Rapamycin 0.001μM R08 exp429 [The Human Chemical-Genetic Interaction Map Project]

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====== Rapamycin 0.001μM R08 exp429 ======
==== Mechanism of Action ====
Allosteric mTORC1 inhibitor, forms ternary complex with FKBP12 and mTOR, attenuates protein synthesis and metabolism
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Kinase Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Proteostasis / mTOR inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Sirolimus%%
    * **<color #00a2e8>Synonyms:</color>** Sirolimus; AY 22989
    * **<color #00a2e8>CAS #:</color>** 53123-88-9
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5284616|5284616]]
    * **<color #00a2e8>IUPAC:</color>** %%(1R,9S,12S,15R,16E,18R,19R,21R,23S,24E,26E,28E,30S,32S,35R)-1,18-dihydroxy-12-[(2R)-1-[(1S,3R,4R)-4-hydroxy-3-methoxycyclohexyl]propan-2-yl]-19,30-dimethoxy-15,17,21,23,29,35-hexamethyl-11,36-dioxa-4-azatricyclo[30.3.1.04,9]hexatriaconta-16,24,26,28-tetraene-2,3,10,14,20-pentone%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C51H79NO13/c1-30-16-12-11-13-17-31(2)42(61-8)28-38-21-19-36(7)51(60,65-38)48(57)49(58)52-23-15-14-18-39(52)50(59)64-43(33(4)26-37-20-22-40(53)44(27-37)62-9)29-41(54)32(3)25-35(6)46(56)47(63-10)45(55)34(5)24-30/h11-13,16-17,25,30,32-34,36-40,42-44,46-47,53,56,60H,14-15,18-24,26-29H2,1-10H3/b13-11+,16-12+,31-17+,35-25+/t30-,32-,33-,34-,36-,37+,38+,39+,40-,42+,43+,44-,46-,47+,51-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** QFJCIRLUMZQUOT-HPLJOQBZSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 914.2
    * **<color #00a2e8>Canonical SMILES:</color>** CC1CCC2CC(C(=CC=CC=CC(CC(C(=O)C(C(C(=CC(C(=O)CC(OC(=O)C3CCCCN3C(=O)C(=O)C1(O2)O)C(C)CC4CCC(C(C4)OC)O)C)C)O)OC)C)C)C)OC
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@@H]1CC[C@H]2C[C@@H](/C(=C/C=C/C=C/[C@H](C[C@H](C(=O)[C@@H]([C@@H](/C(=C/[C@H](C(=O)C[C@H](OC(=O)[C@@H]3CCCCN3C(=O)C(=O)[C@@]1(O2)O)[C@H](C)C[C@@H]4CC[C@H]([C@@H](C4)OC)O)C)/C)O)OC)C)C)/C)OC
    * **<color #00a2e8>Molecular Formula:</color>** C51H79NO13
{{:chemogenomics:structures:chem-0016.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** LC Laboratories
    * **<color #00a2e8>Catalog #:</color>** R-5000
    * **<color #00a2e8>Lot #:</color>** ASW-130
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+Na)+ Calcd for C51H79NO13 936.54436; found 936.54366
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Rapamycin_old
    * **<color #00a2e8>Min:</color>** -12.4006; **<color #00a2e8>Max:</color>** 41.8376
{{:chemogenomics:dose_response:dr_621.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |6.9560    |
| IC20  |8.3100    |
| IC30  |9.3520    |
| IC40  |10.3000    |
| IC50  |11.2600    |
| IC60  |12.3100    |
| IC70  |13.5600    |
| IC80  |15.2600    |
| IC90  |18.2400    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 08
    * **<color #00a2e8>Dose</color>**: 1nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 5.6
    * **<color #00a2e8>Numbers of reads</color>**: 17160031


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|3/13|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Rapamycin_0.001uM_Round-8_exp429.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Rapamycin 0.001μM R08 exp429'>
{{:chemogenomics:cranks_plots:exp429.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:A:ASB3|ASB3]]|-3.44|<0.01|
