ECT2 [The Human Chemical-Genetic Interaction Map Project]

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======= ECT2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: ECT2
  * **<color #00a2e8>Official Name</color>**: epithelial cell transforming 2
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=1894|1894]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9H8V3|Q9H8V3]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=ECT2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20ECT2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/600586|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)- induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. {ECO:0000269|PubMed:10579713, ECO:0000269|PubMed:14645260, ECO:0000269|PubMed:15254234, ECO:0000269|PubMed:15545273, ECO:0000269|PubMed:15642749, ECO:0000269|PubMed:16103226, ECO:0000269|PubMed:16170345, ECO:0000269|PubMed:16236794, ECO:0000269|PubMed:16495035, ECO:0000269|PubMed:19129481, ECO:0000269|PubMed:19468300, ECO:0000269|PubMed:19617897, ECO:0000269|PubMed:21189248, ECO:0000269|PubMed:21373644}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PTCB-BRCT|
|RhoGEF|
|BRCT|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|regulation of cytokinesis, actomyosin contractile ring assembly|
|regulation of cytokinetic process|
|centralspindlin complex|
|regulation of attachment of spindle microtubules to kinetochore|
|bicellular tight junction assembly|
|positive regulation of protein import into nucleus|
|tight junction assembly|
|tight junction organization|
|positive regulation of cytokinesis|
|positive regulation of protein import|
|apical junction assembly|
|regulation of protein import into nucleus|
|cleavage furrow|
|regulation of protein import|
|positive regulation of nucleocytoplasmic transport|
|mitotic cytokinesis|
|cellular response to ionizing radiation|
|Rho guanyl-nucleotide exchange factor activity|
|cellular response to hydrogen peroxide|
|positive regulation of protein localization to nucleus|
|mitotic spindle|
|cellular response to calcium ion|
|positive regulation of cell division|
|regulation of cytokinesis|
|regulation of actomyosin structure organization|
|cytoskeleton-dependent cytokinesis|
|activation of GTPase activity|
|cytokinesis|
|regulation of chromosome segregation|
|cell-cell junction assembly|
|regulation of nucleocytoplasmic transport|
|regulation of protein localization to nucleus|
|cellular response to antibiotic|
|response to hydrogen peroxide|
|bicellular tight junction|
|cellular response to reactive oxygen species|
|cell-cell junction organization|
|regulation of Rho protein signal transduction|
|response to ionizing radiation|
|response to calcium ion|
|positive regulation of intracellular protein transport|
|midbody|
|regulation of cell division|
|cell junction assembly|
|cell-cell junction|
|cellular response to radiation|
|positive regulation of I-kappaB kinase/NF-kappaB signaling|
|cellular response to metal ion|
|response to reactive oxygen species|
|positive regulation of intracellular transport|
|cellular response to toxic substance|
|cell junction organization|
|cellular response to inorganic substance|
|regulation of intracellular protein transport|
|regulation of I-kappaB kinase/NF-kappaB signaling|
|cellular response to oxidative stress|
|regulation of Ras protein signal transduction|
|GTPase activator activity|
|positive regulation of cell cycle process|
|response to antibiotic|
|cellular response to environmental stimulus|
|cellular response to abiotic stimulus|
|positive regulation of cellular protein localization|
|activation of protein kinase activity|
|protein homooligomerization|
|regulation of actin cytoskeleton organization|
|regulation of small GTPase mediated signal transduction|
|regulation of intracellular transport|
|positive regulation of neuron differentiation|
|response to metal ion|
|positive regulation of cell cycle|
|response to oxidative stress|
|regulation of actin filament-based process|
|cellular response to drug|
|positive regulation of GTPase activity|
|positive regulation of protein transport|
|response to radiation|
|positive regulation of establishment of protein localization|
|positive regulation of neurogenesis|
|regulation of GTPase activity|
|cell division|
|response to toxic substance|
|protein complex oligomerization|
|response to inorganic substance|
