DHX9 [The Human Chemical-Genetic Interaction Map Project]

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======= DHX9 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: DHX9
  * **<color #00a2e8>Official Name</color>**: DExH-box helicase 9
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=1660|1660]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q08211|Q08211]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=DHX9&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20DHX9|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603115|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.[provided by RefSeq, Feb 2010]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Multifunctional ATP-dependent nucleic acid helicase that unwinds DNA and RNA in a 3' to 5' direction and that plays important roles in many processes, such as DNA replication, transcriptional activation, post-transcriptional RNA regulation, mRNA translation and RNA-mediated gene silencing (PubMed:9111062, PubMed:11416126, PubMed:12711669, PubMed:15355351, PubMed:16680162, PubMed:17531811, PubMed:20669935, PubMed:21561811, PubMed:24049074, PubMed:25062910, PubMed:24990949, PubMed:28221134). Requires a 3'-single-stranded tail as entry site for acid nuclei unwinding activities as well as the binding and hydrolyzing of any of the four ribo- or deoxyribo- nucleotide triphosphates (NTPs) (PubMed:1537828). Unwinds numerous nucleic acid substrates such as double-stranded (ds) DNA and RNA, DNA:RNA hybrids, DNA and RNA forks composed of either partially complementary DNA duplexes or DNA:RNA hybrids, respectively, and also DNA and RNA displacement loops (D- and R-loops), triplex- helical DNA (H-DNA) structure and DNA and RNA-based G-quadruplexes (PubMed:20669935, PubMed:21561811, PubMed:24049074). Binds dsDNA, single-stranded DNA (ssDNA), dsRNA, ssRNA and poly(A)-containing RNA (PubMed:9111062, PubMed:10198287). Binds also to circular dsDNA or dsRNA of either linear and/or circular forms and stimulates the relaxation of supercoiled DNAs catalyzed by topoisomerase TOP2A (PubMed:12711669). Plays a role in DNA replication at origins of replication and cell cycle progression (PubMed:24990949). Plays a role as a transcriptional coactivator acting as a bridging factor between polymerase II holoenzyme and transcription factors or cofactors, such as BRCA1, CREBBP, RELA and SMN1 (PubMed:11149922, PubMed:9323138, PubMed:9662397, PubMed:11038348, PubMed:11416126, PubMed:15355351, PubMed:28221134). Binds to the CDKN2A promoter (PubMed:11038348). Plays several roles in post-transcriptional regulation of gene expression (PubMed:28221134, PubMed:28355180). In cooperation with NUP98, promotes pre-mRNA alternative splicing activities of a subset of genes (PubMed:11402034, PubMed:16680162, PubMed:28221134, PubMed:28355180). As component of a large PER complex, is involved in the negative regulation of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms (By similarity). Acts also as a nuclear resolvase that is able to bind and neutralize harmful massive secondary double-stranded RNA structures formed by inverted-repeat Alu retrotransposon elements that are inserted and transcribed as parts of genes during the process of gene transposition (PubMed:28355180). Involved in the positive regulation of nuclear export of constitutive transport element (CTE)-containing unspliced mRNA (PubMed:9162007, PubMed:10924507, PubMed:11402034). Component of the coding region determinant (CRD)-mediated complex that promotes cytoplasmic MYC mRNA stability (PubMed:19029303). Plays a role in mRNA translation (PubMed:28355180). Positively regulates translation of selected mRNAs through its binding to post-transcriptional control element (PCE) in the 5'-untranslated region (UTR) (PubMed:16680162). Involved with LARP6 in the translation stimulation of type I collagen mRNAs for CO1A1 and CO1A2 through binding of a specific stem-loop structure in their 5'-UTRs (PubMed:22190748). Stimulates LIN28A-dependent mRNA translation probably by facilitating ribonucleoprotein remodeling during the process of translation (PubMed:21247876). Plays also a role as a small interfering (siRNA)-loading factor involved in the RNA-induced silencing complex (RISC) loading complex (RLC) assembly, and hence functions in the RISC-mediated gene silencing process (PubMed:17531811). Binds preferentially to short double-stranded RNA, such