Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53 [The Human Chemical-Genetic Interaction Map Project]

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====== Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53 ======
==== Mechanism of Action ====
Inhibits class I, II, IV HDACs, chelates active site zinc ion
  * **<color #00a2e8>Class / Subclass 1:</color>** Gene Regulation / Epigenetic Inhibitor




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Vorinostat%%
    * **<color #00a2e8>Synonyms:</color>** SAHA
    * **<color #00a2e8>CAS #:</color>** 149647-78-9
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5311|5311]]
    * **<color #00a2e8>IUPAC:</color>** %%N'-hydroxy-N-phenyloctanediamide%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C14H20N2O3/c17-13(15-12-8-4-3-5-9-12)10-6-1-2-7-11-14(18)16-19/h3-5,8-9,19H,1-2,6-7,10-11H2,(H,15,17)(H,16,18)
    * **<color #00a2e8>INCHI Key:</color>** WAEXFXRVDQXREF-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 264.32
    * **<color #00a2e8>Canonical SMILES:</color>** C1=CC=C(C=C1)NC(=O)CCCCCCC(=O)NO
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C14H20N2O3
{{:chemogenomics:structures:chem-0033.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Tocris Bioscience
    * **<color #00a2e8>Catalog #:</color>** 4652
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C14H20N2O3 265.15467; found 265.15439
</panel>


<panel title='Dose Response Curve'>
Dose response curve not available.
</panel>


</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 01
    * **<color #00a2e8>Dose</color>**: 20nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 7.1
    * **<color #00a2e8>Numbers of reads</color>**: 9593321


