CFI-400945 25μM R08 exp469 [The Human Chemical-Genetic Interaction Map Project]

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====== CFI-400945 25μM R08 exp469 ======
==== Mechanism of Action ====
Inhibits PLK4, blocks centriole duplication, induces mitotic defects and cell death
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Kinase Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Cell Cycle / Mitotic Inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%CFI-400945 free base%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 1338806-73-7
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/58486178|58486178]]
    * **<color #00a2e8>IUPAC:</color>** %%(2'S,3R)-2'-[3-[(E)-2-[4-[[(2S,6R)-2,6-dimethylmorpholin-4-yl]methyl]phenyl]ethenyl]-1H-indazol-6-yl]-5-methoxyspiro[1H-indole-3,1'-cyclopropane]-2-one%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C33H34N4O3/c1-20-17-37(18-21(2)40-20)19-23-6-4-22(5-7-23)8-12-29-26-11-9-24(14-31(26)36-35-29)28-16-33(28)27-15-25(39-3)10-13-30(27)34-32(33)38/h4-15,20-21,28H,16-19H2,1-3H3,(H,34,38)(H,35,36)/b12-8+/t20-,21+,28-,33-/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** DADASRPKWOGKCU-FVTQAUBDSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 534.6
    * **<color #00a2e8>Canonical SMILES:</color>** CC1CN(CC(O1)C)CC2=CC=C(C=C2)C=CC3=NNC4=C3C=CC(=C4)C5CC56C7=C(C=CC(=C7)OC)NC6=O
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@@H]1CN(C[C@@H](O1)C)CC2=CC=C(C=C2)/C=C/C3=NNC4=C3C=CC(=C4)[C@@H]5C[C@]56C7=C(C=CC(=C7)OC)NC6=O
    * **<color #00a2e8>Molecular Formula:</color>** C33H34N4O3
{{:chemogenomics:structures:chem-0278.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Med Chem Express
    * **<color #00a2e8>Catalog #:</color>** HY-12300
    * **<color #00a2e8>Lot #:</color>** 23771
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C33H34N4O3 535.27037; found 535.27075
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** CFI400945
    * **<color #00a2e8>Min:</color>** -8.6037; **<color #00a2e8>Max:</color>** 76.6696
{{:chemogenomics:dose_response:dr_607.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |19.9700    |
| IC20  |24.7200    |
| IC30  |28.4900    |
| IC40  |32.0000    |
| IC50  |35.6100    |
| IC60  |39.6200    |
| IC70  |44.5100    |
| IC80  |51.3000    |
| IC90  |63.5100    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 08
    * **<color #00a2e8>Dose</color>**: 25µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: -0.4
    * **<color #00a2e8>Numbers of reads</color>**: 14411620


