CyclicAMP 200μM R08 exp475 [The Human Chemical-Genetic Interaction Map Project]

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====== CyclicAMP 200μM R08 exp475 ======
==== Mechanism of Action ====
Second messenger, activates PKA, ion channels, cyclic nucleotide-binding proteins
  * **<color #00a2e8>Class / Subclass 1:</color>** Metabolism / Metabolite
  * **<color #00a2e8>Class / Subclass 2:</color>** Signal Transduction / Kinase Activator



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Cyclic AMP%%
    * **<color #00a2e8>Synonyms:</color>** Adenosine 3',5'-cyclophosphate; Cyclic 3',5'-monophosphate adenosine; Cyclic AMP; Adenosine 3',5'-cyclophosphate; Cyclic 3',5'-monophosphate adenosine; Cyclic AMP
    * **<color #00a2e8>CAS #:</color>** 60-92-4
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/6076|6076]]
    * **<color #00a2e8>IUPAC:</color>** %%(4aR,6R,7R,7aS)-6-(6-aminopurin-9-yl)-2-hydroxy-2-oxo-4a,6,7,7a-tetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinin-7-ol%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C10H12N5O6P/c11-8-5-9(13-2-12-8)15(3-14-5)10-6(16)7-4(20-10)1-19-22(17,18)21-7/h2-4,6-7,10,16H,1H2,(H,17,18)(H2,11,12,13)/t4-,6-,7-,10-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** IVOMOUWHDPKRLL-KQYNXXCUSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 329.21
    * **<color #00a2e8>Canonical SMILES:</color>** C1C2C(C(C(O2)N3C=NC4=C(N=CN=C43)N)O)OP(=O)(O1)O
    * **<color #00a2e8>Isomeric SMILES:</color>** C1[C@@H]2[C@H]([C@H]([C@@H](O2)N3C=NC4=C(N=CN=C43)N)O)OP(=O)(O1)O
    * **<color #00a2e8>Molecular Formula:</color>** C10H12N5O6P
{{:chemogenomics:structures:chem-0284.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Combi-Blocks
    * **<color #00a2e8>Catalog #:</color>** QB-1283
    * **<color #00a2e8>Lot #:</color>** B78133
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C10H12N5O6P 330.0598; found 330.05915
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** cAMP
    * **<color #00a2e8>Min:</color>** 6.4062; **<color #00a2e8>Max:</color>** 96.7733
{{:chemogenomics:dose_response:dr_605.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |104.8000    |
| IC20  |145.7000    |
| IC30  |181.3000    |
| IC40  |217.0000    |
| IC50  |255.8000    |
| IC60  |301.7000    |
| IC70  |361.0000    |
| IC80  |449.4000    |
| IC90  |624.8000    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 08
    * **<color #00a2e8>Dose</color>**: 200µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.2
    * **<color #00a2e8>Numbers of reads</color>**: 14430295


