/C(=O)N[C@@H]1[C@H]([C@H]([C@H](O[C@H]1O[C@H]2[C@H]([C@@H]([C@H]([C@@H](O2)CO)O)O)NC(=O)C)C[C@H]([C@@H]3[C@H]([C@H]([C@@H](O3)N4C=CC(=O)NC4=O)O)O)O)O)O
    * **<color #00a2e8>Molecular Formula:</color>** C37H60N4O16
{{:chemogenomics:structures:chem-0251.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** T7765
    * **<color #00a2e8>Lot #:</color>** 037M4047V
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+NH4)+ Calcd for C37H60N4O16 834.43426; found 834.4337
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Tunicamycin
    * **<color #00a2e8>Min:</color>** -6.5253; **<color #00a2e8>Max:</color>** 80.8037
{{:chemogenomics:dose_response:dr_411.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.0211    |
| IC30  |0.0328    |
| IC40  |0.0492    |
| IC50  |0.0748    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 40nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.0
    * **<color #00a2e8>Numbers of reads</color>**: 19675060


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|4/15|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Tunicamycin_0.04uM_Round-7_exp418.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Tunicamycin 0.04μM R07 exp418'>
{{:chemogenomics:cranks_plots:exp418.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:D:DPAGT1|DPAGT1]]|-4.23|<0.01|
|[[:human_genes:D:DNAJC3|DNAJC3]]|-3.37|<0.01|
|[[:human_genes:R:RNF149|RNF149]]|-2.91|<0.01|
|[[:human_genes:C:CXCR4|CXCR4]]|-2.87|0.01|
|[[:human_genes:A:ASB3|ASB3]]|-2.68|0.06|
|[[:human_genes:N:NOC3L|NOC3L]]|-2.65|0.22|
|[[:human_genes:Z:ZBTB1|ZBTB1]]|-2.57|0.05|
|[[:human_genes:T:TM2D2|TM2D2]]|-2.38|0.11|
|[[:human_genes:S:SEC23B|SEC23B]]|-2.24|0.22|
|[[:human_genes:G:GPR75-ASB3|GPR75-ASB3]]|-2.23|0.22|
|[[:human_genes:M:MBTPS2|MBTPS2]]|-2.21|0.22|
|[[:human_genes:A:ADCYAP1R1|ADCYAP1R1]]|-2.19|0.23|
|[[:human_genes:U:UBE2D3|UBE2D3]]|-2.15|0.22|
|[[:human_genes:C:C19orf25|C19orf25]]|-2.14|0.25|
|[[:human_genes:E:ERGIC3|ERGIC3]]|-2.14|0.22|
|[[:human_genes:S:SLC22A14|SLC22A14]]|-2.14|0.25|
|[[:human_genes:E:EDF1|EDF1]]|-2.13|0.22|
|[[:human_genes:D:DHRSX|DHRSX]]|-2.13|0.22|
|[[:human_genes:S:SEPHS2|SEPHS2]]|-2.12|0.22|
|[[:human_genes:A:ARL1|ARL1]]|-2.11|0.22|
|[[:human_genes:O:OR4F21|OR4F21]]|-2.11|0.49|
|[[:human_genes:S:SEC31A|SEC31A]]|-2.09|0.22|
|[[:human_genes:S:SMCHD1|SMCHD1]]|-2.09|0.22|
|[[:human_genes:Z:ZNF567|ZNF567]]|-2.08|0.22|
|[[:human_genes:G:GATAD2A|GATAD2A]]|-2.06|0.23|
|[[:human_genes:Z:ZMYM2|ZMYM2]]|-2.04|0.24|
|[[:human_genes:Z:ZC3H13|ZC3H13]]|-2.01|0.37|
|[[:human_genes:Q:QRICH1|QRICH1]]|-2.00|0.44|
|[[:human_genes:E:ENY2|ENY2]]|-1.98|0.37|
|[[:human_genes:D:DERL2|DERL2]]|-1.98|0.29|
|[[:human_genes:R:RSBN1L|RSBN1L]]|2.17|0.17|
