Azithromycin 100μM R08 exp452 [The Human Chemical-Genetic Interaction Map Project]

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====== Azithromycin 100μM R08 exp452 ======
==== Mechanism of Action ====
Binds 50S subunit of bacterial ribosome, inhibits translation
  * **<color #00a2e8>Class / Subclass 1:</color>** Infectious Disease / Antibiotic
  * **<color #00a2e8>Class / Subclass 2:</color>** Proteostasis / Translation Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Azithromycin%%
    * **<color #00a2e8>Synonyms:</color>** CP 62993
    * **<color #00a2e8>CAS #:</color>** 83905-01-5
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/447043|447043]]
    * **<color #00a2e8>IUPAC:</color>** %%(2R,3S,4R,5R,8R,10R,11R,12S,13S,14R)-11-[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy-2-ethyl-3,4,10-trihydroxy-13-[(2R,4R,5S,6S)-5-hydroxy-4-methoxy-4,6-dimethyloxan-2-yl]oxy-3,5,6,8,10,12,14-heptamethyl-1-oxa-6-azacyclopentadecan-15-one%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C38H72N2O12/c1-15-27-38(10,46)31(42)24(6)40(13)19-20(2)17-36(8,45)33(52-35-29(41)26(39(11)12)16-21(3)48-35)22(4)30(23(5)34(44)50-27)51-28-18-37(9,47-14)32(43)25(7)49-28/h20-33,35,41-43,45-46H,15-19H2,1-14H3/t20-,21-,22+,23-,24-,25+,26+,27-,28+,29-,30+,31-,32+,33-,35+,36-,37-,38-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** MQTOSJVFKKJCRP-BICOPXKESA-N
    * **<color #00a2e8>Molecular Weight:</color>** 749
    * **<color #00a2e8>Canonical SMILES:</color>** CCC1C(C(C(N(CC(CC(C(C(C(C(C(=O)O1)C)OC2CC(C(C(O2)C)O)(C)OC)C)OC3C(C(CC(O3)C)N(C)C)O)(C)O)C)C)C)O)(C)O
    * **<color #00a2e8>Isomeric SMILES:</color>** CC[C@@H]1[C@@]([C@@H]([C@H](N(C[C@@H](C[C@@]([C@@H]([C@H]([C@@H]([C@H](C(=O)O1)C)O[C@H]2C[C@@]([C@H]([C@@H](O2)C)O)(C)OC)C)O[C@H]3[C@@H]([C@H](C[C@H](O3)C)N(C)C)O)(C)O)C)C)C)O)(C)O
    * **<color #00a2e8>Molecular Formula:</color>** C38H72N2O12
{{:chemogenomics:structures:chem-0263.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Med Chem Express
    * **<color #00a2e8>Catalog #:</color>** HY-17506
    * **<color #00a2e8>Lot #:</color>** 14100
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C38H72N2O12 749.5158; found 749.51694
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Azithromycin
    * **<color #00a2e8>Min:</color>** -12.7057; **<color #00a2e8>Max:</color>** 42.2848
{{:chemogenomics:dose_response:dr_501.png?nolink&500 |}}
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |32.4700    |
| IC20  |55.4300    |
| IC30  |79.0900    |
| IC40  |105.8000    |
| IC50  |138.3000    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 08
    * **<color #00a2e8>Dose</color>**: 100µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 4.9
    * **<color #00a2e8>Numbers of reads</color>**: 15132253


