EMP2 [The Human Chemical-Genetic Interaction Map Project]

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======= EMP2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: EMP2
  * **<color #00a2e8>Official Name</color>**: epithelial membrane protein 2
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=2013|2013]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P54851|P54851]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=EMP2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20EMP2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/602334|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a tetraspan protein of the PMP22/EMP family. The encoded protein regulates cell membrane composition. It has been associated with various functions including endocytosis, cell signaling, cell proliferation, cell migration, cell adhesion, cell death, cholesterol homeostasis, urinary albumin excretion, and embryo implantation. It is known to negatively regulate caveolin-1, a scaffolding protein which is the main component of the caveolae plasma membrane invaginations found in most cell types. Through activation of PTK2 it positively regulates vascular endothelial growth factor A. It also modulates the function of specific integrin isomers in the plasma membrane. Up-regulation of this gene has been linked to cancer progression in multiple different tissues. Mutations in this gene have been associated with nephrotic syndrome type 10 (NPHS10). [provided by RefSeq, Mar 2015]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Functions as a key regulator of cell membrane composition by regulating proteins surface expression. Also, plays a role in regulation of processes including cell migration, cell proliferation, cell contraction and cell adhesion. Negatively regulates caveolae formation by reducing CAV1 expression and CAV1 amount by increasing lysosomal degradation (PubMed:24814193). Facilitates surface trafficking and formation of lipid rafts bearing GPI-anchor proteins (By similarity). Regulates surface expression of MHC1 and ICAM1 proteins increasing susceptibility to T-cell mediated cytotoxicity (By similarity). Regulates the plasma membrane expression of the integrin heterodimers ITGA6-ITGB1, ITGA5-ITGB3 and ITGA5-ITGB1 resulting in modulation of cell-matrix adhesion (PubMed:16216233). Also regulates many processes through PTK2. Regulates blood vessel endothelial cell migration and angiogenesis by regulating VEGF protein expression through PTK2 activation (PubMed:23439602). Regulates cell migration and cell contraction through PTK2 and SRC activation (PubMed:21637765, PubMed:22728127). Regulates focal adhesion density, F-actin conformation and cell adhesion capacity through interaction with PTK2 (PubMed:19494199). Positively regulates cell proliferation (PubMed:24814193). Plays a role during cell death and cell blebbing (PubMed:12107182). Promotes angiogenesis and vasculogenesis through induction of VEGFA via a HIF1A-dependent pathway (PubMed:23334331). Also plays a role in embryo implantation by regulating surface trafficking of integrin heterodimer ITGA5-ITGB3 (PubMed:16487956). May play a role in glomerular filtration (By similarity). {ECO:0000250|UniProtKB:F1QIK8, ECO:0000250|UniProtKB:O88662, ECO:0000269|PubMed:12107182, ECO:0000269|PubMed:16216233, ECO:0000269|PubMed:16487956, ECO:0000269|PubMed:19494199, ECO:0000269|PubMed:21637765, ECO:0000269|PubMed:22728127, ECO:0000269|PubMed:23334331, ECO:0000269|PubMed:23439602, ECO:0000269|PubMed:24814193}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PMP22 Claudin|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|caveola assembly|
|plasma membrane raft assembly|
|positive regulation of integrin-mediated signaling pathway|
|plasma membrane raft organization|
|bleb assembly|
|regulation of glomerular filtration|
|membrane raft assembly|
|regulation of integrin-mediated signaling pathway|
|regulation of vasculogenesis|
|renal system process involved in regulation of blood volume|
|T cell mediated cytotoxicity|
|membrane raft organization|
|early endosome to late endosome transport|
|renal system process involved in regulation of systemic arterial blood pressure|
|vesicle-mediated transport between endosomal compartments|
|protein localization to cell surface|
|blood vessel endothelial cell migration|
|membrane assembly|
|regulation of renal system process|
|embryo implantation|
|membrane biogenesis|
