VHL-ligand-1 20μM R07 exp421 [The Human Chemical-Genetic Interaction Map Project]

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====== VHL-ligand-1 20μM R07 exp421 ======
==== Mechanism of Action ====
Binds VHL, targeting moiety for PROTACs
  * **<color #00a2e8>Class / Subclass 1:</color>** Proteostasis / Bivalent Ligand




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Protein degrader 1 hydrochloride%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 1448189-80-7
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/118864076|118864076]]
    * **<color #00a2e8>IUPAC:</color>** %%(2S,4R)-1-[(2S)-2-amino-3,3-dimethylbutanoyl]-4-hydroxy-N-[[4-(4-methyl-1,3-thiazol-5-yl)phenyl]methyl]pyrrolidine-2-carboxamide;hydrochloride%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C22H30N4O3S.ClH/c1-13-18(30-12-25-13)15-7-5-14(6-8-15)10-24-20(28)17-9-16(27)11-26(17)21(29)19(23)22(2,3)4;/h5-8,12,16-17,19,27H,9-11,23H2,1-4H3,(H,24,28);1H/t16-,17+,19-;/m1./s1
    * **<color #00a2e8>INCHI Key:</color>** JYRTWGCWUBURGU-MSSRUXLCSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 467
    * **<color #00a2e8>Canonical SMILES:</color>** CC1=C(SC=N1)C2=CC=C(C=C2)CNC(=O)C3CC(CN3C(=O)C(C(C)(C)C)N)O.Cl
    * **<color #00a2e8>Isomeric SMILES:</color>** CC1=C(SC=N1)C2=CC=C(C=C2)CNC(=O)[C@@H]3C[C@H](CN3C(=O)[C@H](C(C)(C)C)N)O.Cl
    * **<color #00a2e8>Molecular Formula:</color>** C22H31ClN4O3S
{{:chemogenomics:structures:chem-0252.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Cayman Chemical
    * **<color #00a2e8>Catalog #:</color>** 21591
    * **<color #00a2e8>Lot #:</color>** 0505579-1
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C22 H30 N4 O3 S 431.21114; found 431.21176
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** VHL_Ligand_1
    * **<color #00a2e8>Min:</color>** -13.9762; **<color #00a2e8>Max:</color>** 12.9391
{{:chemogenomics:dose_response:dr_455.png?nolink&500 |}}
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |N/A    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 20µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 7.0
    * **<color #00a2e8>Numbers of reads</color>**: 18199347


