LY2090314 0.003μM R08 exp499 [The Human Chemical-Genetic Interaction Map Project]

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====== LY2090314 0.003μM R08 exp499 ======
==== Mechanism of Action ====
Inhibits glycogen synthase kinase-3 (GSK-3), activates Wnt signalling
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Kinase Inhibitor




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%3-[6-Fluoro-10-(piperidine-1-carbonyl)-1,10-diazatricyclo[6.4.1.04,13]trideca-2,4,6,8(13)-tetraen-3-yl]-4-imidazo[1,2-a]pyridin-3-ylpyrrole-2,5-dione%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 603288-22-8
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/10029385|10029385]]
    * **<color #00a2e8>IUPAC:</color>** %%3-[6-fluoro-10-(piperidine-1-carbonyl)-1,10-diazatricyclo[6.4.1.04,13]trideca-2,4,6,8(13)-tetraen-3-yl]-4-imidazo[1,2-a]pyridin-3-ylpyrrole-2,5-dione%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C28H25FN6O3/c29-18-12-17-15-34(28(38)32-7-3-1-4-8-32)11-10-33-16-20(19(13-18)25(17)33)23-24(27(37)31-26(23)36)21-14-30-22-6-2-5-9-35(21)22/h2,5-6,9,12-14,16H,1,3-4,7-8,10-11,15H2,(H,31,36,37)
    * **<color #00a2e8>INCHI Key:</color>** HRJWTAWVFDCTGO-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 512.5
    * **<color #00a2e8>Canonical SMILES:</color>** C1CCN(CC1)C(=O)N2CCN3C=C(C4=CC(=CC(=C43)C2)F)C5=C(C(=O)NC5=O)C6=CN=C7N6C=CC=C7
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C28H25FN6O3
{{:chemogenomics:structures:chem-0305.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Med Chem Express
    * **<color #00a2e8>Catalog #:</color>** HY-16294
    * **<color #00a2e8>Lot #:</color>** 11009
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C28H25FN6O3 513.20449; found 513.208
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** LY2090314
    * **<color #00a2e8>Min:</color>** -16.6945; **<color #00a2e8>Max:</color>** 87.6124
{{:chemogenomics:dose_response:dr_614.png?nolink&500 |}}
\\ 
\\ 
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |0.0026    |
| IC20  |0.0033    |
| IC30  |0.0039    |
| IC40  |0.0044    |
| IC50  |0.0050    |
| IC60  |0.0056    |
| IC70  |0.0064    |
| IC80  |0.0076    |
| IC90  |0.0096    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 08
    * **<color #00a2e8>Dose</color>**: 3nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 2.1
    * **<color #00a2e8>Numbers of reads</color>**: 16687176