|[[:human_genes:C:CCDC101|CCDC101]]|-3.18|<0.01|
|[[:human_genes:C:CUL3|CUL3]]|-2.82|0.02|
|[[:human_genes:C:CDIPT|CDIPT]]|-2.76|0.10|
|[[:human_genes:D:DPAGT1|DPAGT1]]|-2.67|0.07|
|[[:human_genes:C:CDK6|CDK6]]|-2.60|0.09|
|[[:human_genes:T:TRIP12|TRIP12]]|-2.57|0.06|
|[[:human_genes:R:RRAGA|RRAGA]]|-2.55|0.09|
|[[:human_genes:N:NOC3L|NOC3L]]|-2.48|0.36|
|[[:human_genes:T:TAF5L|TAF5L]]|-2.44|0.10|
|[[:human_genes:G:GNA13|GNA13]]|-2.42|0.09|
|[[:human_genes:S:SCAF8|SCAF8]]|-2.38|0.09|
|[[:human_genes:L:LAMTOR4|LAMTOR4]]|-2.32|0.10|
|[[:human_genes:C:CREBBP|CREBBP]]|-2.32|0.12|
|[[:human_genes:Z:ZFY|ZFY]]|-2.30|0.10|
|[[:human_genes:A:ACTB|ACTB]]|-2.30|0.10|
|[[:human_genes:G:GPR75-ASB3|GPR75-ASB3]]|-2.29|0.14|
|[[:human_genes:Q:QSER1|QSER1]]|-2.26|0.10|
|[[:human_genes:T:TCF4|TCF4]]|-2.25|0.10|
|[[:human_genes:S:SF3B3|SF3B3]]|-2.24|0.46|
|[[:human_genes:C:CFL1|CFL1]]|-2.21|0.60|
|[[:human_genes:F:FLI1|FLI1]]|-2.19|0.12|
|[[:human_genes:L:LAMTOR2|LAMTOR2]]|-2.19|0.12|
|[[:human_genes:A:AGO2|AGO2]]|-2.18|0.35|
|[[:human_genes:I:ID3|ID3]]|-2.18|0.12|
|[[:human_genes:G:GMEB1|GMEB1]]|-2.15|0.23|
|[[:human_genes:M:MEF2C|MEF2C]]|-2.15|0.23|
|[[:human_genes:L:LUC7L2|LUC7L2]]|-2.14|0.23|
|[[:human_genes:P:POLD3|POLD3]]|-2.13|0.65|
|[[:human_genes:G:GAGE2A|GAGE2A]]|-2.12|0.60|
|[[:human_genes:P:PSMD7|PSMD7]]|2.23|0.34|
|[[:human_genes:A:ARID4A|ARID4A]]|2.24|0.08|
|[[:human_genes:S:SCAP|SCAP]]|2.24|0.08|
|[[:human_genes:K:KAT7|KAT7]]|2.26|0.08|
|[[:human_genes:A:ANKRD11|ANKRD11]]|2.27|0.08|
|[[:human_genes:A:ACMSD|ACMSD]]|2.28|0.08|
|[[:human_genes:P:PAX3|PAX3]]|2.30|0.10|
|[[:human_genes:S:SFXN4|SFXN4]]|2.31|0.07|
|[[:human_genes:C:C9orf156|C9orf156]]|2.32|0.10|
|[[:human_genes:B:BRD1|BRD1]]|2.35|0.06|
|[[:human_genes:P:PEX13|PEX13]]|2.35|0.06|
|[[:human_genes:A:ARID4B|ARID4B]]|2.37|0.08|
|[[:human_genes:M:MCM5|MCM5]]|2.41|0.20|
|[[:human_genes:C:CXorf21|CXorf21]]|2.41|0.08|
|[[:human_genes:C:CXorf56|CXorf56]]|2.49|0.06|
|[[:human_genes:X:XPO1|XPO1]]|2.50|0.34|
|[[:human_genes:R:RFC1|RFC1]]|2.53|0.14|
|[[:human_genes:D:DNTTIP1|DNTTIP1]]|2.62|0.01|
|[[:human_genes:P:PPP3R1|PPP3R1]]|2.68|0.01|
|[[:human_genes:I:ITPR1|ITPR1]]|2.88|<0.01|
|[[:human_genes:F:FAM122A|FAM122A]]|3.15|0.00|
|[[:human_genes:M:MEMO1|MEMO1]]|3.43|0.00|
|[[:human_genes:C:CNOT8|CNOT8]]|3.61|0.00|
|[[:human_genes:G:GID8|GID8]]|3.66|0.00|
|[[:human_genes:Y:YPEL5|YPEL5]]|3.85|0.00|
|[[:human_genes:C:CNOT2|CNOT2]]|3.89|0.00|
|[[:human_genes:W:WDR26|WDR26]]|3.94|0.00|
|[[:human_genes:P:PDS5B|PDS5B]]|3.98|0.00|
|[[:human_genes:U:UBE2H|UBE2H]]|4.01|0.00|
|[[:human_genes:F:FKBP1A|FKBP1A]]|7.81|0.00|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|0.164|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp30|Rapamycin 10μM R00 exp30]]|0.148|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp306|Rapamycin 2μM R07 exp306]]|0.124|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp307|Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307]]|0.078|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp308|Rapamycin 2μM plus FK-506 5μM R07 exp308]]|0.066|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp82|Torin1 0.08μM R02 exp82]]|0.063|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp352|Dexamethasone 0.006 to 0.015μM on day4 R07 exp352]]|0.061|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp229|Dimethyloxaloylglycine 100μM R05 exp229]]|0.06|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp351|Dexamethasone 0.006μM R07 exp351]]|0.06|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp504|MK2206 4μM R08 exp504]]|0.052|<button type="primary" size="small" modal="10">View</button>|
