DDOST [The Human Chemical-Genetic Interaction Map Project]

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======= DDOST =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: DDOST
  * **<color #00a2e8>Official Name</color>**: dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=1650|1650]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P39656|P39656]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=DDOST&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20DDOST|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/602202|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Required for the assembly of both SST3A- and SS3B-containing OST complexes. Required for efficient N-glycosylation. {ECO:0000250|UniProtKB:Q05052, ECO:0000269|PubMed:22467853, ECO:0000305}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DDOST 48kD|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|oligosaccharyl transferase activity|
|dolichyl-diphosphooligosaccharide-protein glycotransferase activity|
|oligosaccharyltransferase complex|
|protein N-linked glycosylation via asparagine|
|peptidyl-asparagine modification|
|azurophil granule membrane|
|protein N-linked glycosylation|
|T cell activation|
|protein glycosylation|
|macromolecule glycosylation|
|glycosylation|
|glycoprotein biosynthetic process|
|lymphocyte activation|
|glycoprotein metabolic process|
|neutrophil degranulation|
|neutrophil activation involved in immune response|
|neutrophil mediated immunity|
|neutrophil activation|
|granulocyte activation|
|leukocyte degranulation|
|myeloid leukocyte mediated immunity|
|myeloid cell activation involved in immune response|
|myeloid leukocyte activation|
|protein-containing complex|
|carbohydrate derivative biosynthetic process|
|leukocyte activation involved in immune response|
|cell activation involved in immune response|
|intracellular membrane-bounded organelle|
|regulated exocytosis|
|leukocyte mediated immunity|
|exocytosis|
|peptidyl-amino acid modification|
|leukocyte activation|
|endoplasmic reticulum membrane|
|secretion by cell|
|endoplasmic reticulum|
|carbohydrate derivative metabolic process|
|export from cell|
|cell activation|
|immune effector process|
|response to cytokine|
|secretion|
|organonitrogen compound biosynthetic process|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|immune response|
|vesicle-mediated transport|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp112|S-DABN 8μM R03 exp112]]|-2.14|
|[[:results:exp494|Isoniazid 100μM R08 exp494]]|-2.01|
|[[:results:exp31|Rifampicin 1μM R00 exp31]]|-1.8|
|[[:results:exp6|Bortezomib 0.005μM R00 exp6]]|-1.75|
|[[:results:exp18|Doxycycline 10μM R00 exp18]]|-1.7|
|[[:results:exp531|THZ1 0.06μM R08 exp531]]|1.73|
|[[:results:exp22|MLN-4924 2μM R00 exp22]]|1.77|
|[[:results:exp96|BI-2536 0.02μM R03 exp96]]|1.8|
|[[:results:exp93|DABN racemic mixture R03 exp93]]|1.96|
|[[:results:exp126|GSK461364A 0.1μM R03 exp126]]|2.06|
|[[:results:exp443|SNS-032 15μM R08 exp443]]|2.43|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:r:rpn1|RPN1]]|0.602|