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|0/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Suberoylanilide-Hydroxamic-Acid_0.02uM_Round-1_exp53.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53'>
{{:chemogenomics:cranks_plots:exp53.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:C:CXorf67|CXorf67]]|-2.92|0.24|
|[[:human_genes:S:STT3A|STT3A]]|-2.84|0.58|
|[[:human_genes:D:DLG3|DLG3]]|-2.68|0.57|
|[[:human_genes:G:GMPPB|GMPPB]]|-2.64|0.54|
|[[:human_genes:U:UBA3|UBA3]]|-2.52|0.74|
|[[:human_genes:C:CBX3|CBX3]]|-2.33|0.54|
|[[:human_genes:V:VEZT|VEZT]]|-2.33|0.54|
|[[:human_genes:C:C10orf90|C10orf90]]|-2.29|0.74|
|[[:human_genes:O:OIP5|OIP5]]|-2.28|0.57|
|[[:human_genes:C:CAD|CAD]]|-2.22|0.70|
|[[:human_genes:G:GGCX|GGCX]]|-2.22|0.54|
|[[:human_genes:N:NBAS|NBAS]]|-2.22|0.54|
|[[:human_genes:P:PCYT1B|PCYT1B]]|-2.20|0.58|
|[[:human_genes:Z:ZNF391|ZNF391]]|-2.19|0.54|
|[[:human_genes:R:RUNX1|RUNX1]]|-2.17|0.74|
|[[:human_genes:K:KPNA5|KPNA5]]|-2.17|0.75|
|[[:human_genes:T:TRAPPC4|TRAPPC4]]|-2.17|0.63|
|[[:human_genes:R:RIC3|RIC3]]|-2.15|0.64|
|[[:human_genes:M:MORN1|MORN1]]|-2.12|0.64|
|[[:human_genes:A:AHCY|AHCY]]|-2.12|0.57|
|[[:human_genes:T:TXNL4B|TXNL4B]]|-2.10|0.64|
|[[:human_genes:T:TGS1|TGS1]]|-2.08|0.54|
|[[:human_genes:C:CUL2|CUL2]]|-2.08|0.74|
|[[:human_genes:H:HCFC1R1|HCFC1R1]]|-2.06|0.74|
|[[:human_genes:C:CLP1|CLP1]]|-2.06|0.74|
|[[:human_genes:B:BCORL1|BCORL1]]|-2.06|0.74|
|[[:human_genes:O:ORAOV1|ORAOV1]]|-2.06|0.58|
|[[:human_genes:T:TOP1|TOP1]]|-2.06|0.74|
|[[:human_genes:D:DDX39A|DDX39A]]|-2.05|0.57|
|[[:human_genes:A:AKR1C1|AKR1C1]]|-2.03|0.74|
|[[:human_genes:P:PSMD7|PSMD7]]|2.14|0.74|
|[[:human_genes:P:PSMB1|PSMB1]]|2.15|0.55|
|[[:human_genes:C:COPS4|COPS4]]|2.16|0.63|
|[[:human_genes:Z:ZNF598|ZNF598]]|2.17|0.55|
|[[:human_genes:C:CNOT3|CNOT3]]|2.19|0.55|
|[[:human_genes:P:PSMD6|PSMD6]]|2.26|0.58|
|[[:human_genes:Z:ZNF397|ZNF397]]|2.27|0.37|
|[[:human_genes:N:NUP155|NUP155]]|2.28|0.57|
|[[:human_genes:R:RAN|RAN]]|2.30|0.37|
|[[:human_genes:A:AAAS|AAAS]]|2.33|0.50|
|[[:human_genes:P:PLA2G4E|PLA2G4E]]|2.35|0.50|
|[[:human_genes:N:NPAP1|NPAP1]]|2.35|0.55|
|[[:human_genes:S:SUPT6H|SUPT6H]]|2.35|0.63|
|[[:human_genes:A:ATP13A3|ATP13A3]]|2.37|0.37|
|[[:human_genes:D:DES|DES]]|2.38|0.37|
|[[:human_genes:A:AP2A1|AP2A1]]|2.39|0.50|
|[[:human_genes:T:TSPY1|TSPY1]]|2.42|0.37|
|[[:human_genes:A:ACO2|ACO2]]|2.42|0.63|
|[[:human_genes:S:STON2|STON2]]|2.43|0.37|
|[[:human_genes:P:PDE12|PDE12]]|2.52|0.37|
|[[:human_genes:U:UQCRH|UQCRH]]|2.52|0.50|
|[[:human_genes:I:IRX3|IRX3]]|2.53|0.37|
|[[:human_genes:A:ARRDC3|ARRDC3]]|2.54|0.37|
|[[:human_genes:A:ATXN1|ATXN1]]|2.56|0.50|
|[[:human_genes:C:CDC37L1|CDC37L1]]|2.57|0.50|
|[[:human_genes:Z:ZCCHC9|ZCCHC9]]|2.57|0.37|
|[[:human_genes:G:GNL2|GNL2]]|2.64|0.37|
|[[:human_genes:R:RAD51|RAD51]]|2.66|0.63|
|[[:human_genes:P:P2RY11|P2RY11]]|2.73|0.37|
|[[:human_genes:P:PSMD14|PSMD14]]|2.89|0.50|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp58|UM131593 0.1μM R01 exp58]]|0.056|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp49|NFN1 0.1μM R01 exp49]]|0.054|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp40|2-Methoxyestradiol 0.2μM R01 exp40]]|0.052|<button type="primary" size="small" modal="3">View</button>|
</modal>


<modal id="1" size="lg" title="Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53  vs  UM131593 0.1μM R01 exp58">
{{:chemogenomics:comparison_plots:exp53_vs_exp58.png?nolink |}}
</modal>

<modal id="2" size="lg" title="NFN1 0.1μM R01 exp49  vs  Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53">
{{:chemogenomics:comparison_plots:exp49_vs_exp53.png?nolink |}}
</modal>

<modal id="3" size="lg" title="2-Methoxyestradiol 0.2μM R01 exp40  vs  Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53">
{{:chemogenomics:comparison_plots:exp40_vs_exp53.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|proteasome regulatory particle|91.97|PSMD14,PSMD6,PSMD7|
|proteasome accessory complex|75.25|PSMD14,PSMD6,PSMD7|
|endopeptidase complex|26.59|PSMD14,PSMD6,PSMB1,PSMD7|
|nuclear pore|23.99|NPAP1,AAAS,RAN,NUP155|
|RNA transport|19.71|SUPT6H,NPAP1,AAAS,RAN,NUP155|
|nucleic acid transport|19.71|SUPT6H,NPAP1,AAAS,RAN,NUP155|
|establishment of RNA localization|19.43|SUPT6H,NPAP1,AAAS,RAN,NUP155|
|RNA localization|17.03|SUPT6H,NPAP1,AAAS,RAN,NUP155|
</modal>