as those produced during rotavirus intestinal infection (PubMed:28636595). This interaction may mediate NLRP9 inflammasome activation and trigger inflammatory response, including IL18 release and pyroptosis (PubMed:28636595). Finally, mediates the attachment of heterogeneous nuclear ribonucleoproteins (hnRNPs) to actin filaments in the nucleus (PubMed:11687588). {ECO:0000250|UniProtKB:O70133, ECO:0000269|PubMed:10198287, ECO:0000269|PubMed:10924507, ECO:0000269|PubMed:11038348, ECO:0000269|PubMed:11149922, ECO:0000269|PubMed:11402034, ECO:0000269|PubMed:11416126, ECO:0000269|PubMed:11687588, ECO:0000269|PubMed:12711669, ECO:0000269|PubMed:15355351, ECO:0000269|PubMed:1537828, ECO:0000269|PubMed:16680162, ECO:0000269|PubMed:17531811, ECO:0000269|PubMed:19029303, ECO:0000269|PubMed:20669935, ECO:0000269|PubMed:21247876, ECO:0000269|PubMed:21561811, ECO:0000269|PubMed:22190748, ECO:0000269|PubMed:24049074, ECO:0000269|PubMed:24990949, ECO:0000269|PubMed:25062910, ECO:0000269|PubMed:28221134, ECO:0000269|PubMed:28355180, ECO:0000269|PubMed:28636595, ECO:0000269|PubMed:9111062, ECO:0000269|PubMed:9162007, ECO:0000269|PubMed:9323138, ECO:0000269|PubMed:9662397}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DEAD|
|OB NTP bind|
|HA2|
|dsrm|
|Helicase C|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|regulation of viral translation|
|positive regulation of polysome binding|
|3-5 DNA/RNA helicase activity|
|DNA-templated viral transcription|
|positive regulation of viral translation|
|triplex DNA binding|
|regulation of polysome binding|
|regulatory region RNA binding|
|single-stranded 3-5 DNA helicase activity|
|RISC complex binding|
|positive regulation of interferon-beta secretion|
|targeting of mRNA for destruction involved in RNA interference|
|regulation of interferon-beta secretion|
|positive regulation of interleukin-18 production|
|CRD-mediated mRNA stabilization|
|perichromatin fibrils|
|nuclear stress granule|
|polysome binding|
|CRD-mediated mRNA stability complex|
|positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity|
|importin-alpha family protein binding|
|regulation of DNA topoisomerase (ATP-hydrolyzing) activity|
|protein localization to cytoplasmic stress granule|
|nucleoside-triphosphatase activity|
|G-quadruplex DNA unwinding|
|nucleoside-triphosphate diphosphatase activity|
|regulation of interleukin-18 production|
|positive regulation of RNA export from nucleus|
|RNA secondary structure unwinding|
|regulation of isomerase activity|
|positive regulation of nucleobase-containing compound transport|
|small RNA loading onto RISC|
|RISC-loading complex|
|positive regulation of isomerase activity|
|regulation of interferon-alpha secretion|
|positive regulation of interferon-alpha secretion|
|sequence-specific mRNA binding|
|siRNA binding|
|pyroptosis|
|positive regulation of cytoplasmic translation|
|regulation of RNA export from nucleus|
|RISC complex|
|RNA polymerase binding|
|regulation of nucleobase-containing compound transport|
|alternative mRNA splicing, via spliceosome|
|cellular response to exogenous dsRNA|
|RNA interference|
|3-5 DNA helicase activity|
|3-5 RNA helicase activity|
|positive regulation of tumor necrosis factor secretion|
|cellular response to dsRNA|
|RNA stem-loop binding|
|positive regulation of interferon-alpha production|
|positive regulation of gene silencing by miRNA|
|positive regulation of posttranscriptional gene silencing|
|DNA replication origin binding|
|regulation of interferon-alpha production|
|regulation of tumor necrosis factor secretion|
|regulation of cytoplasmic translation|
|positive regulation of interferon-beta production|
|positive regulation of interleukin-6 secretion|
|RNA polymerase II complex binding|
|polysomal ribosome|
|positive regulation of DNA replication|
|regulation of defense response to virus by host|
|positive regulation of viral transcription|
|mRNA stabilization|
|polysome|
|response to exogenous dsRNA|
|single-stranded RNA binding|
|regulation of interferon-beta production|
|RNA stabilization|
|promoter-specific chromatin binding|
|positive regulation of fibroblast proliferation|
|negative regulation of mRNA catabolic process|
|response to dsRNA|
|positive regulation of ATPase activity|
|posttranscriptional gene silencing by RNA|
|positive regulation of response to cytokine stimulus|
|posttranscriptional gene silencing|
|negative regulation of RNA catabolic process|
