DDB2 [The Human Chemical-Genetic Interaction Map Project]

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======= DDB2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: DDB2
  * **<color #00a2e8>Official Name</color>**: damage specific DNA binding protein 2
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=1643|1643]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q92466|Q92466]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=DDB2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20DDB2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/600811|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Required for DNA repair. Binds to DDB1 to form the UV- damaged DNA-binding protein complex (the UV-DDB complex). The UV- DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1- CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4- ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair. {ECO:0000269|PubMed:10882109, ECO:0000269|PubMed:11278856, ECO:0000269|PubMed:11705987, ECO:0000269|PubMed:12732143, ECO:0000269|PubMed:12944386, ECO:0000269|PubMed:14751237, ECO:0000269|PubMed:15882621, ECO:0000269|PubMed:16260596, ECO:0000269|PubMed:16473935, ECO:0000269|PubMed:16678110, ECO:0000269|PubMed:18593899, ECO:0000269|PubMed:9892649}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|WD40|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|Cul4B-RING E3 ubiquitin ligase complex|
|pyrimidine dimer repair|
|UV-damage excision repair|
|histone H2A monoubiquitination|
|nucleotide-excision repair, preincision complex stabilization|
|nucleotide-excision repair, DNA duplex unwinding|
|nucleotide-excision repair, DNA incision, 3-to lesion|
|nucleotide-excision repair, DNA damage recognition|
|histone H2A ubiquitination|
|global genome nucleotide-excision repair|
|Cul4-RING E3 ubiquitin ligase complex|
|nucleotide-excision repair, preincision complex assembly|
|histone monoubiquitination|
|nucleotide-excision repair, DNA incision, 5-to lesion|
|nucleotide-excision repair, DNA incision|
|histone ubiquitination|
|damaged DNA binding|
|protein monoubiquitination|
|protein autoubiquitination|
|cellular response to UV|
|nucleotide-excision repair|
|DNA duplex unwinding|
|cellular response to light stimulus|
|DNA geometric change|
|response to UV|
|cellular response to radiation|
|protein-DNA complex assembly|
|protein-DNA complex subunit organization|
|ubiquitin-protein transferase activity|
|positive regulation of protein complex assembly|
|protein-containing complex binding|
|protein deubiquitination|
|DNA conformation change|
|protein modification by small protein removal|
|nucleic acid phosphodiester bond hydrolysis|
|protein polyubiquitination|
|response to light stimulus|
|cellular response to abiotic stimulus|
|cellular response to environmental stimulus|
|post-translational protein modification|
|histone modification|
|covalent chromatin modification|
|response to radiation|
|regulation of protein complex assembly|
|DNA repair|
|positive regulation of cellular component biogenesis|
|cell junction|
|protein-containing complex|
|protein ubiquitination|
|chromatin organization|
|DNA metabolic process|
|protein modification by small protein conjugation|
|cellular response to DNA damage stimulus|
|cellular protein-containing complex assembly|
|regulation of cellular component biogenesis|
|protein modification by small protein conjugation or removal|
|chromosome organization|
|response to abiotic stimulus|
|positive regulation of cellular component organization|
|proteolysis|
|DNA binding|
|protein-containing complex assembly|
|cellular response to stress|
|protein-containing complex subunit organization|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|1.88|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 11659
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.34 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='DDB2 Expression in NALM6 Cells: 6.34'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>