MCM4 [The Human Chemical-Genetic Interaction Map Project]

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======= MCM4 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: MCM4
  * **<color #00a2e8>Official Name</color>**: minichromosome maintenance complex component 4
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=4173|4173]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P33991|P33991]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=MCM4&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20MCM4|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/602638|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. {ECO:0000269|PubMed:16899510, ECO:0000269|PubMed:9305914}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|AAA 5|
|MCM|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|nuclear cell cycle DNA replication initiation|
|cell cycle DNA replication initiation|
|mitotic DNA replication initiation|
|pre-replicative complex assembly involved in nuclear cell cycle DNA replication|
|pre-replicative complex assembly involved in cell cycle DNA replication|
|pre-replicative complex assembly|
|mitotic DNA replication|
|double-strand break repair via break-induced replication|
|MCM complex|
|DNA unwinding involved in DNA replication|
|DNA strand elongation involved in DNA replication|
|DNA strand elongation|
|DNA replication origin binding|
|DNA replication initiation|
|nuclear DNA replication|
|cell cycle DNA replication|
|DNA helicase activity|
|double-strand break repair via homologous recombination|
|recombinational repair|
|single-stranded DNA binding|
|nuclear chromosome, telomeric region|
|DNA duplex unwinding|
|DNA geometric change|
|G1/S transition of mitotic cell cycle|
|DNA-dependent DNA replication|
|cell cycle G1/S phase transition|
|double-strand break repair|
|protein-DNA complex assembly|
|DNA replication|
|DNA recombination|
|protein-DNA complex subunit organization|
|mitotic cell cycle phase transition|
|cell cycle phase transition|
|DNA conformation change|
|DNA repair|
|mitotic cell cycle process|
|mitotic cell cycle|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|cellular protein-containing complex assembly|
|cell cycle process|
|chromosome organization|
|cell cycle|
|ATP binding|
|protein-containing complex assembly|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|protein-containing complex subunit organization|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp444|THZ531 0.225μM R08 exp444]]|-1.8|
|[[:results:exp467|CAY10603 0.55μM R08 exp467]]|-1.78|
|[[:results:exp82|Torin1 0.08μM R02 exp82]]|-1.74|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hgc6.3|HGC6.3]]|0.615|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 639/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|23/28|
|blood|24/28|
|bone|22/26|
|breast|27/33|
|central nervous system|53/56|
|cervix|3/4|
|colorectal|16/17|
|esophagus|10/13|
|fibroblast|1/1|
|gastric|15/16|
|kidney|18/21|
|liver|16/20|
|lung|65/75|
|lymphocyte|15/16|
|ovary|21/26|
|pancreas|23/24|
|peripheral nervous system|15/16|
|plasma cell|11/15|
|prostate|1/1|
|skin|21/24|
|soft tissue|8/9|
|thyroid|2/2|
|upper aerodigestive|10/22|
|urinary tract|24/29|
|uterus|4/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1127
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.77 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='MCM4 Expression in NALM6 Cells: 8.77'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>