AKAP8 [The Human Chemical-Genetic Interaction Map Project]

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======= AKAP8 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: AKAP8
  * **<color #00a2e8>Official Name</color>**: A-kinase anchoring protein 8
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=10270|10270]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O43823|O43823]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=AKAP8&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20AKAP8|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/604692|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II) (PubMed:9473338). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring (PubMed:10601332, PubMed:10791967, PubMed:11964380). May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression (PubMed:14641107). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function may act redundantly with AKAP8L (PubMed:16980585). Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation (PubMed:22130794). May be involved in regulation of DNA replication by acting as scaffold for MCM2 (PubMed:12740381). Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation (PubMed:23995757). May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells (PubMed:16227597). May act as a carrier protein of GJA1 for its transport to the nucleus (PubMed:26880274). Seems to involved in modulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro and associates to GC-rich ribosomal RNA promoters (PubMed:26683827). Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (By similarity). {ECO:0000250|UniProtKB:Q63014, ECO:0000250|UniProtKB:Q9DBR0, ECO:0000269|PubMed:10601332, ECO:0000269|PubMed:10791967, ECO:0000269|PubMed:11964380, ECO:0000269|PubMed:16980585, ECO:0000269|PubMed:22130794, ECO:0000269|PubMed:26683827, ECO:0000269|PubMed:26880274, ECO:0000305|PubMed:14641107, ECO:0000305|PubMed:9473338}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|AKAP95|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|female pronucleus|
|regulation of histone phosphorylation|
|cellular response to prostaglandin E stimulus|
|mitotic chromosome condensation|
|positive regulation of histone deacetylation|
|protein kinase A regulatory subunit binding|
|positive regulation of protein deacetylation|
|cellular response to prostaglandin stimulus|
|response to prostaglandin E|
|condensed chromosome|
|regulation of histone deacetylation|
|NF-kappaB binding|
|response to prostaglandin|
|regulation of protein deacetylation|
|chromosome condensation|
|cellular response to fatty acid|
|negative regulation of tumor necrosis factor production|
|negative regulation of tumor necrosis factor superfamily cytokine production|
|cellular response to alcohol|
|response to fatty acid|
|positive regulation of histone modification|
|cellular response to ketone|
|double-stranded DNA binding|
|positive regulation of chromatin organization|
|mitotic sister chromatid segregation|
|nuclear matrix|
|histone deacetylase binding|
|cell cycle G2/M phase transition|
|sister chromatid segregation|
|mitotic nuclear division|
|regulation of histone modification|
|regulation of tumor necrosis factor production|
|regulation of tumor necrosis factor superfamily cytokine production|
|positive regulation of chromosome organization|
|DNA packaging|
|cellular response to lipopolysaccharide|
|regulation of chromatin organization|
|cellular response to molecule of bacterial origin|
|response to ketone|
|cellular response to acid chemical|
|cellular response to biotic stimulus|
|nuclear chromosome segregation|
|response to alcohol|
|negative regulation of cytokine production|
|chromosome segregation|
|cell cycle phase transition|
|nuclear division|
|DNA conformation change|
|organelle fission|
|response to lipopolysaccharide|
|response to molecule of bacterial origin|
|response to acid chemical|
|regulation of chromosome organization|
|cellular response to lipid|
|mitotic cell cycle process|
|cellular response to hormone stimulus|
|positive regulation of organelle organization|
|mitotic cell cycle|
|response to bacterium|
|regulation of cytokine production|
|innate immune response|
|zinc ion binding|
|response to lipid|
|nucleolus|
|response to hormone|
|defense response to other organism|
|Golgi apparatus|
|cell cycle process|
|cellular response to oxygen-containing compound|
|chromosome organization|
|negative regulation of multicellular organismal process|
|positive regulation of cellular component organization|
|cellular response to endogenous stimulus|
|positive regulation of protein modification process|
|mitochondrion|
|regulation of organelle organization|
|response to other organism|
|response to external biotic stimulus|
|response to biotic stimulus|
|defense response|
|cell cycle|
|RNA binding|
|regulation of protein phosphorylation|
|response to endogenous stimulus|
|protein transport|
|peptide transport|
|response to oxygen-containing compound|
|amide transport|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|establishment of protein localization|
|positive regulation of protein metabolic process|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|nitrogen compound transport|
|regulation of protein modification process|
|immune response|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|-1.77|
|[[:results:exp435|JQ1 0.8μM R08 exp435]]|1.77|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 9508
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.43 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='AKAP8 Expression in NALM6 Cells: 6.43'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>