CPSF3 [The Human Chemical-Genetic Interaction Map Project]

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======= CPSF3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: CPSF3
  * **<color #00a2e8>Official Name</color>**: cleavage and polyadenylation specific factor 3
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=51692|51692]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9UKF6|Q9UKF6]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=CPSF3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20CPSF3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/606029|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. {ECO:0000269|PubMed:14749727, ECO:0000269|PubMed:15037765, ECO:0000269|PubMed:17128255, ECO:0000269|PubMed:18688255}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Beta-Casp|
|RMMBL|
|Lactamase B 2|
|CPSF73-100 C|
|Lactamase B|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|mRNA 3-end processing by stem-loop binding and cleavage|
|5-3 exonuclease activity|
|mRNA cleavage and polyadenylation specificity factor complex|
|mRNA cleavage|
|endoribonuclease activity|
|mRNA polyadenylation|
|histone mRNA metabolic process|
|RNA polyadenylation|
|termination of RNA polymerase II transcription|
|DNA-templated transcription, termination|
|RNA phosphodiester bond hydrolysis, endonucleolytic|
|mRNA 3-end processing|
|mRNA export from nucleus|
|mRNA-containing ribonucleoprotein complex export from nucleus|
|ribonucleoprotein complex export from nucleus|
|ribonucleoprotein complex localization|
|RNA export from nucleus|
|RNA 3-end processing|
|protein export from nucleus|
|mRNA transport|
|nuclear export|
|RNA phosphodiester bond hydrolysis|
|nucleic acid transport|
|RNA transport|
|establishment of RNA localization|
|RNA localization|
|nucleobase-containing compound transport|
|protein-containing complex localization|
|nucleocytoplasmic transport|
|nuclear transport|
|mRNA splicing, via spliceosome|
|RNA splicing, via transesterification reactions with bulged adenosine as nucleophile|
|nucleic acid phosphodiester bond hydrolysis|
|RNA splicing, via transesterification reactions|
|RNA splicing|
|mRNA processing|
|transcription by RNA polymerase II|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|RNA biosynthetic process|
|mRNA metabolic process|
|RNA processing|
|intracellular protein transport|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|organic cyclic compound biosynthetic process|
|RNA binding|
|protein transport|
|intracellular transport|
|peptide transport|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|cellular nitrogen compound biosynthetic process|
|RNA metabolic process|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|establishment of localization in cell|
|nitrogen compound transport|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|-1.95|
|[[:results:exp292|Menadione 5μM R06 exp292]]|1.72|
|[[:results:exp61|YM155 0.0002μM R01 exp61]]|1.77|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hgc6.3|HGC6.3]]|0.437|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 492/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|20/28|
|blood|18/28|
|bone|20/25|
|breast|20/33|
|central nervous system|43/56|
|cervix|4/4|
|colorectal|11/17|
|esophagus|11/13|
|fibroblast|1/1|
|gastric|10/15|
|kidney|14/21|
|liver|11/20|
|lung|52/75|
|lymphocyte|13/14|
|ovary|16/26|
|pancreas|18/24|
|peripheral nervous system|14/16|
|plasma cell|12/15|
|prostate|1/1|
|skin|13/24|
|soft tissue|5/7|
|thyroid|2/2|
|upper aerodigestive|15/22|
|urinary tract|17/29|
|uterus|4/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 211
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.46 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='CPSF3 Expression in NALM6 Cells: 6.46'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>