Zebularine 20μM R07 exp423 [The Human Chemical-Genetic Interaction Map Project]

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====== Zebularine 20μM R07 exp423 ======
==== Mechanism of Action ====
Inhibits cytidine deaminase, transition state analog, also inhibits DNMT1 via reversible covalent complex
  * **<color #00a2e8>Class / Subclass 1:</color>** Gene Regulation / Epigenetic Inhibitor




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Zebularine%%
    * **<color #00a2e8>Synonyms:</color>** NSC309132; 4-Deoxyuridine
    * **<color #00a2e8>CAS #:</color>** 3690-10-6
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/100016|100016]]
    * **<color #00a2e8>IUPAC:</color>** %%1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidin-2-one%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C9H12N2O5/c12-4-5-6(13)7(14)8(16-5)11-3-1-2-10-9(11)15/h1-3,5-8,12-14H,4H2/t5-,6-,7-,8-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** RPQZTTQVRYEKCR-WCTZXXKLSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 228.2
    * **<color #00a2e8>Canonical SMILES:</color>** C1=CN(C(=O)N=C1)C2C(C(C(O2)CO)O)O
    * **<color #00a2e8>Isomeric SMILES:</color>** C1=CN(C(=O)N=C1)[C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)O
    * **<color #00a2e8>Molecular Formula:</color>** C9H12N2O5
{{:chemogenomics:structures:chem-0254.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Ark Pharm Inc.
    * **<color #00a2e8>Catalog #:</color>** AK326160
    * **<color #00a2e8>Lot #:</color>** WG0156999-170316001
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+Na)+ Calcd for C9H12N2O5 251.06384; found 251.06358
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Zebularine
    * **<color #00a2e8>Min:</color>** -22.6878; **<color #00a2e8>Max:</color>** 11.3745
{{:chemogenomics:dose_response:dr_478.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |N/A    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 20µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.3
    * **<color #00a2e8>Numbers of reads</color>**: 17023526