|DNA helicase activity|
|RNA polymerase II transcription factor binding|
|positive regulation of nucleocytoplasmic transport|
|RNA helicase activity|
|regulation of viral transcription|
|positive regulation of DNA repair|
|cytoplasmic ribonucleoprotein granule|
|chromatin DNA binding|
|double-stranded RNA binding|
|DNA-templated transcription, termination|
|regulation of defense response to virus|
|positive regulation of type I interferon production|
|negative regulation of mRNA metabolic process|
|regulation of ATPase activity|
|regulation of fibroblast proliferation|
|transcription coregulator activity|
|positive regulation of tumor necrosis factor production|
|regulation of gene silencing by miRNA|
|gene silencing by RNA|
|positive regulation of tumor necrosis factor superfamily cytokine production|
|regulation of gene silencing by RNA|
|regulation of posttranscriptional gene silencing|
|positive regulation of interleukin-6 production|
|positive regulation of response to DNA damage stimulus|
|double-stranded DNA binding|
|regulation of nucleocytoplasmic transport|
|positive regulation of viral process|
|single-stranded DNA binding|
|regulation of DNA replication|
|DNA duplex unwinding|
|viral transcription|
|DNA geometric change|
|regulation of DNA repair|
|positive regulation of translation|
|regulation of type I interferon production|
|osteoblast differentiation|
|viral gene expression|
|regulation of gene silencing|
|regulation of mRNA processing|
|positive regulation of inflammatory response|
|positive regulation of cytokine secretion|
|regulation of interleukin-6 production|
|regulation of tumor necrosis factor production|
|positive regulation of cellular amide metabolic process|
|gene silencing|
|regulation of tumor necrosis factor superfamily cytokine production|
|positive regulation of NF-kappaB transcription factor activity|
|ribonucleoprotein complex|
|mRNA transport|
|mRNA binding|
|regulation of response to cytokine stimulus|
|positive regulation of binding|
|regulation of mRNA stability|
|regulation of RNA stability|
|nucleic acid transport|
|RNA transport|
|positive regulation of DNA metabolic process|
|establishment of RNA localization|
|regulation of mRNA catabolic process|
|regulation of cytokine secretion|
|regulation of viral process|
|positive regulation of intracellular transport|
|DNA replication|
|RNA localization|
|actin cytoskeleton|
|regulation of response to DNA damage stimulus|
|regulation of symbiosis, encompassing mutualism through parasitism|
|ribonucleoprotein complex assembly|
|ATPase activity|
|regulation of gene expression, epigenetic|
|nucleobase-containing compound transport|
|cellular response to tumor necrosis factor|
|ribonucleoprotein complex subunit organization|
|negative regulation of cellular catabolic process|
|ossification|
|positive regulation of DNA-binding transcription factor activity|
|response to tumor necrosis factor|
|positive regulation of protein secretion|
|rhythmic process|
|nuclear body|
|transcription coactivator activity|
|positive regulation of peptide secretion|
|RNA splicing, via transesterification reactions with bulged adenosine as nucleophile|
|mRNA splicing, via spliceosome|
|DNA conformation change|
|RNA splicing, via transesterification reactions|
|negative regulation of catabolic process|
|regulation of mRNA metabolic process|
|regulation of inflammatory response|
|positive regulation of innate immune response|
|regulation of intracellular transport|
|regulation of translation|
|regulation of DNA metabolic process|
|positive regulation of response to biotic stimulus|
|regulation of binding|
|RNA splicing|
|positive regulation of secretion by cell|
|regulation of cellular amide metabolic process|
|positive regulation of protein transport|
|regulation of DNA-binding transcription factor activity|
|positive regulation of secretion|
|positive regulation of cytokine production|
|regulation of innate immune response|
|ribonucleoprotein complex biogenesis|
|positive regulation of establishment of protein localization|
|regulation of protein secretion|
|regulation of immune effector process|
|positive regulation of defense response|
|mRNA processing|
|centrosome|
|regulation of peptide secretion|
|inflammatory response|
|RNA polymerase II proximal promoter sequence-specific DNA binding|
|positive regulation of multi-organism process|
|regulation of response to biotic stimulus|