|[[:human genes:g:gmppb|GMPPB]]|0.584|
|[[:human genes:h:hars|HARS]]|0.577|
|[[:human genes:p:pmm2|PMM2]]|0.541|
|[[:human genes:d:dad1|DAD1]]|0.53|
|[[:human genes:t:thg1l|THG1L]]|0.51|
|[[:human genes:a:alg2|ALG2]]|0.51|
|[[:human genes:r:rnmt|RNMT]]|0.509|
|[[:human genes:e:exoc4|EXOC4]]|0.507|
|[[:human genes:o:osgep|OSGEP]]|0.507|
|[[:human genes:g:gnb1l|GNB1L]]|0.505|
|[[:human genes:t:tti2|TTI2]]|0.503|
|[[:human genes:a:alg1|ALG1]]|0.503|
|[[:human genes:p:pam16|PAM16]]|0.5|
|[[:human genes:m:med16|MED16]]|0.499|
|[[:human genes:e:eprs|EPRS]]|0.496|
|[[:human genes:r:rtcb|RTCB]]|0.488|
|[[:human genes:p:pelo|PELO]]|0.487|
|[[:human genes:a:aars|AARS]]|0.487|
|[[:human genes:d:dhps|DHPS]]|0.487|
|[[:human genes:r:rptor|RPTOR]]|0.481|
|[[:human genes:e:exoc3|EXOC3]]|0.48|
|[[:human genes:d:dpagt1|DPAGT1]]|0.476|
|[[:human genes:s:srp14|SRP14]]|0.467|
|[[:human genes:t:triap1|TRIAP1]]|0.466|
|[[:human genes:s:spcs2|SPCS2]]|0.464|
|[[:human genes:r:rpn2|RPN2]]|0.463|
|[[:human genes:t:tmem258|TMEM258]]|0.461|
|[[:human genes:a:alg13|ALG13]]|0.459|
|[[:human genes:o:oraov1|ORAOV1]]|0.458|
|[[:human genes:c:ccdc101|CCDC101]]|0.454|
|[[:human genes:t:tsen54|TSEN54]]|0.452|
|[[:human genes:n:nae1|NAE1]]|0.45|
|[[:human genes:a:alg14|ALG14]]|0.447|
|[[:human genes:u:uap1|UAP1]]|0.446|
|[[:human genes:g:gfpt1|GFPT1]]|0.446|
|[[:human genes:c:crcp|CRCP]]|0.444|
|[[:human genes:d:dohh|DOHH]]|0.443|
|[[:human genes:o:ost4|OST4]]|0.441|
|[[:human genes:p:pisd|PISD]]|0.441|
|[[:human genes:d:ddx20|DDX20]]|0.439|
|[[:human genes:g:gtf3c3|GTF3C3]]|0.438|
|[[:human genes:m:mars|MARS]]|0.433|
|[[:human genes:r:rpp21|RPP21]]|0.433|
|[[:human genes:w:wdr24|WDR24]]|0.427|
|[[:human genes:n:nsmce1|NSMCE1]]|0.427|
|[[:human genes:h:hspd1|HSPD1]]|0.426|
|[[:human genes:g:gtf3c4|GTF3C4]]|0.426|
|[[:human genes:m:mtor|MTOR]]|0.425|
|[[:human genes:z:znf259|ZNF259]]|0.425|
|[[:human genes:m:max|MAX]]|0.422|
|[[:human genes:e:eif4e|EIF4E]]|0.421|
|[[:human genes:t:tti1|TTI1]]|0.416|
|[[:human genes:r:romo1|ROMO1]]|0.415|
|[[:human genes:y:yars|YARS]]|0.413|
|[[:human genes:m:meaf6|MEAF6]]|0.412|
|[[:human genes:p:ppp2r3c|PPP2R3C]]|0.409|
|[[:human genes:d:dr1|DR1]]|0.408|
|[[:human genes:a:alg5|ALG5]]|0.407|
|[[:human genes:g:gtf3c2|GTF3C2]]|0.401|
|[[:human genes:g:gpn2|GPN2]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 338/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|0/1|
|bile duct|17/28|
|blood|9/28|
|bone|7/26|
|breast|13/33|
|central nervous system|28/56|
|cervix|4/4|
|colorectal|13/17|
|esophagus|3/13|
|fibroblast|0/1|
|gastric|5/16|
|kidney|11/21|
|liver|12/20|
|lung|33/75|
|lymphocyte|3/16|
|ovary|10/26|
|pancreas|12/24|
|peripheral nervous system|9/16|
|plasma cell|11/15|
|prostate|1/1|
|skin|6/24|
|soft tissue|5/9|
|thyroid|1/2|
|upper aerodigestive|13/22|
|urinary tract|15/29|
|uterus|3/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 505
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.89 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='DDOST Expression in NALM6 Cells: 7.89'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>