|[[:human_genes:S:SP2|SP2]]|2.18|0.12|
|[[:human_genes:D:DDX23|DDX23]]|2.20|0.45|
|[[:human_genes:E:ETFB|ETFB]]|2.22|0.15|
|[[:human_genes:S:SURF6|SURF6]]|2.25|0.21|
|[[:human_genes:C:C1orf177|C1orf177]]|2.27|0.13|
|[[:human_genes:P:PSMD1|PSMD1]]|2.31|0.21|
|[[:human_genes:P:PDS5B|PDS5B]]|2.32|0.06|
|[[:human_genes:D:DRG1|DRG1]]|2.33|0.11|
|[[:human_genes:E:ELOVL1|ELOVL1]]|2.34|0.06|
|[[:human_genes:S:SPI1|SPI1]]|2.36|0.10|
|[[:human_genes:T:THRSP|THRSP]]|2.42|0.04|
|[[:human_genes:E:ETFA|ETFA]]|2.42|0.04|
|[[:human_genes:P:PRR14L|PRR14L]]|2.49|0.04|
|[[:human_genes:S:SLC39A9|SLC39A9]]|2.52|0.05|
|[[:human_genes:N:NAA50|NAA50]]|2.58|0.04|
|[[:human_genes:N:NPY4R|NPY4R]]|2.63|0.04|
|[[:human_genes:A:ACACA|ACACA]]|2.67|0.04|
|[[:human_genes:R:RPS9|RPS9]]|2.75|0.21|
|[[:human_genes:E:ELF1|ELF1]]|2.80|<0.01|
|[[:human_genes:S:SCAP|SCAP]]|2.91|<0.01|
|[[:human_genes:R:RPL30|RPL30]]|3.00|0.17|
|[[:human_genes:T:TMEM147|TMEM147]]|3.10|<0.01|
|[[:human_genes:O:OST4|OST4]]|3.14|0.06|
|[[:human_genes:G:GALE|GALE]]|3.18|<0.01|
|[[:human_genes:E:EIF2AK3|EIF2AK3]]|3.47|<0.01|
|[[:human_genes:M:MGAT1|MGAT1]]|3.84|<0.01|
|[[:human_genes:M:MAGT1|MAGT1]]|4.87|<0.01|
|[[:human_genes:S:STT3B|STT3B]]|5.69|<0.01|
|[[:human_genes:M:MFSD2A|MFSD2A]]|7.10|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp419|Tunicamycin 0.04 to 0.075μM  on day4 R07 exp419]]|0.229|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp420|Tunicamycin 0.04 to 0.125μM  on day4 R07 exp420]]|0.203|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp351|Dexamethasone 0.006μM R07 exp351]]|0.055|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp306|Rapamycin 2μM R07 exp306]]|0.051|<button type="primary" size="small" modal="4">View</button>|
</modal>


<modal id="1" size="lg" title="Tunicamycin 0.04 to 0.075μM  on day4 R07 exp419  vs  Tunicamycin 0.04μM R07 exp418">
{{:chemogenomics:comparison_plots:exp418_vs_exp419.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Tunicamycin 0.04 to 0.125μM  on day4 R07 exp420  vs  Tunicamycin 0.04μM R07 exp418">
{{:chemogenomics:comparison_plots:exp418_vs_exp420.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Dexamethasone 0.006μM R07 exp351  vs  Tunicamycin 0.04μM R07 exp418">
{{:chemogenomics:comparison_plots:exp351_vs_exp418.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Rapamycin 2μM R07 exp306  vs  Tunicamycin 0.04μM R07 exp418">
{{:chemogenomics:comparison_plots:exp306_vs_exp418.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|oligosaccharyltransferase complex|127.53|STT3B,MAGT1,OST4|
|protein N-linked glycosylation via asparagine|96.11|STT3B,MAGT1,MGAT1,OST4|
|protein N-linked glycosylation|32.04|STT3B,MAGT1,MGAT1,OST4|
</modal>