BUB1 [The Human Chemical-Genetic Interaction Map Project]

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======= BUB1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: BUB1
  * **<color #00a2e8>Official Name</color>**: BUB1 mitotic checkpoint serine/threonine kinase
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=699|699]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O43683|O43683]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=BUB1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20BUB1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/602452|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis. {ECO:0000269|PubMed:10198256, ECO:0000269|PubMed:15020684, ECO:0000269|PubMed:15525512, ECO:0000269|PubMed:15723797, ECO:0000269|PubMed:16760428, ECO:0000269|PubMed:17158872, ECO:0000269|PubMed:19487456, ECO:0000269|PubMed:20739936}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Pkinase Tyr|
|Mad3 BUB1 I|
|Pkinase|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|condensed nuclear chromosome outer kinetochore|
|meiotic sister chromatid cohesion, centromeric|
|centromeric sister chromatid cohesion|
|meiotic sister chromatid cohesion|
|meiotic sister chromatid segregation|
|condensed nuclear chromosome kinetochore|
|meiosis II|
|meiosis II cell cycle process|
|regulation of sister chromatid cohesion|
|mitotic spindle assembly checkpoint|
|spindle checkpoint|
|mitotic spindle checkpoint|
|spindle assembly checkpoint|
|negative regulation of mitotic metaphase/anaphase transition|
|negative regulation of metaphase/anaphase transition of cell cycle|
|negative regulation of mitotic sister chromatid separation|
|negative regulation of chromosome separation|
|negative regulation of mitotic sister chromatid segregation|
|negative regulation of sister chromatid segregation|
|negative regulation of chromosome segregation|
|negative regulation of mitotic nuclear division|
|negative regulation of nuclear division|
|sister chromatid cohesion|
|regulation of mitotic metaphase/anaphase transition|
|regulation of metaphase/anaphase transition of cell cycle|
|regulation of mitotic sister chromatid separation|
|regulation of chromosome separation|
|chromosome organization involved in meiotic cell cycle|
|regulation of mitotic sister chromatid segregation|
|regulation of sister chromatid segregation|
|kinetochore|
|condensed chromosome kinetochore|
|meiotic chromosome segregation|
|regulation of chromosome segregation|
|negative regulation of chromosome organization|
|sister chromatid segregation|
|meiotic nuclear division|
|mitotic cell cycle checkpoint|
|meiotic cell cycle process|
|regulation of mitotic nuclear division|
|regulation of nuclear division|
|cell cycle checkpoint|
|negative regulation of mitotic cell cycle phase transition|
|nuclear chromosome segregation|
|meiotic cell cycle|
|negative regulation of cell cycle phase transition|
|protein kinase activity|
|chromosome segregation|
|nuclear division|
|negative regulation of mitotic cell cycle|
|organelle fission|
|negative regulation of cell cycle process|
|regulation of chromosome organization|
|protein serine/threonine kinase activity|
|negative regulation of organelle organization|
|regulation of mitotic cell cycle phase transition|
|regulation of cell cycle phase transition|
|cell division|
|cell population proliferation|
|negative regulation of cell cycle|
|mitotic cell cycle process|
|regulation of mitotic cell cycle|
|mitotic cell cycle|
|viral process|
|negative regulation of cellular component organization|
|regulation of cell cycle process|
|symbiotic process|
|interspecies interaction between organisms|
|apoptotic process|
|protein phosphorylation|
|cell cycle process|
|programmed cell death|
|chromosome organization|
|cell death|
|regulation of cell cycle|
|phosphorylation|
|regulation of organelle organization|
|cell cycle|
|reproductive process|
|reproduction|
|ATP binding|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp97|BI-6727 0.0125μM R03 exp97]]|-2.46|
|[[:results:exp95|BI-2536 0.0042μM R03 exp95]]|-2.2|
|[[:results:exp128|GSK591 2.6μM R03 exp128]]|-1.97|
|[[:results:exp269|Bisphenol A 100μM R06 exp269]]|-1.88|
|[[:results:exp47|Lapatinib 5μM R01 exp47]]|-1.72|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|-1.71|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|-1.7|
|[[:results:exp285|GW501516 25μM R06 exp285]]|2.01|
|[[:results:exp244|SB743921 0.001μM R05 exp244]]|2.15|
|[[:results:exp33|Rotenone 2μM R00 exp33]]|2.42|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hars|HARS]]|0.519|
|[[:human genes:k:kntc1|KNTC1]]|0.442|
|[[:human genes:g:gak|GAK]]|0.403|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 23/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|1/13|
|fibroblast|0/1|
|gastric|1/15|
|kidney|3/21|
|liver|1/20|
|lung|2/75|
|lymphocyte|0/14|
|ovary|1/26|
|pancreas|0/24|
|peripheral nervous system|1/16|
|plasma cell|1/15|
|prostate|0/1|
|skin|1/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|1/22|
|urinary tract|1/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2958
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.14 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='BUB1 Expression in NALM6 Cells: 8.14'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>