AGK [The Human Chemical-Genetic Interaction Map Project]

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======= AGK =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: AGK
  * **<color #00a2e8>Official Name</color>**: acylglycerol kinase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=55750|55750]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q53H12|Q53H12]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=AGK&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20AGK|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/610345|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10. [provided by RefSeq, Feb 2012]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively (PubMed:15939762). Does not phosphorylate sphingosine (PubMed:15939762). Independently of its lipid kinase activity, acts as a component of the TIM22 complex (PubMed:28712724, PubMed:28712726). The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force (PubMed:28712724, PubMed:28712726). In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochtondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23 (PubMed:28712724). Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth (PubMed:15939762). {ECO:0000269|PubMed:15939762, ECO:0000269|PubMed:28712724, ECO:0000269|PubMed:28712726}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DAGK cat|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|acylglycerol kinase activity|
|ceramide kinase activity|
|TIM22 mitochondrial import inner membrane insertion complex|
|diacylglycerol kinase activity|
|protein insertion into mitochondrial inner membrane|
|establishment of protein localization to mitochondrial membrane|
|NAD+ kinase activity|
|protein insertion into mitochondrial membrane|
|integral component of mitochondrial inner membrane|
|protein insertion into membrane|
|inner mitochondrial membrane organization|
|ceramide biosynthetic process|
|protein targeting to mitochondrion|
|lipid phosphorylation|
|establishment of protein localization to mitochondrion|
|protein localization to mitochondrion|
|mitochondrial intermembrane space|
|ceramide metabolic process|
|sphingolipid biosynthetic process|
|mitochondrial membrane|
|mitochondrial membrane organization|
|membrane lipid biosynthetic process|
|sphingolipid metabolic process|
|mitochondrial outer membrane|
|lipid modification|
|membrane lipid metabolic process|
|glycerophospholipid biosynthetic process|
|mitochondrial transport|
|glycerolipid biosynthetic process|
|phospholipid biosynthetic process|
|establishment of protein localization to membrane|
|glycerophospholipid metabolic process|
|protein targeting|
|phospholipid metabolic process|
|glycerolipid metabolic process|
|mitochondrial inner membrane|
|establishment of protein localization to organelle|
|mitochondrion organization|
|protein localization to membrane|
|amide biosynthetic process|
|organophosphate biosynthetic process|
|lipid biosynthetic process|
|intracellular membrane-bounded organelle|
|protein localization to organelle|
|cellular amide metabolic process|
|membrane organization|
|organophosphate metabolic process|
|cellular lipid metabolic process|
|intracellular protein transport|
|lipid metabolic process|
|mitochondrion|
|phosphorylation|
|organonitrogen compound biosynthetic process|
|ATP binding|
|protein transport|
|intracellular transport|
|peptide transport|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|cellular nitrogen compound biosynthetic process|
|establishment of localization in cell|
|nitrogen compound transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp517|Quercetin 20μM R08 exp517]]|-1.84|
|[[:results:exp180|Dynasore 10μM R04 exp180]]|-1.77|
|[[:results:exp65|Mubritinib 0.2μM R02 exp65]]|1.82|
|[[:results:exp447|Amiloride 100μM R08 exp447]]|1.89|
|[[:results:exp11|CCCP 1μM R00 exp11]]|1.89|
|[[:results:exp274|Citral 50μM R06 exp274]]|2.01|
|[[:results:exp431|Rotenone 0.07μM R08 exp431]]|2.06|
|[[:results:exp13|Chloramphenicol 20μM R00 exp13]]|2.08|
|[[:results:exp439|QNZ 0.01μM R08 exp439]]|2.13|
|[[:results:exp12|Chloramphenicol 2μM R00 exp12]]|2.54|
|[[:results:exp209|Deguelin 0.15μM R05 exp209]]|2.74|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 16440
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.52 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='AGK Expression in NALM6 Cells: 4.52'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>