</modal>


<modal id="1" size="lg" title="Rapamycin 0.001μM R08 exp429  vs  Rapamycin 1μM R00 exp29">
{{:chemogenomics:comparison_plots:exp29_vs_exp429.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Rapamycin 0.001μM R08 exp429  vs  Rapamycin 10μM R00 exp30">
{{:chemogenomics:comparison_plots:exp30_vs_exp429.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Rapamycin 0.001μM R08 exp429  vs  Rapamycin 2μM R07 exp306">
{{:chemogenomics:comparison_plots:exp306_vs_exp429.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Rapamycin 0.001μM R08 exp429  vs  Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307">
{{:chemogenomics:comparison_plots:exp307_vs_exp429.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Rapamycin 0.001μM R08 exp429  vs  Rapamycin 2μM plus FK-506 5μM R07 exp308">
{{:chemogenomics:comparison_plots:exp308_vs_exp429.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Rapamycin 0.001μM R08 exp429  vs  Torin1 0.08μM R02 exp82">
{{:chemogenomics:comparison_plots:exp429_vs_exp82.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Dexamethasone 0.006 to 0.015μM on day4 R07 exp352  vs  Rapamycin 0.001μM R08 exp429">
{{:chemogenomics:comparison_plots:exp352_vs_exp429.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Dimethyloxaloylglycine 100μM R05 exp229  vs  Rapamycin 0.001μM R08 exp429">
{{:chemogenomics:comparison_plots:exp229_vs_exp429.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Dexamethasone 0.006μM R07 exp351  vs  Rapamycin 0.001μM R08 exp429">
{{:chemogenomics:comparison_plots:exp351_vs_exp429.png?nolink |}}
</modal>

<modal id="10" size="lg" title="MK2206 4μM R08 exp504  vs  Rapamycin 0.001μM R08 exp429">
{{:chemogenomics:comparison_plots:exp429_vs_exp504.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|GTPase activator complex|184.10|RRAGA,LAMTOR4,LAMTOR2|
|enzyme activator complex|131.50|RRAGA,LAMTOR4,LAMTOR2|
|SAGA complex|76.71|SGF29,TAF5L,SF3B3|
|histone acetylation|63.48|SGF29,TAF5L,CREBBP|
|internal peptidyl-lysine acetylation|54.15|SGF29,TAF5L,CREBBP|
|internal protein amino acid acetylation|49.76|SGF29,TAF5L,CREBBP|
|positive regulation of TORC1 signaling|49.09|CUL3,RRAGA,LAMTOR4,LAMTOR2|
|peptidyl-lysine acetylation|48.45|SGF29,TAF5L,CREBBP|
|SAGA-type complex|48.45|SGF29,TAF5L,SF3B3|
|histone acetyltransferase complex|34.09|SGF29,TAF5L,CREBBP,ACTB,SF3B3|
|cellular response to amino acid stimulus|33.17|CUL3,RRAGA,LAMTOR4,LAMTOR2|
|protein acetyltransferase complex|30.68|SGF29,TAF5L,CREBBP,ACTB,SF3B3|
|acetyltransferase complex|30.38|SGF29,TAF5L,CREBBP,ACTB,SF3B3|
|response to amino acid|21.53|CUL3,RRAGA,LAMTOR4,LAMTOR2|
|intracellular protein-containing complex|7.02|SGF29,CUL3,TAF5L,LAMTOR4,CREBBP,ACTB,SF3B3,LAMTOR2,AGO2,POLD3|
|transferase complex|5.94|SGF29,CUL3,CDK6,TAF5L,CREBBP,ACTB,SF3B3,POLD3|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|intracellular protein-containing complex|8.21|WDR26,CNOT2,YPEL5,GID8,CNOT8,ITPR1,PPP3R1,BRD1,KAT7,RACGAP1,PSMD7,POLE,MAEA|
</modal>