|leukocyte mediated cytotoxicity|
|T cell mediated immunity|
|positive regulation of cell-matrix adhesion|
|endothelial cell migration|
|apical part of cell|
|caveola|
|epithelial cell migration|
|epithelium migration|
|cell killing|
|actin-mediated cell contraction|
|kinase binding|
|plasma membrane organization|
|regulation of systemic arterial blood pressure|
|tissue migration|
|actin filament-based movement|
|renal system process|
|regulation of cell-matrix adhesion|
|positive regulation of cell-substrate adhesion|
|cell-matrix adhesion|
|integrin binding|
|cytosolic transport|
|regulation of endothelial cell migration|
|ameboidal-type cell migration|
|female pregnancy|
|regulation of blood pressure|
|protein localization to plasma membrane|
|cell-substrate adhesion|
|regulation of cell-substrate adhesion|
|multi-multicellular organism process|
|regulation of epithelial cell migration|
|membrane raft|
|protein localization to cell periphery|
|actin filament organization|
|cytoplasmic vesicle|
|lymphocyte mediated immunity|
|adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains|
|regulation of angiogenesis|
|apical plasma membrane|
|regulation of vasculature development|
|activation of protein kinase activity|
|blood circulation|
|circulatory system process|
|positive regulation of cell adhesion|
|endomembrane system organization|
|plasma membrane bounded cell projection assembly|
|supramolecular fiber organization|
|cell projection assembly|
|protein kinase binding|
|protein localization to membrane|
|actin cytoskeleton organization|
|regulation of body fluid levels|
|positive regulation of protein kinase activity|
|cell population proliferation|
|actin filament-based process|
|positive regulation of kinase activity|
|regulation of system process|
|adaptive immune response|
|cell surface|
|Golgi membrane|
|positive regulation of transferase activity|
|regulation of cell adhesion|
|leukocyte mediated immunity|
|regulation of protein kinase activity|
|regulation of cell migration|
|membrane organization|
|regulation of kinase activity|
|regulation of cell motility|
|positive regulation of cell population proliferation|
|cell adhesion|
|biological adhesion|
|cell migration|
|regulation of transferase activity|
|regulation of locomotion|
|Golgi apparatus|
|regulation of cellular component movement|
|multi-organism reproductive process|
|positive regulation of protein phosphorylation|
|positive regulation of phosphorylation|
|regulation of anatomical structure morphogenesis|
|cell motility|
|localization of cell|
|immune effector process|
|cell death|
|cytoskeleton organization|
|plasma membrane bounded cell projection organization|
|positive regulation of phosphate metabolic process|
|positive regulation of phosphorus metabolic process|
|cell projection organization|
|positive regulation of protein modification process|
|locomotion|
|reproductive process|
|reproduction|
|positive regulation of catalytic activity|
|regulation of protein phosphorylation|
|intracellular transport|
|movement of cell or subcellular component|
|cellular protein localization|
|regulation of phosphorylation|
|cellular macromolecule localization|
|positive regulation of cellular protein metabolic process|
|regulation of cell population proliferation|
|positive regulation of signal transduction|
|positive regulation of protein metabolic process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of cell differentiation|
|positive regulation of cell communication|
|positive regulation of signaling|
|establishment of localization in cell|
|regulation of protein modification process|
|immune response|
|vesicle-mediated transport|
|system process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp319|ABT-702 5μM plus Dimethyloxaloylglycine 11μM R07 exp319]]|-2.01|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|-1.94|
|[[:results:exp340|BN82002 4μM R07 exp340]]|-1.87|
|[[:results:exp310|2-Methoxyestradiol 0.55μM R07 exp310]]|-1.73|
|[[:results:exp121|Golgicide-A 2μM R03 exp121]]|1.71|
|[[:results:exp141|Nifurtimox 1μM R03 exp141]]|1.81|
|[[:results:exp99|NFN1 0.4μM R03 exp99]]|1.98|
|[[:results:exp134|MS023 2μM R03 exp134]]|2.17|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 15042
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 5.26 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='EMP2 Expression in NALM6 Cells: 5.26'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>