EME1 [The Human Chemical-Genetic Interaction Map Project]

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======= EME1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: EME1
  * **<color #00a2e8>Official Name</color>**: essential meiotic structure-specific endonuclease 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=146956|146956]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q96AY2|Q96AY2]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=EME1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20EME1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/610885|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'- flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. {ECO:0000269|PubMed:12686547, ECO:0000269|PubMed:12721304, ECO:0000269|PubMed:14617801, ECO:0000269|PubMed:17289582}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|ERCC4|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|response to intra-S DNA damage checkpoint signaling|
|Holliday junction resolvase complex|
|response to cell cycle checkpoint signaling|
|response to DNA damage checkpoint signaling|
|response to DNA integrity checkpoint signaling|
|crossover junction endodeoxyribonuclease activity|
|intra-S DNA damage checkpoint|
|resolution of meiotic recombination intermediates|
|nuclear heterochromatin|
|meiotic chromosome separation|
|replication fork processing|
|chromosome separation|
|DNA-dependent DNA replication maintenance of fidelity|
|reciprocal meiotic recombination|
|interstrand cross-link repair|
|homologous recombination|
|meiotic chromosome segregation|
|mitotic DNA damage checkpoint|
|mitotic DNA integrity checkpoint|
|meiosis I|
|meiosis I cell cycle process|
|DNA-dependent DNA replication|
|DNA damage checkpoint|
|DNA integrity checkpoint|
|meiotic nuclear division|
|mitotic cell cycle checkpoint|
|meiotic cell cycle process|
|double-strand break repair|
|cell cycle checkpoint|
|DNA replication|
|cellular response to biotic stimulus|
|nuclear chromosome segregation|
|DNA recombination|
|meiotic cell cycle|
|nuclear chromatin|
|chromosome segregation|
|nuclear division|
|nucleic acid phosphodiester bond hydrolysis|
|negative regulation of mitotic cell cycle|
|organelle fission|
|DNA repair|
|negative regulation of cell cycle|
|mitotic cell cycle process|
|regulation of mitotic cell cycle|
|mitotic cell cycle|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|nucleolus|
|cell cycle process|
|regulation of cell cycle|
|cellular response to endogenous stimulus|
|response to biotic stimulus|
|cell cycle|
|reproductive process|
|reproduction|
|DNA binding|
|response to endogenous stimulus|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp64|Nocodazole 0.2μM R02 exp64]]|-2.18|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|-1.9|
|[[:results:exp72|LB-100 4.1μM R02 exp72]]|2.2|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:l:lig1|LIG1]]|0.435|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 1/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|1/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 3388
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.46 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='EME1 Expression in NALM6 Cells: 5.46'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>