|positive regulation of protein kinase activity|
|positive regulation of nervous system development|
|regulation of cellular protein localization|
|regulation of cytoskeleton organization|
|positive regulation of cell development|
|positive regulation of kinase activity|
|mitotic cell cycle process|
|positive regulation of apoptotic process|
|positive regulation of programmed cell death|
|regulation of neuron differentiation|
|positive regulation of transferase activity|
|mitotic cell cycle|
|positive regulation of cell death|
|regulation of protein transport|
|cell morphogenesis|
|regulation of peptide transport|
|regulation of establishment of protein localization|
|regulation of cell cycle process|
|positive regulation of hydrolase activity|
|regulation of protein kinase activity|
|regulation of neurogenesis|
|cellular component morphogenesis|
|regulation of kinase activity|
|protein homodimerization activity|
|regulation of cellular localization|
|regulation of nervous system development|
|regulation of cell development|
|regulation of cellular component biogenesis|
|positive regulation of cell differentiation|
|regulation of transferase activity|
|positive regulation of transport|
|cell cycle process|
|positive regulation of protein phosphorylation|
|response to drug|
|positive regulation of intracellular signal transduction|
|regulation of protein localization|
|cellular response to oxygen-containing compound|
|positive regulation of phosphorylation|
|positive regulation of phosphate metabolic process|
|positive regulation of phosphorus metabolic process|
|response to abiotic stimulus|
|regulation of cell cycle|
|positive regulation of protein modification process|
|regulation of hydrolase activity|
|regulation of organelle organization|
|G protein-coupled receptor signaling pathway|
|cell cycle|
|positive regulation of developmental process|
|positive regulation of catalytic activity|
|regulation of protein phosphorylation|
|protein transport|
|generation of neurons|
|peptide transport|
|regulation of apoptotic process|
|response to oxygen-containing compound|
|protein-containing complex assembly|
|regulation of programmed cell death|
|amide transport|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|establishment of protein localization|
|neurogenesis|
|positive regulation of signal transduction|
|regulation of cell death|
|intracellular signal transduction|
|cellular response to stress|
|positive regulation of protein metabolic process|
|positive regulation of multicellular organismal process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of cell differentiation|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|nitrogen compound transport|
|regulation of protein modification process|
|protein-containing complex subunit organization|
|regulation of transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp64|Nocodazole 0.2μM R02 exp64]]|-2.12|
|[[:results:exp33|Rotenone 2μM R00 exp33]]|-2.02|
|[[:results:exp521|Salinomycin 3.5μM R08 exp521]]|-1.95|
|[[:results:exp382|Palbociclib 1μM R07 exp382]]|-1.94|
|[[:results:exp487|Hinokiflavone 12μM R08 exp487]]|-1.83|
|[[:results:exp469|CFI-400945 25μM R08 exp469]]|-1.77|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|-1.76|
|[[:results:exp65|Mubritinib 0.2μM R02 exp65]]|-1.73|
|[[:results:exp141|Nifurtimox 1μM R03 exp141]]|1.89|
|[[:results:exp237|NN-Diethyl-meta-toluamide 100μM R05 exp237]]|1.9|
|[[:results:exp7|Bortezomib 0.05μM R00 exp7]]|2.9|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:a:aurkb|AURKB]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 680/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|0/1|
|bile duct|25/28|
|blood|26/28|
|bone|23/26|
|breast|30/33|
|central nervous system|51/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|12/13|
|fibroblast|1/1|
|gastric|15/16|
|kidney|15/21|
|liver|18/20|
|lung|72/75|
|lymphocyte|16/16|
|ovary|25/26|
|pancreas|23/24|
|peripheral nervous system|15/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|22/24|
|soft tissue|9/9|
|thyroid|2/2|
|upper aerodigestive|21/22|
|urinary tract|27/29|
|uterus|3/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 831
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.61 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='ECT2 Expression in NALM6 Cells: 6.61'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>