AP2A1 [The Human Chemical-Genetic Interaction Map Project]

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======= AP2A1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: AP2A1
  * **<color #00a2e8>Official Name</color>**: adaptor related protein complex 2 subunit alpha 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=160|160]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O95782|O95782]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=AP2A1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20AP2A1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/601026|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C- terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity). {ECO:0000250, ECO:0000269|PubMed:14745134, ECO:0000269|PubMed:15473838, ECO:0000269|PubMed:19033387}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Alpha adaptinC2|
|Adaptin N|
|Alpha adaptin C|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|negative regulation of hyaluronan biosynthetic process|
|clathrin coat of trans-Golgi network vesicle|
|regulation of hyaluronan biosynthetic process|
|clathrin adaptor activity|
|low-density lipoprotein particle receptor catabolic process|
|low-density lipoprotein receptor particle metabolic process|
|endolysosome membrane|
|AP-2 adaptor complex|
|filopodium tip|
|Golgi to endosome transport|
|low-density lipoprotein particle receptor binding|
|low-density lipoprotein particle clearance|
|clathrin-dependent endocytosis|
|clathrin-coated endocytic vesicle|
|receptor catabolic process|
|regulation of defense response to virus by virus|
|clathrin-coated endocytic vesicle membrane|
|regulation of polysaccharide biosynthetic process|
|plasma lipoprotein particle clearance|
|regulation of polysaccharide metabolic process|
|negative regulation of carbohydrate metabolic process|
|positive regulation of receptor-mediated endocytosis|
|endocytic vesicle membrane|
|regulation of plasma lipoprotein particle levels|
|ephrin receptor signaling pathway|
|negative regulation of small molecule metabolic process|
|regulation of carbohydrate biosynthetic process|
|Wnt signaling pathway, planar cell polarity pathway|
|antigen processing and presentation of exogenous peptide antigen via MHC class II|
|positive regulation of endocytosis|
|regulation of receptor-mediated endocytosis|
|antigen processing and presentation of peptide or polysaccharide antigen via MHC class II|
|antigen processing and presentation of peptide antigen via MHC class II|
|post-Golgi vesicle-mediated transport|
|receptor metabolic process|
|regulation of establishment of planar polarity|
|non-canonical Wnt signaling pathway|
|cytosolic transport|
|antigen processing and presentation of exogenous peptide antigen|
|regulation of morphogenesis of an epithelium|
|antigen processing and presentation of exogenous antigen|
|protein C-terminus binding|
|antigen processing and presentation of peptide antigen|
|basolateral plasma membrane|
|regulation of carbohydrate metabolic process|
|regulation of endocytosis|
|antigen processing and presentation|
|receptor-mediated endocytosis|
|regulation of animal organ morphogenesis|
|protein-containing complex binding|
|positive regulation of neuron projection development|
|apical plasma membrane|
|Wnt signaling pathway|
|cell-cell signaling by wnt|
|positive regulation of neuron differentiation|
|Golgi vesicle transport|
|positive regulation of cell projection organization|
|regulation of small molecule metabolic process|
|cell surface receptor signaling pathway involved in cell-cell signaling|
|protein kinase binding|
|positive regulation of neurogenesis|
|regulation of neuron projection development|
|transmembrane receptor protein tyrosine kinase signaling pathway|
|positive regulation of nervous system development|
|positive regulation of cell development|
|regulation of vesicle-mediated transport|
|endocytosis|
|cellular protein catabolic process|
|regulation of neuron differentiation|
|import into cell|
|protein catabolic process|
|regulation of plasma membrane bounded cell projection organization|
|viral process|
|regulation of cell projection organization|
|enzyme linked receptor protein signaling pathway|
|symbiotic process|
|regulation of neurogenesis|
|interspecies interaction between organisms|
|membrane organization|
|cellular macromolecule catabolic process|
|regulation of nervous system development|
|regulation of cell development|
|positive regulation of cell differentiation|
|positive regulation of transport|
|intracellular protein transport|
|macromolecule catabolic process|
|regulation of anatomical structure morphogenesis|
|organonitrogen compound catabolic process|
|cell-cell signaling|
|positive regulation of cellular component organization|
|positive regulation of developmental process|
|negative regulation of macromolecule biosynthetic process|
|protein transport|
|negative regulation of cellular biosynthetic process|
|intracellular transport|
|generation of neurons|
|peptide transport|
|negative regulation of biosynthetic process|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|neurogenesis|
|positive regulation of multicellular organismal process|
|organic substance catabolic process|
|cellular catabolic process|
|regulation of cell differentiation|
|establishment of localization in cell|
|nitrogen compound transport|
|regulation of transport|
|vesicle-mediated transport|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|-2.26|
|[[:results:exp122|Golgicide-A 4μM R03 exp122]]|-2.14|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|-2.11|
|[[:results:exp346|CoCl2 18μM R07 exp346]]|-1.88|
|[[:results:exp244|SB743921 0.001μM R05 exp244]]|-1.85|
|[[:results:exp66|BI-D1870 3.15μM R02 exp66]]|-1.73|
|[[:results:exp227|Cryptotanshinone 12μM R05 exp227]]|-1.71|
|[[:results:exp53|Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53]]|2.39|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 8731
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.12 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='AP2A1 Expression in NALM6 Cells: 6.12'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>