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|29/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/CFI-400945_25uM_Round-8_exp469.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='CFI-400945 25μM R08 exp469'>
{{:chemogenomics:cranks_plots:exp469.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:S:SCYL1|SCYL1]]|-3.97|<0.01|
|[[:human_genes:G:GPR107|GPR107]]|-3.67|<0.01|
|[[:human_genes:G:GOLGA8H|GOLGA8H]]|-3.43|0.04|
|[[:human_genes:S:SLC5A3|SLC5A3]]|-3.39|<0.01|
|[[:human_genes:O:OSBPL9|OSBPL9]]|-3.35|<0.01|
|[[:human_genes:T:TMEM167B|TMEM167B]]|-3.19|<0.01|
|[[:human_genes:O:OSBPL11|OSBPL11]]|-3.07|<0.01|
|[[:human_genes:C:COG7|COG7]]|-3.02|<0.01|
|[[:human_genes:P:PCYT1A|PCYT1A]]|-3.00|<0.01|
|[[:human_genes:Y:YIPF5|YIPF5]]|-2.99|<0.01|
|[[:human_genes:S:SGMS1|SGMS1]]|-2.98|<0.01|
|[[:human_genes:C:CYB561D2|CYB561D2]]|-2.94|<0.01|
|[[:human_genes:A:ATP6V0A2|ATP6V0A2]]|-2.84|<0.01|
|[[:human_genes:C:COPZ1|COPZ1]]|-2.79|<0.01|
|[[:human_genes:U:UNC50|UNC50]]|-2.79|<0.01|
|[[:human_genes:G:GAGE2A|GAGE2A]]|-2.79|0.23|
|[[:human_genes:F:FLVCR1|FLVCR1]]|-2.78|<0.01|
|[[:human_genes:S:SART1|SART1]]|-2.77|0.13|
|[[:human_genes:A:ARL1|ARL1]]|-2.75|<0.01|
|[[:human_genes:Y:YIPF4|YIPF4]]|-2.74|<0.01|
|[[:human_genes:A:ARID4B|ARID4B]]|-2.71|0.01|
|[[:human_genes:V:VKORC1L1|VKORC1L1]]|-2.63|<0.01|
|[[:human_genes:Z:ZBTB7A|ZBTB7A]]|-2.62|0.01|
|[[:human_genes:C:COG5|COG5]]|-2.59|0.02|
|[[:human_genes:T:TM9SF2|TM9SF2]]|-2.54|0.01|
|[[:human_genes:C:COPB2|COPB2]]|-2.50|0.24|
|[[:human_genes:G:GOLGA6L1|GOLGA6L1]]|-2.49|0.46|
|[[:human_genes:D:DDX3X|DDX3X]]|-2.48|0.02|
|[[:human_genes:P:PI4KB|PI4KB]]|-2.47|0.04|
|[[:human_genes:Z:ZNF91|ZNF91]]|-2.47|0.02|
|[[:human_genes:U:URM1|URM1]]|1.98|0.32|
|[[:human_genes:W:WDR77|WDR77]]|1.98|0.38|
|[[:human_genes:P:P2RY4|P2RY4]]|1.99|0.32|
|[[:human_genes:C:C11orf87|C11orf87]]|2.00|0.32|
|[[:human_genes:D:DSCR4|DSCR4]]|2.00|0.32|
|[[:human_genes:R:RRAGA|RRAGA]]|2.00|0.33|
|[[:human_genes:T:TBC1D16|TBC1D16]]|2.06|0.33|
|[[:human_genes:S:SHISA4|SHISA4]]|2.07|0.32|
|[[:human_genes:C:CERCAM|CERCAM]]|2.08|0.26|
|[[:human_genes:O:ODC1|ODC1]]|2.10|0.26|
|[[:human_genes:M:MEAF6|MEAF6]]|2.16|0.33|
|[[:human_genes:C:CDH22|CDH22]]|2.17|0.29|
|[[:human_genes:G:GEMIN8|GEMIN8]]|2.17|0.33|
|[[:human_genes:D:DDX55|DDX55]]|2.20|0.26|
|[[:human_genes:A:ALG13|ALG13]]|2.22|0.26|
|[[:human_genes:P:PHIP|PHIP]]|2.23|0.15|
|[[:human_genes:L:LIN52|LIN52]]|2.31|0.10|
|[[:human_genes:M:MRPS5|MRPS5]]|2.33|0.17|
|[[:human_genes:E:ENO1|ENO1]]|2.34|0.33|
|[[:human_genes:P:PARK2|PARK2]]|2.35|0.09|
|[[:human_genes:T:TAMM41|TAMM41]]|2.36|0.09|
|[[:human_genes:U:UQCR10|UQCR10]]|2.40|0.09|
|[[:human_genes:A:ATP5B|ATP5B]]|2.40|0.09|
|[[:human_genes:P:PAXBP1|PAXBP1]]|2.49|0.09|
|[[:human_genes:R:RPE|RPE]]|2.57|0.09|
|[[:human_genes:S:SARS|SARS]]|2.81|0.39|
|[[:human_genes:S:SUPV3L1|SUPV3L1]]|2.84|0.09|
|[[:human_genes:S:SARS2|SARS2]]|2.97|0.09|
|[[:human_genes:T:TRIAP1|TRIAP1]]|3.07|0.09|
|[[:human_genes:G:GMPPB|GMPPB]]|3.59|0.09|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp68|Clomiphene 4.4μM R02 exp68]]|0.072|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp90|WYE-354 6μM R02 exp90]]|0.07|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp164|Q15 1 to 2μM on day4 R04 exp164]]|0.062|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp344|Chlorpromazine 10μM R07 exp344]]|0.062|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp442|Ibrutinib 10μM R08 exp442]]|0.06|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp79|Q15 2.7μM R02 exp79]]|0.059|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp507|Monensin 0.3μM R08 exp507]]|0.057|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp26|Oligomycin-A 20μM R00 exp26]]|0.052|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp530|Thioridazine 5μM R08 exp530]]|0.051|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|0.051|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp169|BH1 1μM R04 exp169]]|0.08|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp461|BS-181 20μM R08 exp461]]|0.06|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp539|42°C R08 exp539]]|0.056|<button type="primary" size="small" modal="13">View</button>|
</modal>


