CENPE [The Human Chemical-Genetic Interaction Map Project]

This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong.
======= CENPE =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: CENPE
  * **<color #00a2e8>Official Name</color>**: centromere protein E
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=1062|1062]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q02224|Q02224]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=CENPE&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20CENPE|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/117143|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule- kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules (PubMed:7889940, PubMed:23891108, PubMed:25395579). The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated (PubMed:25908662). Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends (PubMed:23955301). Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized (PubMed:25743205). Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules (PubMed:17535814) The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss (By similarity). {ECO:0000250|UniProtKB:Q6RT24, ECO:0000269|PubMed:17535814, ECO:0000269|PubMed:23891108, ECO:0000269|PubMed:23955301, ECO:0000269|PubMed:25395579, ECO:0000269|PubMed:25743205, ECO:0000269|PubMed:25908662, ECO:0000269|PubMed:7889940}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Kinesin|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|lateral attachment of mitotic spindle microtubules to kinetochore|
|microtubule plus-end directed mitotic chromosome migration|
|mitotic chromosome movement towards spindle pole|
|kinetochore binding|
|kinetochore microtubule|
|chromosome movement towards spindle pole|
|condensed chromosome, centromeric region|
|attachment of mitotic spindle microtubules to kinetochore|
|mitotic spindle midzone|
|kinetochore assembly|
|kinetochore organization|
|attachment of spindle microtubules to kinetochore|
|spindle midzone|
|centromere complex assembly|
|mitotic metaphase plate congression|
|regulation of mitotic metaphase/anaphase transition|
|regulation of metaphase/anaphase transition of cell cycle|
|kinesin complex|
|chromosome, centromeric region|
|metaphase plate congression|
|regulation of mitotic sister chromatid separation|
|regulation of chromosome separation|
|microtubule motor activity|
|regulation of mitotic sister chromatid segregation|
|establishment of chromosome localization|
|chromosome localization|
|mitotic spindle organization|
|regulation of sister chromatid segregation|
|retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum|
|kinetochore|
|condensed chromosome kinetochore|
|antigen processing and presentation of exogenous peptide antigen via MHC class II|
|antigen processing and presentation of peptide antigen via MHC class II|
|antigen processing and presentation of peptide or polysaccharide antigen via MHC class II|
|regulation of chromosome segregation|
|microtubule cytoskeleton organization involved in mitosis|
|mitotic sister chromatid segregation|
|chromosome|
|sister chromatid segregation|
|mitotic nuclear division|
|microtubule cytoskeleton|
|spindle organization|
|midbody|
|regulation of mitotic nuclear division|
|antigen processing and presentation of exogenous peptide antigen|
|antigen processing and presentation of exogenous antigen|
|antigen processing and presentation of peptide antigen|
|regulation of nuclear division|
|protein-DNA complex assembly|
|antigen processing and presentation|
|nuclear chromosome segregation|
|ATPase activity|
|microtubule binding|
|protein-DNA complex subunit organization|
|microtubule-based movement|
|chromosome segregation|
|nuclear division|
|organelle fission|
|microtubule|
|regulation of chromosome organization|
|establishment of organelle localization|
|Golgi vesicle transport|
|regulation of mitotic cell cycle phase transition|
|regulation of cell cycle phase transition|
|microtubule cytoskeleton organization|
|cell division|
|positive regulation of protein kinase activity|
|positive regulation of kinase activity|
|organelle localization|
|mitotic cell cycle process|
|regulation of mitotic cell cycle|
|positive regulation of transferase activity|
|microtubule-based process|
|mitotic cell cycle|
|regulation of cell cycle process|
|organelle assembly|
|regulation of protein kinase activity|
|cellular protein-containing complex assembly|
|regulation of kinase activity|
|regulation of transferase activity|
|cell cycle process|
|positive regulation of protein phosphorylation|
|positive regulation of phosphorylation|
|chromosome organization|
|cytoskeleton organization|
|positive regulation of phosphate metabolic process|
|positive regulation of phosphorus metabolic process|
|regulation of cell cycle|
|positive regulation of protein modification process|
|regulation of organelle organization|
|cell cycle|
|positive regulation of catalytic activity|
|regulation of protein phosphorylation|
|ATP binding|
|movement of cell or subcellular component|
|protein-containing complex assembly|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|positive regulation of protein metabolic process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|establishment of localization in cell|
|regulation of protein modification process|
|protein-containing complex subunit organization|
|vesicle-mediated transport|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|-2.38|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|-2.01|
|[[:results:exp217|Mdivi-1 15μM R05 exp217]]|-1.74|
|[[:results:exp153|SGC2096 2.6μM R03 exp153]]|1.83|
|[[:results:exp93|DABN racemic mixture R03 exp93]]|1.86|
|[[:results:exp292|Menadione 5μM R06 exp292]]|1.87|
|[[:results:exp27|Pimelic-diphenylamide-106 0.5μM R00 exp27]]|1.89|
|[[:results:exp42|BI-6727 0.001μM R01 exp42]]|1.93|
|[[:results:exp94|Nocodazole 0.1μM R03 exp94]]|2.5|
|[[:results:exp33|Rotenone 2μM R00 exp33]]|3.68|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:z:zw10|ZW10]]|0.458|
|[[:human genes:k:kntc1|KNTC1]]|0.438|
|[[:human genes:t:trip13|TRIP13]]|0.437|
|[[:human genes:m:mad1l1|MAD1L1]]|0.421|
|[[:human genes:m:mad2l1bp|MAD2L1BP]]|0.416|
|[[:human genes:z:zwilch|ZWILCH]]|0.415|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 27/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|1/28|
|bone|0/26|
|breast|0/33|
|central nervous system|4/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|1/16|
|kidney|3/21|
|liver|1/20|
|lung|3/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|1/16|
|plasma cell|1/15|
|prostate|0/1|
|skin|1/24|
|soft tissue|1/9|
|thyroid|0/2|
|upper aerodigestive|1/22|
|urinary tract|1/29|
|uterus|2/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1489
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.01 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='CENPE Expression in NALM6 Cells: 8.01'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>