EPS8 [The Human Chemical-Genetic Interaction Map Project]

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======= EPS8 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: EPS8
  * **<color #00a2e8>Official Name</color>**: epidermal growth factor receptor pathway substrate 8
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=2059|2059]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q12929|Q12929]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=EPS8&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20EPS8|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/600206|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Signaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate different processes. Together with SOS1 and ABI1, forms a trimeric complex that participates in transduction of signals from Ras to Rac by activating the Rac- specific guanine nucleotide exchange factor (GEF) activity. Acts as a direct regulator of actin dynamics by binding actin filaments and has both barbed-end actin filament capping and actin bundling activities depending on the context. Displays barbed-end actin capping activity when associated with ABI1, thereby regulating actin-based motility process: capping activity is auto-inhibited and inhibition is relieved upon ABI1 interaction. Also shows actin bundling activity when associated with BAIAP2, enhancing BAIAP2- dependent membrane extensions and promoting filopodial protrusions. Involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration and cancer cell migration and invasion. Acts as a regulator of axonal filopodia formation in neurons: in the absence of neurotrophic factors, negatively regulates axonal filopodia formation via actin-capping activity. In contrast, it is phosphorylated in the presence of BDNF leading to inhibition of its actin-capping activity and stimulation of filopodia formation. Component of a complex with WHRN and MYO15A that localizes at stereocilia tips and is required for elongation of the stereocilia actin core. Indirectly involved in cell cycle progression; its degradation following ubiquitination being required during G2 phase to promote cell shape changes. {ECO:0000269|PubMed:15558031, ECO:0000269|PubMed:17115031}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PTB|
|SH3 2|
|PID|
|SH3 1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|actin polymerization-dependent cell motility|
|actin crosslink formation|
|behavioral response to ethanol|
|NMDA selective glutamate receptor complex|
|signaling adaptor activity|
|exit from mitosis|
|barbed-end actin filament capping|
|positive regulation of ruffle assembly|
|stereocilium tip|
|Rac guanyl-nucleotide exchange factor activity|
|dendritic cell migration|
|Rac protein signal transduction|
|regulation of ruffle assembly|
|stereocilium|
|actin filament capping|
|negative regulation of actin filament depolymerization|
|regulation of postsynaptic membrane neurotransmitter receptor levels|
|regulation of actin filament depolymerization|
|brush border|
|negative regulation of actin filament polymerization|
|Rac GTPase binding|
|negative regulation of protein depolymerization|
|actin filament bundle assembly|
|actin cytoskeleton reorganization|
|negative regulation of protein polymerization|
|actin filament bundle organization|
|negative regulation of protein complex disassembly|
|adult locomotory behavior|
|Rho protein signal transduction|
|regulation of protein depolymerization|
|ruffle membrane|
|cellular response to leukemia inhibitory factor|
|response to leukemia inhibitory factor|
|positive regulation of plasma membrane bounded cell projection assembly|
|regulation of protein complex disassembly|
|response to ethanol|
|growth cone|
|negative regulation of supramolecular fiber organization|
|negative regulation of protein complex assembly|
|cell cortex|
|negative regulation of cytoskeleton organization|
|vesicle|
|regulation of Rho protein signal transduction|
|adult behavior|
|mitotic nuclear division|
|regulation of cell shape|
|regulation of actin filament polymerization|
|regulation of actin polymerization or depolymerization|
|regulation of actin filament length|
|locomotory behavior|
|regulation of plasma membrane bounded cell projection assembly|
|regulation of cell projection assembly|
|regulation of protein polymerization|
|response to alcohol|
|actin filament organization|
|regulation of Ras protein signal transduction|
|Ras protein signal transduction|
|postsynaptic density|
|regulation of actin filament organization|
|actin binding|
|mitotic cell cycle phase transition|
|cell cycle phase transition|
|nuclear division|
|response to antibiotic|
|organelle fission|
|small GTPase mediated signal transduction|
|regulation of actin cytoskeleton organization|
|regulation of small GTPase mediated signal transduction|
|glutamatergic synapse|
|regulation of supramolecular fiber organization|
|negative regulation of organelle organization|
|regulation of cellular component size|
|leukocyte migration|
|positive regulation of cell projection organization|
|regulation of actin filament-based process|
|regulation of protein complex assembly|
|supramolecular fiber organization|
|regulation of cell morphogenesis|
|actin cytoskeleton organization|
|response to toxic substance|
|regulation of anatomical structure size|
|positive regulation of cellular component biogenesis|
|regulation of cytoskeleton organization|
|cell junction|
|actin filament-based process|
|behavior|
|mitotic cell cycle process|
|mitotic cell cycle|
|regulation of plasma membrane bounded cell projection organization|
|negative regulation of cellular component organization|
|regulation of cell projection organization|
|regulation of cellular component biogenesis|
|cell migration|
|cell cycle process|
|cellular response to cytokine stimulus|
|response to drug|
|regulation of anatomical structure morphogenesis|
|cell motility|
|localization of cell|
|response to cytokine|
|cytoskeleton organization|
|positive regulation of cellular component organization|
|regulation of organelle organization|
|locomotion|
|cell cycle|
|movement of cell or subcellular component|
|response to oxygen-containing compound|
|positive regulation of signal transduction|
|intracellular signal transduction|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp37|Wortmannin 0.5μM R00 exp37]]|1.8|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 5587
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 0.47 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='EPS8 Expression in NALM6 Cells: 0.47'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>