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|9/6|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Azithromycin_100uM_Round-8_exp452.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Azithromycin 100μM R08 exp452'>
{{:chemogenomics:cranks_plots:exp452.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:S:SLC7A6|SLC7A6]]|-5.33|<0.01|
|[[:human_genes:S:SLC5A3|SLC5A3]]|-3.85|<0.01|
|[[:human_genes:F:FDPS|FDPS]]|-3.78|<0.01|
|[[:human_genes:F:FDFT1|FDFT1]]|-3.58|<0.01|
|[[:human_genes:T:TCF4|TCF4]]|-3.45|<0.01|
|[[:human_genes:B:BRD1|BRD1]]|-3.29|<0.01|
|[[:human_genes:K:KAT7|KAT7]]|-3.02|<0.01|
|[[:human_genes:A:ACAT2|ACAT2]]|-2.89|<0.01|
|[[:human_genes:A:ATP2A2|ATP2A2]]|-2.83|0.32|
|[[:human_genes:F:FLVCR1|FLVCR1]]|-2.78|<0.01|
|[[:human_genes:P:PHF5A|PHF5A]]|-2.74|0.09|
|[[:human_genes:W:WEE1|WEE1]]|-2.67|0.14|
|[[:human_genes:T:TCF3|TCF3]]|-2.66|0.07|
|[[:human_genes:K:KMT2A|KMT2A]]|-2.57|0.06|
|[[:human_genes:W:WSB1|WSB1]]|-2.54|0.06|
|[[:human_genes:S:SOX4|SOX4]]|-2.52|0.09|
|[[:human_genes:S:SLC4A7|SLC4A7]]|-2.48|0.07|
|[[:human_genes:T:TMEM30A|TMEM30A]]|-2.43|0.06|
|[[:human_genes:R:RNGTT|RNGTT]]|-2.41|0.10|
|[[:human_genes:D:DPAGT1|DPAGT1]]|-2.40|0.09|
|[[:human_genes:S:SLC3A2|SLC3A2]]|-2.38|0.09|
|[[:human_genes:C:COL4A3BP|COL4A3BP]]|-2.38|0.06|
|[[:human_genes:A:AEBP2|AEBP2]]|-2.27|0.09|
|[[:human_genes:T:TAAR5|TAAR5]]|-2.25|0.09|
|[[:human_genes:I:ING5|ING5]]|-2.20|0.10|
|[[:human_genes:A:ANKEF1|ANKEF1]]|-2.20|0.13|
|[[:human_genes:G:GFPT1|GFPT1]]|-2.18|0.19|
|[[:human_genes:T:TCF12|TCF12]]|-2.18|0.13|
|[[:human_genes:P:PZP|PZP]]|-2.18|0.10|
|[[:human_genes:P:PXK|PXK]]|-2.17|0.10|
|[[:human_genes:C:C12orf66|C12orf66]]|2.16|0.19|
|[[:human_genes:Y:YPEL5|YPEL5]]|2.16|0.13|
|[[:human_genes:E:EIF3A|EIF3A]]|2.18|0.12|
|[[:human_genes:M:MEMO1|MEMO1]]|2.19|0.12|
|[[:human_genes:C:CFAP45|CFAP45]]|2.20|0.46|
|[[:human_genes:M:ME2|ME2]]|2.20|0.12|
|[[:human_genes:T:TXNL4A|TXNL4A]]|2.23|0.45|
|[[:human_genes:K:KDM1A|KDM1A]]|2.25|0.20|
|[[:human_genes:W:WDR26|WDR26]]|2.25|0.20|
|[[:human_genes:M:MAEA|MAEA]]|2.26|0.09|
|[[:human_genes:S:SEPHS1|SEPHS1]]|2.26|0.09|
|[[:human_genes:S:STRADA|STRADA]]|2.26|0.13|
|[[:human_genes:K:KDM6A|KDM6A]]|2.30|0.08|
|[[:human_genes:R:RHOA|RHOA]]|2.31|0.08|
|[[:human_genes:D:DGCR2|DGCR2]]|2.31|0.08|
|[[:human_genes:H:HSPE1|HSPE1]]|2.31|0.53|
|[[:human_genes:G:GID8|GID8]]|2.32|0.08|
|[[:human_genes:A:ATP2B1|ATP2B1]]|2.33|0.12|
|[[:human_genes:M:MTRF1|MTRF1]]|2.33|0.08|
|[[:human_genes:P:PREPL|PREPL]]|2.38|0.08|
|[[:human_genes:U:UBE2H|UBE2H]]|2.38|0.08|
|[[:human_genes:K:KLF16|KLF16]]|2.46|0.08|
|[[:human_genes:P:PRKAA1|PRKAA1]]|2.47|0.06|
|[[:human_genes:C:CD72|CD72]]|2.68|0.03|
|[[:human_genes:P:PRKAG1|PRKAG1]]|2.74|0.04|
|[[:human_genes:E:EIF3B|EIF3B]]|2.75|0.15|
|[[:human_genes:S:SMS|SMS]]|2.83|0.02|
|[[:human_genes:N:NPRL3|NPRL3]]|3.10|0.02|
|[[:human_genes:D:DEPDC5|DEPDC5]]|3.12|0.01|
|[[:human_genes:T:TNPO1|TNPO1]]|3.30|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp508|NN-Dimethylsphingosine 2.5μM R08 exp508]]|0.093|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp233|EPZ-5676 30μM R05 exp233]]|0.087|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp510|Nicotine 3000μM R08 exp510]]|0.08|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp483|FTY720 3μM R08 exp483]]|0.077|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp155|UNC1999 2μM R03 exp155]]|0.076|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp504|MK2206 4μM R08 exp504]]|0.073|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp470|Chloroquine 32μM R08 exp470]]|0.072|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp433|LJH685 50μM R08 exp433]]|0.068|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp224|CB-839 10μM R05 exp224]]|0.065|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp151|SGC0946 7μM R03 exp151]]|0.06|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp282|Fluvastatin 2.2μM R06 exp282]]|0.054|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|0.054|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp68|Clomiphene 4.4μM R02 exp68]]|0.054|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp344|Chlorpromazine 10μM R07 exp344]]|0.053|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp490|Hydroxychloroquine 30μM R08 exp490]]|0.052|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp234|Ethanol 0.01 R05 exp234]]|0.052|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp530|Thioridazine 5μM R08 exp530]]|0.052|<button type="primary" size="small" modal="17">View</button>|
</modal>