|posttranscriptional regulation of gene expression|
|cellular response to organic cyclic compound|
|protein-containing complex|
|positive regulation of response to external stimulus|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|RNA biosynthetic process|
|cellular response to nitrogen compound|
|mRNA metabolic process|
|regulation of cytokine production|
|viral process|
|regulation of protein transport|
|protein localization to organelle|
|regulation of cellular response to stress|
|regulation of peptide transport|
|regulation of establishment of protein localization|
|regulation of secretion by cell|
|regulation of defense response|
|innate immune response|
|positive regulation of hydrolase activity|
|regulation of multi-organism process|
|symbiotic process|
|regulation of secretion|
|interspecies interaction between organisms|
|regulation of cellular catabolic process|
|cellular protein-containing complex assembly|
|nucleolus|
|positive regulation of immune response|
|RNA processing|
|regulation of cellular localization|
|positive regulation of cell population proliferation|
|response to organic cyclic compound|
|defense response to other organism|
|positive regulation of transport|
|regulation of catabolic process|
|cellular response to cytokine stimulus|
|regulation of protein localization|
|programmed cell death|
|chromosome organization|
|response to nitrogen compound|
|cell death|
|regulation of response to external stimulus|
|nucleobase-containing compound biosynthetic process|
|response to cytokine|
|regulation of immune response|
|positive regulation of immune system process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|positive regulation of transcription by RNA polymerase II|
|regulation of hydrolase activity|
|response to other organism|
|organic cyclic compound biosynthetic process|
|response to external biotic stimulus|
|response to biotic stimulus|
|negative regulation of RNA metabolic process|
|defense response|
|RNA binding|
|positive regulation of catalytic activity|
|negative regulation of nucleobase-containing compound metabolic process|
|DNA binding|
|regulation of response to stress|
|ATP binding|
|positive regulation of transcription, DNA-templated|
|protein-containing complex assembly|
|cellular protein localization|
|cellular macromolecule localization|
|positive regulation of cellular protein metabolic process|
|regulation of cell population proliferation|
|cellular nitrogen compound biosynthetic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|regulation of immune system process|
|RNA metabolic process|
|positive regulation of protein metabolic process|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of multicellular organismal process|
|macromolecule biosynthetic process|
|positive regulation of molecular function|
|nitrogen compound transport|
|protein-containing complex subunit organization|
|regulation of transport|
|immune response|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|membrane|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp97|BI-6727 0.0125μM R03 exp97]]|-2.13|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|-1.83|
|[[:results:exp290|LLY-283 2.6μM R06 exp290]]|-1.8|
|[[:results:exp357|Dorsomorphin 5μM R07 exp357]]|-1.76|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|-1.76|
|[[:results:exp68|Clomiphene 4.4μM R02 exp68]]|1.73|
|[[:results:exp32|Rifampicin 10μM R00 exp32]]|1.86|
|[[:results:exp147|Resveratrol 16μM R03 exp147]]|1.97|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:z:zfand5|ZFAND5]]|0.453|
|[[:human genes:c:casc5|CASC5]]|0.414|
|[[:human genes:h:hap1|HAP1]]|0.408|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 72/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|0/1|
|bile duct|6/28|
|blood|2/28|
|bone|5/26|
|breast|5/33|
|central nervous system|5/56|
|cervix|2/4|
|colorectal|4/17|
|esophagus|0/13|
|fibroblast|1/1|
|gastric|0/16|
|kidney|0/21|
|liver|2/20|
|lung|5/75|
|lymphocyte|0/16|
|ovary|2/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|1/15|
|prostate|1/1|
|skin|1/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|3/22|
|urinary tract|3/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 234
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 9.23 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='DHX9 Expression in NALM6 Cells: 9.23'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>