<modal id="1" size="lg" title="CFI-400945 25μM R08 exp469  vs  Clomiphene 4.4μM R02 exp68">
{{:chemogenomics:comparison_plots:exp469_vs_exp68.png?nolink |}}
</modal>

<modal id="2" size="lg" title="CFI-400945 25μM R08 exp469  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp469_vs_exp90.png?nolink |}}
</modal>

<modal id="3" size="lg" title="CFI-400945 25μM R08 exp469  vs  Q15 1 to 2μM on day4 R04 exp164">
{{:chemogenomics:comparison_plots:exp164_vs_exp469.png?nolink |}}
</modal>

<modal id="4" size="lg" title="CFI-400945 25μM R08 exp469  vs  Chlorpromazine 10μM R07 exp344">
{{:chemogenomics:comparison_plots:exp344_vs_exp469.png?nolink |}}
</modal>

<modal id="5" size="lg" title="CFI-400945 25μM R08 exp469  vs  Ibrutinib 10μM R08 exp442">
{{:chemogenomics:comparison_plots:exp442_vs_exp469.png?nolink |}}
</modal>

<modal id="6" size="lg" title="CFI-400945 25μM R08 exp469  vs  Q15 2.7μM R02 exp79">
{{:chemogenomics:comparison_plots:exp469_vs_exp79.png?nolink |}}
</modal>

<modal id="7" size="lg" title="CFI-400945 25μM R08 exp469  vs  Monensin 0.3μM R08 exp507">
{{:chemogenomics:comparison_plots:exp469_vs_exp507.png?nolink |}}
</modal>

<modal id="8" size="lg" title="CFI-400945 25μM R08 exp469  vs  Oligomycin-A 20μM R00 exp26">
{{:chemogenomics:comparison_plots:exp26_vs_exp469.png?nolink |}}
</modal>

<modal id="9" size="lg" title="CFI-400945 25μM R08 exp469  vs  Thioridazine 5μM R08 exp530">
{{:chemogenomics:comparison_plots:exp469_vs_exp530.png?nolink |}}
</modal>

<modal id="10" size="lg" title="CFI-400945 25μM R08 exp469  vs  Vemurafenib 6.6μM R08 exp434">
{{:chemogenomics:comparison_plots:exp434_vs_exp469.png?nolink |}}
</modal>

<modal id="11" size="lg" title="BH1 1μM R04 exp169  vs  CFI-400945 25μM R08 exp469">
{{:chemogenomics:comparison_plots:exp169_vs_exp469.png?nolink |}}
</modal>

<modal id="12" size="lg" title="BS-181 20μM R08 exp461  vs  CFI-400945 25μM R08 exp469">
{{:chemogenomics:comparison_plots:exp461_vs_exp469.png?nolink |}}
</modal>

<modal id="13" size="lg" title="42°C R08 exp539  vs  CFI-400945 25μM R08 exp469">
{{:chemogenomics:comparison_plots:exp469_vs_exp539.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|COPI-coated vesicle membrane|131.50|SCYL1,COPZ1,COPB2|
|COPI-coated vesicle|87.67|SCYL1,COPZ1,COPB2|
|intra-Golgi vesicle-mediated transport|79.18|COG7,COPZ1,COG5,COPB2|
|retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum|51.14|SCYL1,COG7,COPZ1,COPB2|
|Golgi organization|27.68|GOLGA8H,COG7,YIPF5,ARL1,YIPF4,COG5|
|Golgi vesicle transport|15.62|SCYL1,COG7,YIPF5,COPZ1,YIPF4,COG5,COPB2|
|Golgi apparatus subcompartment|11.80|GOLGA8H,COG7,YIPF5,SGMS1,ARL1,YIPF4,COG5|
|Golgi membrane|9.35|TMEM167B,COG7,SGMS1,ATP6V0A2,COPZ1,UNC50,ARL1,COG5,COPB2,PI4KB|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>