ERN1 [The Human Chemical-Genetic Interaction Map Project]

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======= ERN1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: ERN1
  * **<color #00a2e8>Official Name</color>**: endoplasmic reticulum to nucleus signaling 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=2081|2081]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O75460|O75460]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=ERN1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20ERN1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/604033|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:24508390, PubMed:21317875). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:24508390, PubMed:21317875). {ECO:0000269|PubMed:11175748, ECO:0000269|PubMed:11779464, ECO:0000269|PubMed:12637535, ECO:0000269|PubMed:21317875, ECO:0000269|PubMed:9637683, ECO:0000305|PubMed:24508390}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Ribonuc 2-5A|
|Pkinase Tyr|
|Pkinase|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|Ire1 complex|
|mRNA splicing, via endonucleolytic cleavage and ligation|
|peptidyl-serine trans-autophosphorylation|
|protein trans-autophosphorylation|
|IRE1-RACK1-PP2A complex|
|AIP1-IRE1 complex|
|IRE1-TRAF2-ASK1 complex|
|insulin metabolic process|
|peptidyl-serine autophosphorylation|
|mRNA cleavage involved in mRNA processing|
|positive regulation of endoplasmic reticulum unfolded protein response|
|platelet-derived growth factor receptor binding|
|RNA splicing, via endonucleolytic cleavage and ligation|
|endothelial cell proliferation|
|mRNA cleavage|
|regulation of endoplasmic reticulum unfolded protein response|
|endoribonuclease activity|
|intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress|
|positive regulation of vascular smooth muscle cell proliferation|
|positive regulation of RNA splicing|
|positive regulation of response to endoplasmic reticulum stress|
|ADP binding|
|activation of JUN kinase activity|
|cellular response to vascular endothelial growth factor stimulus|
|Hsp90 protein binding|
|Hsp70 protein binding|
|nuclear inner membrane|
|regulation of vascular smooth muscle cell proliferation|
|IRE1-mediated unfolded protein response|
|cellular response to glucose stimulus|
|cellular response to hexose stimulus|
|positive regulation of JUN kinase activity|
|cellular response to monosaccharide stimulus|
|cellular response to carbohydrate stimulus|
|JNK cascade|
|RNA phosphodiester bond hydrolysis, endonucleolytic|
|regulation of response to endoplasmic reticulum stress|
|positive regulation of smooth muscle cell proliferation|
|epithelial cell proliferation|
|regulation of JUN kinase activity|
|cellular glucose homeostasis|
|endoplasmic reticulum unfolded protein response|
|stress-activated MAPK cascade|
|integral component of endoplasmic reticulum membrane|
|cellular response to unfolded protein|
|unfolded protein binding|
|regulation of smooth muscle cell proliferation|
|regulation of RNA splicing|
|response to glucose|
|positive regulation of JNK cascade|
|stress-activated protein kinase signaling cascade|
|response to hexose|
|cell cycle arrest|
|response to monosaccharide|
|intrinsic apoptotic signaling pathway|
|cellular response to topologically incorrect protein|
|activation of MAPK activity|
|RNA phosphodiester bond hydrolysis|
|response to unfolded protein|
|positive regulation of stress-activated MAPK cascade|
|positive regulation of stress-activated protein kinase signaling cascade|
|response to carbohydrate|
|regulation of macroautophagy|
|peptidyl-serine phosphorylation|
|glucose homeostasis|
|regulation of JNK cascade|
|carbohydrate homeostasis|
|response to topologically incorrect protein|
|protein autophosphorylation|
|peptidyl-serine modification|
|magnesium ion binding|
|mRNA catabolic process|
|regulation of stress-activated MAPK cascade|
|regulation of stress-activated protein kinase signaling cascade|
|protein kinase activity|
|RNA catabolic process|
|response to endoplasmic reticulum stress|
|positive regulation of MAP kinase activity|
|apoptotic signaling pathway|
|nucleic acid phosphodiester bond hydrolysis|
|activation of protein kinase activity|
|regulation of autophagy|
|positive regulation of protein serine/threonine kinase activity|
|regulation of MAP kinase activity|
|enzyme binding|
|protein serine/threonine kinase activity|
|MAPK cascade|
|nucleobase-containing compound catabolic process|
|signal transduction by protein phosphorylation|
|RNA splicing|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|aromatic compound catabolic process|
|mRNA processing|
|organic cyclic compound catabolic process|
|cellular response to growth factor stimulus|
|regulation of protein serine/threonine kinase activity|
|response to growth factor|
|positive regulation of protein kinase activity|
|positive regulation of MAPK cascade|
|cell population proliferation|
|negative regulation of cell cycle|
|positive regulation of kinase activity|
|positive regulation of transferase activity|
|mRNA metabolic process|
|regulation of cellular response to stress|
|cellular chemical homeostasis|
|regulation of MAPK cascade|
|regulation of protein kinase activity|
|regulation of cellular catabolic process|
|regulation of kinase activity|
|RNA processing|
|protein homodimerization activity|
|peptidyl-amino acid modification|
|cellular homeostasis|
|cellular macromolecule catabolic process|
|positive regulation of cell population proliferation|
|apoptotic process|
|endoplasmic reticulum membrane|
|protein phosphorylation|
|regulation of transferase activity|
|regulation of catabolic process|
|cell cycle process|
|endoplasmic reticulum|
|positive regulation of protein phosphorylation|
|positive regulation of intracellular signal transduction|
|macromolecule catabolic process|
|programmed cell death|
|cellular response to oxygen-containing compound|
|positive regulation of phosphorylation|
|identical protein binding|
|cell death|
|chemical homeostasis|
|positive regulation of phosphorus metabolic process|
|positive regulation of phosphate metabolic process|
|regulation of cell cycle|
|positive regulation of protein modification process|
|mitochondrion|
|phosphorylation|
|cell cycle|
|positive regulation of catalytic activity|
|regulation of protein phosphorylation|
|regulation of response to stress|
|ATP binding|
|response to oxygen-containing compound|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|regulation of cell population proliferation|
|homeostatic process|
|positive regulation of signal transduction|
|RNA metabolic process|
|intracellular signal transduction|
|cellular response to stress|
|positive regulation of protein metabolic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|organic substance catabolic process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|cellular catabolic process|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|regulation of protein modification process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of gene expression|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp8|Brefeldin A 0.02μM R00 exp8]]|-2.36|
|[[:results:exp451|Atovaquone 15μM R08 exp451]]|-2.2|
|[[:results:exp469|CFI-400945 25μM R08 exp469]]|-1.9|
|[[:results:exp525|Sulforaphane 9μM R08 exp525]]|-1.87|
|[[:results:exp139|Nicotinamide Riboside 100μM R03 exp139]]|-1.72|
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|1.7|
|[[:results:exp376|Losmapimod 1μM R07 exp376]]|1.95|
|[[:results:exp122|Golgicide-A 4μM R03 exp122]]|2|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 10653
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.15 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='ERN1 Expression in NALM6 Cells: 4.15'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>