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|3/3|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Zebularine_20uM_Round-7_exp423.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="default" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Zebularine 20μM R07 exp423'>
{{:chemogenomics:cranks_plots:exp423.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:N:NUDT15|NUDT15]]|-5.43|<0.01|
|[[:human_genes:T:TDP1|TDP1]]|-3.73|<0.01|
|[[:human_genes:N:NT5C3A|NT5C3A]]|-3.32|<0.01|
|[[:human_genes:T:TMC2|TMC2]]|-2.72|0.07|
|[[:human_genes:O:OSGEP|OSGEP]]|-2.65|0.14|
|[[:human_genes:N:NUDT5|NUDT5]]|-2.54|0.12|
|[[:human_genes:U:UXS1|UXS1]]|-2.50|0.07|
|[[:human_genes:S:SRSF6|SRSF6]]|-2.41|0.18|
|[[:human_genes:R:RSRC2|RSRC2]]|-2.27|0.67|
|[[:human_genes:D:DCTPP1|DCTPP1]]|-2.21|0.22|
|[[:human_genes:Z:ZNF584|ZNF584]]|-2.20|0.22|
|[[:human_genes:H:HTR3C|HTR3C]]|-2.18|0.37|
|[[:human_genes:C:C1orf115|C1orf115]]|-2.14|0.27|
|[[:human_genes:D:DCAF4|DCAF4]]|-2.13|0.27|
|[[:human_genes:Z:ZCCHC10|ZCCHC10]]|-2.08|0.39|
|[[:human_genes:D:DPH5|DPH5]]|-2.05|0.37|
|[[:human_genes:T:TRAPPC3L|TRAPPC3L]]|-2.04|0.37|
|[[:human_genes:I:INTS3|INTS3]]|-2.02|0.49|
|[[:human_genes:R:RHOA|RHOA]]|-2.01|0.39|
|[[:human_genes:O:OR4C15|OR4C15]]|-2.00|0.39|
|[[:human_genes:T:TGS1|TGS1]]|-1.97|0.39|
|[[:human_genes:S:SLC14A2|SLC14A2]]|-1.97|0.56|
|[[:human_genes:D:DPAGT1|DPAGT1]]|-1.96|0.39|
|[[:human_genes:A:ALG5|ALG5]]|-1.96|0.39|
|[[:human_genes:P:PARP1|PARP1]]|-1.94|0.48|
|[[:human_genes:G:GTF3C6|GTF3C6]]|-1.94|0.48|
|[[:human_genes:C:CADPS2|CADPS2]]|-1.94|0.39|
|[[:human_genes:C:C18orf25|C18orf25]]|-1.94|0.39|
|[[:human_genes:X:XRCC2|XRCC2]]|-1.94|0.39|
|[[:human_genes:A:APOH|APOH]]|-1.93|0.39|
|[[:human_genes:S:STX8|STX8]]|1.93|0.61|
|[[:human_genes:P:PSMC2|PSMC2]]|1.96|0.61|
|[[:human_genes:T:TEX33|TEX33]]|2.00|0.47|
|[[:human_genes:P:POLR2C|POLR2C]]|2.01|0.47|
|[[:human_genes:T:TNRC6C|TNRC6C]]|2.03|0.47|
|[[:human_genes:C:C12orf66|C12orf66]]|2.05|0.42|
|[[:human_genes:M:MDH1|MDH1]]|2.06|0.47|
|[[:human_genes:P:PCTP|PCTP]]|2.08|0.47|
|[[:human_genes:A:APLF|APLF]]|2.09|0.36|
|[[:human_genes:M:MYB|MYB]]|2.10|0.61|
|[[:human_genes:S:SETD2|SETD2]]|2.11|0.50|
|[[:human_genes:X:XPO1|XPO1]]|2.12|0.61|
|[[:human_genes:I:ISY1-RAB43|ISY1-RAB43]]|2.14|0.61|
|[[:human_genes:E:EIF5B|EIF5B]]|2.14|0.69|
|[[:human_genes:C:CCNA2|CCNA2]]|2.17|0.47|
|[[:human_genes:R:RPS3|RPS3]]|2.20|0.47|
|[[:human_genes:C:CDC73|CDC73]]|2.23|0.55|
|[[:human_genes:T:TFDP1|TFDP1]]|2.23|0.36|
|[[:human_genes:F:FAM216A|FAM216A]]|2.27|0.19|
|[[:human_genes:S:SNRNP70|SNRNP70]]|2.27|0.33|
|[[:human_genes:R:RAB3GAP1|RAB3GAP1]]|2.28|0.19|
|[[:human_genes:B:BRD1|BRD1]]|2.29|0.19|
|[[:human_genes:D:DDIT4|DDIT4]]|2.33|0.27|
|[[:human_genes:G:GYPE|GYPE]]|2.34|0.47|
|[[:human_genes:K:KPTN|KPTN]]|2.47|0.11|
|[[:human_genes:L:LIG1|LIG1]]|2.53|0.65|
|[[:human_genes:G:GAGE12H|GAGE12H]]|2.77|0.19|
|[[:human_genes:O:OR10G3|OR10G3]]|2.92|0.03|
|[[:human_genes:T:TSC2|TSC2]]|3.10|<0.01|
|[[:human_genes:U:UCK2|UCK2]]|4.72|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
No correlation data above threshold. (correlation > 0.05)
</modal>


<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|DNA-directed RNA polymerase complex|23.27|CDC73,POLR2C,POLR2F,CTR9|
|RNA polymerase complex|22.79|CDC73,POLR2C,POLR2F,CTR9|
|transcription by RNA polymerase II|9.81|TFDP1,CDC73,SETD2,POLR2C,POLR2F,CTR9|
|aromatic compound biosynthetic process|5.44|UCK2,LIG1,TFDP1,CDC73,CCNA2,SETD2,POLR2C,POLR2F,CTR9|
</modal>