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|56/94|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/LY2090314_0.003uM_Round-8_exp499.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='LY2090314 0.003μM R08 exp499'>
{{:chemogenomics:cranks_plots:exp499.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:G:GSK3B|GSK3B]]|-5.48|0.00|
|[[:human_genes:N:NPRL3|NPRL3]]|-4.35|0.00|
|[[:human_genes:M:MTF2|MTF2]]|-3.98|0.00|
|[[:human_genes:D:DEPDC5|DEPDC5]]|-3.90|0.00|
|[[:human_genes:I:ITFG2|ITFG2]]|-3.75|0.00|
|[[:human_genes:R:RING1|RING1]]|-3.68|0.00|
|[[:human_genes:M:MSI2|MSI2]]|-3.62|0.00|
|[[:human_genes:S:SZT2|SZT2]]|-3.60|0.00|
|[[:human_genes:N:NPRL2|NPRL2]]|-3.53|0.00|
|[[:human_genes:O:OTUB1|OTUB1]]|-3.37|0.00|
|[[:human_genes:I:ILF3|ILF3]]|-3.22|0.02|
|[[:human_genes:T:TCF4|TCF4]]|-3.21|0.00|
|[[:human_genes:C:C12orf66|C12orf66]]|-3.14|<0.01|
|[[:human_genes:I:IVNS1ABP|IVNS1ABP]]|-3.12|0.00|
|[[:human_genes:P:PRPS1|PRPS1]]|-3.08|0.00|
|[[:human_genes:Z:ZBTB24|ZBTB24]]|-3.03|<0.01|
|[[:human_genes:F:FAM175B|FAM175B]]|-3.02|<0.01|
|[[:human_genes:P:PRKAA1|PRKAA1]]|-3.00|<0.01|
|[[:human_genes:T:TCF3|TCF3]]|-2.93|<0.01|
|[[:human_genes:C:CARM1|CARM1]]|-2.91|<0.01|
|[[:human_genes:D:DPH2|DPH2]]|-2.84|<0.01|
|[[:human_genes:B:BCL6|BCL6]]|-2.80|<0.01|
|[[:human_genes:C:CCDC130|CCDC130]]|-2.78|<0.01|
|[[:human_genes:E:ELAVL1|ELAVL1]]|-2.77|<0.01|
|[[:human_genes:B:BACH2|BACH2]]|-2.75|<0.01|
|[[:human_genes:A:ADK|ADK]]|-2.61|<0.01|
|[[:human_genes:C:CSTB|CSTB]]|-2.61|<0.01|
|[[:human_genes:S:STK11|STK11]]|-2.58|0.02|
|[[:human_genes:R:RSRC2|RSRC2]]|-2.57|0.21|
|[[:human_genes:P:PRMT7|PRMT7]]|-2.56|0.01|
|[[:human_genes:P:PPP3CA|PPP3CA]]|3.16|0.00|
|[[:human_genes:N:NDC80|NDC80]]|3.27|<0.01|
|[[:human_genes:I:ING1|ING1]]|3.30|0.00|
|[[:human_genes:H:HDAC1|HDAC1]]|3.36|0.00|
|[[:human_genes:B:BRD1|BRD1]]|3.41|0.00|
|[[:human_genes:P:PPP3R1|PPP3R1]]|3.41|0.00|
|[[:human_genes:M:MTOR|MTOR]]|3.42|0.00|
|[[:human_genes:R:RPTOR|RPTOR]]|3.45|0.00|
|[[:human_genes:S:SRM|SRM]]|3.47|0.00|
|[[:human_genes:C:CDKN1B|CDKN1B]]|3.51|0.00|
|[[:human_genes:G:GCN1L1|GCN1L1]]|3.52|0.00|
|[[:human_genes:T:TSC22D2|TSC22D2]]|3.55|0.00|
|[[:human_genes:A:ATP2B1|ATP2B1]]|3.59|0.00|
|[[:human_genes:P:PDS5A|PDS5A]]|3.61|0.00|
|[[:human_genes:B:BCL9|BCL9]]|3.62|0.00|
|[[:human_genes:M:MLST8|MLST8]]|3.62|0.00|
|[[:human_genes:D:DYRK1A|DYRK1A]]|3.68|0.00|
|[[:human_genes:N:NFRKB|NFRKB]]|4.08|0.00|
|[[:human_genes:R:RB1|RB1]]|4.16|0.00|
|[[:human_genes:T:TNKS2|TNKS2]]|4.45|0.00|
|[[:human_genes:D:DOHH|DOHH]]|4.69|0.00|
|[[:human_genes:F:FOXK1|FOXK1]]|4.84|0.00|
|[[:human_genes:A:ARID1A|ARID1A]]|4.90|0.00|
|[[:human_genes:K:KLF16|KLF16]]|4.99|0.00|
|[[:human_genes:N:NSD1|NSD1]]|5.67|0.00|
|[[:human_genes:M:MBNL1|MBNL1]]|5.92|0.00|
|[[:human_genes:I:ID3|ID3]]|6.11|0.00|
|[[:human_genes:J:JUP|JUP]]|7.75|0.00|
|[[:human_genes:C:CTNNB1|CTNNB1]]|7.92|0.00|
|[[:human_genes:L:LEF1|LEF1]]|8.26|0.00|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|0.448|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp21|MLN-4924 0.2μM R00 exp21]]|0.102|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|0.065|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp502|Milciclib 2μM R08 exp502]]|0.065|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|0.06|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp242|Radicicol 0.16μM R05 exp242]]|0.054|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp106|UM131593 0.2μM R03 exp106]]|0.054|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp80|RO-3307 4.7μM R02 exp80]]|0.051|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp526|Sulforaphane 9μM R08 exp526 no dilution day6]]|0.051|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp435|JQ1 0.8μM R08 exp435]]|0.059|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|0.059|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp447|Amiloride 100μM R08 exp447]]|0.059|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|0.058|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|0.058|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp234|Ethanol 0.01 R05 exp234]]|0.056|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|0.055|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|0.055|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|0.055|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp365|I-BRD9 4μM R07 exp365]]|0.054|<button type="primary" size="small" modal="19">View</button>|
|[[:results:exp211|AICAR 240μM R05 exp211]]|0.052|<button type="primary" size="small" modal="20">View</button>|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|0.051|<button type="primary" size="small" modal="21">View</button>|
</modal>


<modal id="1" size="lg" title="LY2090314 0.003μM R08 exp499  vs  LY2090314 0.003μM R08 exp500 no dilution day6">
{{:chemogenomics:comparison_plots:exp499_vs_exp500.png?nolink |}}
</modal>