<modal id="1" size="lg" title="Azithromycin 100μM R08 exp452  vs  NN-Dimethylsphingosine 2.5μM R08 exp508">
{{:chemogenomics:comparison_plots:exp452_vs_exp508.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Azithromycin 100μM R08 exp452  vs  EPZ-5676 30μM R05 exp233">
{{:chemogenomics:comparison_plots:exp233_vs_exp452.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Azithromycin 100μM R08 exp452  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp452_vs_exp510.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Azithromycin 100μM R08 exp452  vs  FTY720 3μM R08 exp483">
{{:chemogenomics:comparison_plots:exp452_vs_exp483.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Azithromycin 100μM R08 exp452  vs  UNC1999 2μM R03 exp155">
{{:chemogenomics:comparison_plots:exp155_vs_exp452.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Azithromycin 100μM R08 exp452  vs  MK2206 4μM R08 exp504">
{{:chemogenomics:comparison_plots:exp452_vs_exp504.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Azithromycin 100μM R08 exp452  vs  Chloroquine 32μM R08 exp470">
{{:chemogenomics:comparison_plots:exp452_vs_exp470.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Azithromycin 100μM R08 exp452  vs  LJH685 50μM R08 exp433">
{{:chemogenomics:comparison_plots:exp433_vs_exp452.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Azithromycin 100μM R08 exp452  vs  CB-839 10μM R05 exp224">
{{:chemogenomics:comparison_plots:exp224_vs_exp452.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Azithromycin 100μM R08 exp452  vs  SGC0946 7μM R03 exp151">
{{:chemogenomics:comparison_plots:exp151_vs_exp452.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Azithromycin 100μM R08 exp452  vs  Fluvastatin 2.2μM R06 exp282">
{{:chemogenomics:comparison_plots:exp282_vs_exp452.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Azithromycin 100μM R08 exp452  vs  GSK626616 14μM R08 exp485">
{{:chemogenomics:comparison_plots:exp452_vs_exp485.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Azithromycin 100μM R08 exp452  vs  Clomiphene 4.4μM R02 exp68">
{{:chemogenomics:comparison_plots:exp452_vs_exp68.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Azithromycin 100μM R08 exp452  vs  Chlorpromazine 10μM R07 exp344">
{{:chemogenomics:comparison_plots:exp344_vs_exp452.png?nolink |}}
</modal>

<modal id="15" size="lg" title="Azithromycin 100μM R08 exp452  vs  Hydroxychloroquine 30μM R08 exp490">
{{:chemogenomics:comparison_plots:exp452_vs_exp490.png?nolink |}}
</modal>

<modal id="16" size="lg" title="Azithromycin 100μM R08 exp452  vs  Ethanol 0.01 R05 exp234">
{{:chemogenomics:comparison_plots:exp234_vs_exp452.png?nolink |}}
</modal>

<modal id="17" size="lg" title="Azithromycin 100μM R08 exp452  vs  Thioridazine 5μM R08 exp530">
{{:chemogenomics:comparison_plots:exp452_vs_exp530.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|H3 histone acetyltransferase complex|244.86|BRD1,KAT7,ING5|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>