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|2/5|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Cyclic_AMP_200uM_Round-8_exp475.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="default" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='CyclicAMP 200μM R08 exp475'>
{{:chemogenomics:cranks_plots:exp475.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:A:AMPD2|AMPD2]]|-4.40|<0.01|
|[[:human_genes:X:XPO1|XPO1]]|-2.97|0.37|
|[[:human_genes:U:UXS1|UXS1]]|-2.93|0.01|
|[[:human_genes:U:UCK2|UCK2]]|-2.64|0.05|
|[[:human_genes:G:GEMIN8|GEMIN8]]|-2.50|0.24|
|[[:human_genes:E:EMC2|EMC2]]|-2.46|0.11|
|[[:human_genes:A:ATP6V1H|ATP6V1H]]|-2.39|0.41|
|[[:human_genes:B:BHMT2|BHMT2]]|-2.38|0.15|
|[[:human_genes:N:NT5C2|NT5C2]]|-2.36|0.15|
|[[:human_genes:D:DPYD|DPYD]]|-2.26|0.28|
|[[:human_genes:G:GET4|GET4]]|-2.25|0.24|
|[[:human_genes:O:OTUB1|OTUB1]]|-2.22|0.31|
|[[:human_genes:Z:ZBTB21|ZBTB21]]|-2.20|0.24|
|[[:human_genes:A:ABTB1|ABTB1]]|-2.17|0.26|
|[[:human_genes:G:GPR75-ASB3|GPR75-ASB3]]|-2.17|0.37|
|[[:human_genes:A:ARHGAP5|ARHGAP5]]|-2.14|0.28|
|[[:human_genes:K:KIAA2026|KIAA2026]]|-2.05|0.45|
|[[:human_genes:G:GMNN|GMNN]]|-2.04|0.37|
|[[:human_genes:C:CDK1|CDK1]]|-2.03|0.53|
|[[:human_genes:P:PDE4B|PDE4B]]|-2.01|0.39|
|[[:human_genes:G:GRB14|GRB14]]|-2.00|0.39|
|[[:human_genes:N:NAA38|NAA38]]|-1.98|0.62|
|[[:human_genes:T:TMEM107|TMEM107]]|-1.98|0.41|
|[[:human_genes:T:TMEM119|TMEM119]]|-1.96|0.51|
|[[:human_genes:A:ADH7|ADH7]]|-1.96|0.41|
|[[:human_genes:D:DNAJA2|DNAJA2]]|-1.95|0.67|
|[[:human_genes:G:GCNT2|GCNT2]]|-1.91|0.49|
|[[:human_genes:L:LCMT2|LCMT2]]|-1.90|0.53|
|[[:human_genes:M:METAP2|METAP2]]|-1.90|0.67|
|[[:human_genes:A:ALKBH3|ALKBH3]]|-1.90|0.50|
|[[:human_genes:N:NOP16|NOP16]]|1.96|0.76|
|[[:human_genes:T:TSC1|TSC1]]|1.96|0.53|
|[[:human_genes:P:PI4KA|PI4KA]]|1.96|0.53|
|[[:human_genes:M:MIER2|MIER2]]|1.97|0.53|
|[[:human_genes:R:RNF214|RNF214]]|1.97|0.53|
|[[:human_genes:P:POP1|POP1]]|2.00|0.61|
|[[:human_genes:P:PFAS|PFAS]]|2.03|0.80|
|[[:human_genes:P:PSMD6|PSMD6]]|2.05|0.72|
|[[:human_genes:K:KLHL31|KLHL31]]|2.08|0.42|
|[[:human_genes:P:PNO1|PNO1]]|2.10|0.68|
|[[:human_genes:R:ROCK2|ROCK2]]|2.11|0.51|
|[[:human_genes:Z:ZNHIT2|ZNHIT2]]|2.11|0.53|
|[[:human_genes:P:PRDM1|PRDM1]]|2.13|0.49|
|[[:human_genes:G:GART|GART]]|2.13|0.78|
|[[:human_genes:D:DNA2|DNA2]]|2.16|0.61|
|[[:human_genes:R:RPAP2|RPAP2]]|2.19|0.42|
|[[:human_genes:S:SHMT2|SHMT2]]|2.20|0.53|
|[[:human_genes:B:BBS9|BBS9]]|2.20|0.42|
|[[:human_genes:H:HARBI1|HARBI1]]|2.20|0.37|
|[[:human_genes:A:AXIN1|AXIN1]]|2.21|0.42|
|[[:human_genes:M:MDH2|MDH2]]|2.22|0.42|
|[[:human_genes:S:SUGT1|SUGT1]]|2.23|0.53|
|[[:human_genes:C:CMYA5|CMYA5]]|2.31|0.45|
|[[:human_genes:C:CATSPER3|CATSPER3]]|2.47|0.37|
|[[:human_genes:R:RRM2|RRM2]]|2.57|0.72|
|[[:human_genes:N:NUDT2|NUDT2]]|2.74|0.02|
|[[:human_genes:G:G6PD|G6PD]]|2.92|<0.01|
|[[:human_genes:A:ATIC|ATIC]]|3.58|<0.01|
|[[:human_genes:S:SLC29A1|SLC29A1]]|3.86|<0.01|
|[[:human_genes:A:ADK|ADK]]|5.07|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
No correlation data above threshold. (correlation > 0.05)
</modal>


<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|ribonucleoside monophosphate metabolic process|42.32|AMPD2,UCK2,NT5C2,DPYD|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|de novo IMP biosynthetic process|331.38|ATIC,GART,PFAS|
|de novo XMP biosynthetic process|276.15|ATIC,GART,PFAS|
|XMP biosynthetic process|276.15|ATIC,GART,PFAS|
|de novo AMP biosynthetic process|236.70|ATIC,GART,PFAS|
|hydroxymethyl-, formyl- and related transferase activity|236.70|ATIC,SHMT2,GART|
|AMP biosynthetic process|230.12|ADK,ATIC,NUDT2,GART,PFAS|
|GMP biosynthetic process|169.94|ADK,ATIC,GART,PFAS|
|IMP biosynthetic process|165.69|ATIC,GART,PFAS|
|purine ribonucleoside monophosphate biosynthetic process|153.42|ADK,ATIC,NUDT2,GART,PFAS|
|purine nucleoside monophosphate biosynthetic process|110.46|ADK,ATIC,NUDT2,PFAS|
|AMP metabolic process|100.42|ADK,NUDT2,GART,PFAS|
|ribonucleoside monophosphate biosynthetic process|83.68|ADK,ATIC,NUDT2,GART,PFAS|
|nucleoside monophosphate biosynthetic process|69.04|ADK,ATIC,NUDT2,GART,PFAS|
|purine ribonucleoside monophosphate metabolic process|69.04|ADK,ATIC,NUDT2,GART,PFAS|
|purine nucleoside monophosphate metabolic process|65.75|ADK,ATIC,NUDT2,GART,PFAS|
|ribonucleoside monophosphate metabolic process|47.61|ADK,ATIC,NUDT2,GART,PFAS|
|nucleoside monophosphate metabolic process|36.82|ADK,ATIC,NUDT2,GART,PFAS|
|purine-containing compound metabolic process|10.40|ADK,ATIC,G6PD,NUDT2,MDH2,SHMT2,GART,PFAS|
|nucleobase-containing small molecule metabolic process|9.33|ADK,ATIC,G6PD,NUDT2,RRM2,MDH2,SHMT2,GART,PFAS|
</modal>