<modal id="2" size="lg" title="LY2090314 0.003μM R08 exp499  vs  MLN-4924 0.2μM R00 exp21">
{{:chemogenomics:comparison_plots:exp21_vs_exp499.png?nolink |}}
</modal>

<modal id="3" size="lg" title="LY2090314 0.003μM R08 exp499  vs  Quercetin 20μM R08 exp517">
{{:chemogenomics:comparison_plots:exp499_vs_exp517.png?nolink |}}
</modal>

<modal id="4" size="lg" title="LY2090314 0.003μM R08 exp499  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp499_vs_exp502.png?nolink |}}
</modal>

<modal id="5" size="lg" title="LY2090314 0.003μM R08 exp499  vs  TRAIL 5ng/ml R00 exp35">
{{:chemogenomics:comparison_plots:exp35_vs_exp499.png?nolink |}}
</modal>

<modal id="6" size="lg" title="LY2090314 0.003μM R08 exp499  vs  Radicicol 0.16μM R05 exp242">
{{:chemogenomics:comparison_plots:exp242_vs_exp499.png?nolink |}}
</modal>

<modal id="7" size="lg" title="LY2090314 0.003μM R08 exp499  vs  UM131593 0.2μM R03 exp106">
{{:chemogenomics:comparison_plots:exp106_vs_exp499.png?nolink |}}
</modal>

<modal id="8" size="lg" title="LY2090314 0.003μM R08 exp499  vs  RO-3307 4.7μM R02 exp80">
{{:chemogenomics:comparison_plots:exp499_vs_exp80.png?nolink |}}
</modal>

<modal id="9" size="lg" title="LY2090314 0.003μM R08 exp499  vs  Sulforaphane 9μM R08 exp526 no dilution day6">
{{:chemogenomics:comparison_plots:exp499_vs_exp526.png?nolink |}}
</modal>

<modal id="10" size="lg" title="JQ1 0.8μM R08 exp435  vs  LY2090314 0.003μM R08 exp499">
{{:chemogenomics:comparison_plots:exp435_vs_exp499.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Hippuristanol 0.12μM R08 exp489 no dilution day6  vs  LY2090314 0.003μM R08 exp499">
{{:chemogenomics:comparison_plots:exp489_vs_exp499.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Amiloride 100μM R08 exp447  vs  LY2090314 0.003μM R08 exp499">
{{:chemogenomics:comparison_plots:exp447_vs_exp499.png?nolink |}}
</modal>

<modal id="13" size="lg" title="3-Bromopyruvate 7μM R04 exp175  vs  LY2090314 0.003μM R08 exp499">
{{:chemogenomics:comparison_plots:exp175_vs_exp499.png?nolink |}}
</modal>

<modal id="14" size="lg" title="BVD-523 15μM R02 exp67  vs  LY2090314 0.003μM R08 exp499">
{{:chemogenomics:comparison_plots:exp499_vs_exp67.png?nolink |}}
</modal>

<modal id="15" size="lg" title="Ethanol 0.01 R05 exp234  vs  LY2090314 0.003μM R08 exp499">
{{:chemogenomics:comparison_plots:exp234_vs_exp499.png?nolink |}}
</modal>

<modal id="16" size="lg" title="CB-5083 0.4μM R08 exp468  vs  LY2090314 0.003μM R08 exp499">
{{:chemogenomics:comparison_plots:exp468_vs_exp499.png?nolink |}}
</modal>

<modal id="17" size="lg" title="Aphidicolin 0.4μM R08 exp440  vs  LY2090314 0.003μM R08 exp499">
{{:chemogenomics:comparison_plots:exp440_vs_exp499.png?nolink |}}
</modal>

<modal id="18" size="lg" title="Actinomycin-D 0.001μM R00 exp3  vs  LY2090314 0.003μM R08 exp499">
{{:chemogenomics:comparison_plots:exp3_vs_exp499.png?nolink |}}
</modal>

<modal id="19" size="lg" title="I-BRD9 4μM R07 exp365  vs  LY2090314 0.003μM R08 exp499">
{{:chemogenomics:comparison_plots:exp365_vs_exp499.png?nolink |}}
</modal>

<modal id="20" size="lg" title="AICAR 240μM R05 exp211  vs  LY2090314 0.003μM R08 exp499">
{{:chemogenomics:comparison_plots:exp211_vs_exp499.png?nolink |}}
</modal>