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|0/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/VHL_Ligand_1_20uM_Round-7_exp421.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='VHL-ligand-1 20μM R07 exp421'>
{{:chemogenomics:cranks_plots:exp421.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:A:ASB3|ASB3]]|-2.96|0.06|
|[[:human_genes:Z:ZCRB1|ZCRB1]]|-2.61|0.20|
|[[:human_genes:R:RTCB|RTCB]]|-2.48|0.20|
|[[:human_genes:D:DPAGT1|DPAGT1]]|-2.35|0.20|
|[[:human_genes:F:FAF2|FAF2]]|-2.34|0.24|
|[[:human_genes:Z:ZRANB2|ZRANB2]]|-2.28|0.20|
|[[:human_genes:S:STIM2|STIM2]]|-2.26|0.20|
|[[:human_genes:N:NFS1|NFS1]]|-2.26|0.73|
|[[:human_genes:P:PRAMEF10|PRAMEF10]]|-2.25|0.20|
|[[:human_genes:R:RAB13|RAB13]]|-2.25|0.20|
|[[:human_genes:E:ERCC2|ERCC2]]|-2.18|0.55|
|[[:human_genes:V:VEZT|VEZT]]|-2.17|0.26|
|[[:human_genes:X:XPO5|XPO5]]|-2.11|0.42|
|[[:human_genes:R:RPE|RPE]]|-2.08|0.42|
|[[:human_genes:U:UHRF1|UHRF1]]|-2.08|0.42|
|[[:human_genes:F:FAM193B|FAM193B]]|-2.07|0.41|
|[[:human_genes:G:GAGE2A|GAGE2A]]|-2.06|0.83|
|[[:human_genes:P:PMM2|PMM2]]|-2.06|0.41|
|[[:human_genes:F:FKBP5|FKBP5]]|-2.04|0.42|
|[[:human_genes:H:HNRNPCL2|HNRNPCL2]]|-2.02|0.55|
|[[:human_genes:K:KHDRBS1|KHDRBS1]]|-2.02|0.42|
|[[:human_genes:H:HSPA13|HSPA13]]|-2.02|0.49|
|[[:human_genes:S:SCGB2B2|SCGB2B2]]|-1.98|0.42|
|[[:human_genes:T:TRMT10A|TRMT10A]]|-1.98|0.42|
|[[:human_genes:T:TEN1|TEN1]]|-1.98|0.42|
|[[:human_genes:T:TREML1|TREML1]]|-1.97|0.49|
|[[:human_genes:C:CD68|CD68]]|-1.95|0.69|
|[[:human_genes:M:MYO10|MYO10]]|-1.94|0.46|
|[[:human_genes:G:GPR180|GPR180]]|-1.92|0.55|
|[[:human_genes:T:TPD52L2|TPD52L2]]|-1.90|0.49|
|[[:human_genes:S:SF3A1|SF3A1]]|1.92|0.78|
|[[:human_genes:S:SLC12A1|SLC12A1]]|1.92|0.66|
|[[:human_genes:E:ETFA|ETFA]]|1.94|0.66|
|[[:human_genes:R:RPL30|RPL30]]|1.95|0.85|
|[[:human_genes:S:SYNGR4|SYNGR4]]|1.95|0.66|
|[[:human_genes:C:CETN1|CETN1]]|1.96|0.66|
|[[:human_genes:N:NSUN5|NSUN5]]|1.98|0.66|
|[[:human_genes:K:KIAA1211|KIAA1211]]|1.99|0.80|
|[[:human_genes:A:AAAS|AAAS]]|2.00|0.66|
|[[:human_genes:T:TNC|TNC]]|2.00|0.66|
|[[:human_genes:P:PABPN1L|PABPN1L]]|2.01|0.66|
|[[:human_genes:U:USP27X|USP27X]]|2.03|0.87|
|[[:human_genes:R:RECK|RECK]]|2.06|0.60|
|[[:human_genes:C:CLCN6|CLCN6]]|2.07|0.60|
|[[:human_genes:C:CDC73|CDC73]]|2.08|0.73|
|[[:human_genes:R:RILPL1|RILPL1]]|2.09|0.73|
|[[:human_genes:P:PSMC3|PSMC3]]|2.14|0.82|
|[[:human_genes:P:PCNA|PCNA]]|2.15|0.87|
|[[:human_genes:T:TRIM52|TRIM52]]|2.15|0.76|
|[[:human_genes:K:KLC4|KLC4]]|2.15|0.60|
|[[:human_genes:A:ANGPT1|ANGPT1]]|2.16|0.60|
|[[:human_genes:O:OR14I1|OR14I1]]|2.17|0.60|
|[[:human_genes:A:ARPC5L|ARPC5L]]|2.18|0.66|
|[[:human_genes:A:ADAM29|ADAM29]]|2.18|0.60|
|[[:human_genes:W:WDR11|WDR11]]|2.24|0.60|
|[[:human_genes:G:GID4|GID4]]|2.28|0.60|
|[[:human_genes:S:SPCS1|SPCS1]]|2.31|0.60|
|[[:human_genes:S:STIL|STIL]]|2.45|0.60|
|[[:human_genes:S:SPC25|SPC25]]|2.46|0.66|
|[[:human_genes:S:SART1|SART1]]|2.65|0.68|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp372|Ibrutinib 1μM R07 exp372]]|0.065|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp486|Heregulin-B 44ng/ml R08 exp486]]|0.058|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp144|PFI-3 10μM R03 exp144]]|0.055|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp185|L-BMAA 500 to 750μM on day4 R04 exp185]]|0.054|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp362|GSK-J4 1μM R07 exp362]]|0.053|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp376|Losmapimod 1μM R07 exp376]]|0.051|<button type="primary" size="small" modal="6">View</button>|
</modal>


<modal id="1" size="lg" title="Ibrutinib 1μM R07 exp372  vs  VHL-ligand-1 20μM R07 exp421">
{{:chemogenomics:comparison_plots:exp372_vs_exp421.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Heregulin-B 44ng/ml R08 exp486  vs  VHL-ligand-1 20μM R07 exp421">
{{:chemogenomics:comparison_plots:exp421_vs_exp486.png?nolink |}}
</modal>

<modal id="3" size="lg" title="PFI-3 10μM R03 exp144  vs  VHL-ligand-1 20μM R07 exp421">
{{:chemogenomics:comparison_plots:exp144_vs_exp421.png?nolink |}}
</modal>

<modal id="4" size="lg" title="L-BMAA 500 to 750μM on day4 R04 exp185  vs  VHL-ligand-1 20μM R07 exp421">
{{:chemogenomics:comparison_plots:exp185_vs_exp421.png?nolink |}}
</modal>

<modal id="5" size="lg" title="GSK-J4 1μM R07 exp362  vs  VHL-ligand-1 20μM R07 exp421">
{{:chemogenomics:comparison_plots:exp362_vs_exp421.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Losmapimod 1μM R07 exp376  vs  VHL-ligand-1 20μM R07 exp421">
{{:chemogenomics:comparison_plots:exp376_vs_exp421.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>