GET4 [The Human Chemical-Genetic Interaction Map Project]

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======= GET4 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: GET4
  * **<color #00a2e8>Official Name</color>**: guided entry of tail-anchored proteins factor 4
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=51608|51608]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q7L5D6|Q7L5D6]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=GET4&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20GET4|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/612056|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20676083, PubMed:21636303, PubMed:21743475, PubMed:28104892). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum (PubMed:20676083, PubMed:28104892). Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome (PubMed:28104892). Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum (PubMed:21743475). The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (PubMed:21636303). {ECO:0000269|PubMed:20676083, ECO:0000269|PubMed:21636303, ECO:0000269|PubMed:21743475, ECO:0000269|PubMed:28104892}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DUF410|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|BAT3 complex|
|maintenance of unfolded protein involved in ERAD pathway|
|maintenance of unfolded protein|
|tail-anchored membrane protein insertion into ER membrane|
|protein insertion into ER membrane|
|cytoplasmic sequestering of protein|
|protein insertion into membrane|
|endoplasmic reticulum organization|
|ERAD pathway|
|chaperone binding|
|maintenance of protein location|
|maintenance of location|
|response to endoplasmic reticulum stress|
|establishment of protein localization to membrane|
|regulation of protein stability|
|proteasomal protein catabolic process|
|endomembrane system organization|
|protein localization to membrane|
|proteolysis involved in cellular protein catabolic process|
|cellular protein catabolic process|
|protein catabolic process|
|protein localization to organelle|
|membrane organization|
|nucleolus|
|cellular macromolecule catabolic process|
|response to organonitrogen compound|
|macromolecule catabolic process|
|organonitrogen compound catabolic process|
|response to nitrogen compound|
|proteolysis|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|cellular response to stress|
|organic substance catabolic process|
|cellular catabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp111|R-DABN 8μM R03 exp111]]|-2.59|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|-2.38|
|[[:results:exp475|CyclicAMP 200μM R08 exp475]]|-2.25|
|[[:results:exp128|GSK591 2.6μM R03 exp128]]|-2.1|
|[[:results:exp68|Clomiphene 4.4μM R02 exp68]]|-2.06|
|[[:results:exp164|Q15 1 to 2μM on day4 R04 exp164]]|-2.04|
|[[:results:exp222|Betulinic acid 10 to 15μM on day4 R05 exp222]]|-2|
|[[:results:exp454|Bafilomycin-A1 0.009μM R08 exp454]]|-1.99|
|[[:results:exp134|MS023 2μM R03 exp134]]|-1.99|
|[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|-1.98|
|[[:results:exp219|A-395N 10μM R05 exp219]]|-1.97|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|-1.96|
|[[:results:exp282|Fluvastatin 2.2μM R06 exp282]]|-1.96|
|[[:results:exp298|Sucrose 20000μM R06 exp298]]|-1.96|
|[[:results:exp280|Daidzin 10μM R06 exp280]]|-1.93|
|[[:results:exp442|Ibrutinib 10μM R08 exp442]]|-1.9|
|[[:results:exp483|FTY720 3μM R08 exp483]]|-1.88|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|-1.88|
|[[:results:exp432|YM155 0.001μM R08 exp432]]|-1.88|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|-1.79|
|[[:results:exp131|L-74142 5μM R03 exp131]]|-1.79|
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|-1.75|
|[[:results:exp122|Golgicide-A 4μM R03 exp122]]|-1.74|
|[[:results:exp12|Chloramphenicol 2μM R00 exp12]]|-1.73|
|[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|1.76|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:w:wrb|WRB]]|0.621|
|[[:human genes:p:ptar1|PTAR1]]|0.542|
|[[:human genes:s:scap|SCAP]]|0.447|
|[[:human genes:m:mbtps2|MBTPS2]]|0.439|
|[[:human genes:a:asna1|ASNA1]]|0.429|
|[[:human genes:c:cog7|COG7]]|0.423|
|[[:human genes:c:cog2|COG2]]|0.422|
|[[:human genes:h:htt|HTT]]|0.421|
|[[:human genes:p:pmvk|PMVK]]|0.411|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 16/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|1/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|6/21|
|liver|1/20|
|lung|3/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|1/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|1/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1479
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.89 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='GET4 Expression in NALM6 Cells: 4.89'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>