<modal id="21" size="lg" title="Artemisinin 50μM R08 exp450  vs  LY2090314 0.003μM R08 exp499">
{{:chemogenomics:comparison_plots:exp450_vs_exp499.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|GATOR1 complex|637.27|NPRL3,NPRL2|
|Seh1-associated complex|273.12|NPRL3,DEPDC5,NPRL2|
|negative regulation of TORC1 signaling|108.80|NPRL3,DEPDC5,ITFG2,SZT2,NPRL2,PRKAA1,STK11|
|negative regulation of TOR signaling|67.59|NPRL3,DEPDC5,ITFG2,SZT2,NPRL2,PRKAA1,STK11|
|cellular response to amino acid starvation|66.38|NPRL3,DEPDC5,ITFG2,SZT2,NPRL2|
|response to amino acid starvation|62.48|NPRL3,DEPDC5,ITFG2,SZT2,NPRL2|
|regulation of TORC1 signaling|59.28|NPRL3,DEPDC5,ITFG2,SZT2,NPRL2,OTUB1,PRKAA1,STK11|
|cellular response to glucose starvation|39.83|ITFG2,SZT2,PRKAA1|
|regulation of TOR signaling|33.04|NPRL3,DEPDC5,ITFG2,SZT2,NPRL2,PRKAA1,STK11|
|positive regulation of autophagy|26.19|GSK3B,NPRL3,DEPDC5,NPRL2,PRKAA1,STK11|
|cellular response to starvation|22.36|NPRL3,DEPDC5,ITFG2,SZT2,NPRL2,PRKAA1|
|response to starvation|18.21|NPRL3,DEPDC5,ITFG2,SZT2,NPRL2,PRKAA1|
|cellular response to nutrient levels|16.41|NPRL3,DEPDC5,ITFG2,SZT2,NPRL2,PRKAA1|
|cellular response to extracellular stimulus|14.59|NPRL3,DEPDC5,ITFG2,SZT2,NPRL2,PRKAA1|
|negative regulation of intracellular signal transduction|12.45|GSK3B,NPRL3,DEPDC5,ITFG2,SZT2,NPRL2,IVNS1ABP,PRKAA1,BCL6,STK11|
|positive regulation of cellular catabolic process|12.22|GSK3B,NPRL3,DEPDC5,NPRL2,PRKAA1,STK11|
|cellular response to external stimulus|11.69|NPRL3,DEPDC5,ITFG2,SZT2,NPRL2,PRKAA1|
|regulation of autophagy|10.99|GSK3B,NPRL3,DEPDC5,NPRL2,PRKAA1,STK11|
|regulation of cellular catabolic process|6.93|GSK3B,NPRL3,DEPDC5,NPRL2,PRKAA1,ELAVL1,STK11|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|positive regulation of pentose-phosphate shunt|552.30|MLST8,RPTOR|
|regulation of pentose-phosphate shunt|276.15|MLST8,RPTOR,MTOR|
|regulation of NADP metabolic process|236.70|MLST8,RPTOR,MTOR|
|TOR complex|165.69|MLST8,RPTOR,MTOR|
|TORC1 signaling|150.63|MLST8,RPTOR,MTOR|
|beta-catenin-TCF complex|138.08|LEF1,CTNNB1,BCL9|
|calcineurin-NFAT signaling cascade|118.35|MTOR,PPP3R1,PPP3CA|
|positive regulation of purine nucleotide metabolic process|97.46|MLST8,RPTOR,MTOR|
|positive regulation of myeloid leukocyte differentiation|38.76|LEF1,RB1,RPTOR,PPP3CA|
|positive regulation of myeloid cell differentiation|28.77|LEF1,RB1,RPTOR,BRD1,PPP3CA|
|regulation of myeloid leukocyte differentiation|28.08|LEF1,CTNNB1,RB1,RPTOR,MTOR,PPP3CA|
|regulation of myeloid cell differentiation|20.56|LEF1,CTNNB1,RB1,RPTOR,MTOR,BRD1,PPP3CA|
|regulation of leukocyte differentiation|12.35|LEF1,CTNNB1,ARID1A,RB1,RPTOR,MTOR,PPP3CA|
|regulation of hemopoiesis|11.36|LEF1,CTNNB1,ARID1A,RB1,RPTOR,MTOR,BRD1,PPP3CA|
|positive regulation of cell development|10.16|LEF1,CTNNB1,ARID1A,RB1,RPTOR,MTOR,HDAC1,PPP3CA|
|DNA-binding transcription factor binding|9.42|LEF1,CTNNB1,JUP,ID3,NSD1,ARID1A,RB1,HDAC1|
|transcription factor binding|9.39|LEF1,CTNNB1,JUP,ID3,NSD1,ARID1A,RB1,RPTOR,MTOR,HDAC1|
|transcription coregulator activity|9.07|CTNNB1,JUP,NSD1,ARID1A,RB1,DYRK1A,BCL9,HDAC1|
|muscle structure development|8.87|CTNNB1,ID3,MBNL1,FOXK1,RB1,BCL9,MTOR,PPP3CA|
</modal>