MED12 [The Human Chemical-Genetic Interaction Map Project]

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======= MED12 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: MED12
  * **<color #00a2e8>Official Name</color>**: mediator complex subunit 12
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=9968|9968]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q93074|Q93074]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=MED12&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20MED12|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/300188|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. {ECO:0000269|PubMed:16565090, ECO:0000269|PubMed:16595664, ECO:0000269|PubMed:17000779}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Med12-LCEWAV|
|Med12-PQL|
|Med12|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|axis elongation involved in somitogenesis|
|embryonic neurocranium morphogenesis|
|embryonic brain development|
|vitamin D receptor binding|
|post-anal tail morphogenesis|
|axis elongation|
|Schwann cell development|
|thyroid hormone receptor binding|
|Schwann cell differentiation|
|mediator complex|
|oligodendrocyte development|
|embryonic cranial skeleton morphogenesis|
|somitogenesis|
|nuclear receptor transcription coactivator activity|
|cranial skeletal system development|
|oligodendrocyte differentiation|
|peripheral nervous system development|
|endoderm development|
|somite development|
|transcription coregulator activity|
|beta-catenin binding|
|canonical Wnt signaling pathway|
|neural tube closure|
|tube closure|
|embryonic skeletal system morphogenesis|
|segmentation|
|Wnt signaling pathway, planar cell polarity pathway|
|primary neural tube formation|
|RNA polymerase II distal enhancer sequence-specific DNA binding|
|ubiquitin ligase complex|
|neural tube formation|
|spinal cord development|
|glial cell development|
|regulation of establishment of planar polarity|
|developmental growth involved in morphogenesis|
|embryonic epithelial tube formation|
|embryonic skeletal system development|
|epithelial tube formation|
|non-canonical Wnt signaling pathway|
|stem cell population maintenance|
|maintenance of cell number|
|morphogenesis of embryonic epithelium|
|tube formation|
|neural tube development|
|glial cell differentiation|
|transcription initiation from RNA polymerase II promoter|
|regulation of morphogenesis of an epithelium|
|protein C-terminus binding|
|gliogenesis|
|DNA-templated transcription, initiation|
|anterior/posterior pattern specification|
|ubiquitin protein ligase activity|
|skeletal system morphogenesis|
|protein domain specific binding|
|regulation of animal organ morphogenesis|
|transcription coactivator activity|
|embryonic organ morphogenesis|
|epithelial tube morphogenesis|
|regionalization|
|Wnt signaling pathway|
|cell-cell signaling by wnt|
|developmental growth|
|growth|
|chromatin binding|
|cell surface receptor signaling pathway involved in cell-cell signaling|
|morphogenesis of an epithelium|
|embryonic organ development|
|pattern specification process|
|transcription by RNA polymerase II|
|skeletal system development|
|heart development|
|tissue morphogenesis|
|embryonic morphogenesis|
|chordate embryonic development|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|embryo development ending in birth or egg hatching|
|RNA biosynthetic process|
|tube morphogenesis|
|protein ubiquitination|
|protein modification by small protein conjugation|
|tube development|
|circulatory system development|
|anatomical structure formation involved in morphogenesis|
|animal organ morphogenesis|
|embryo development|
|central nervous system development|
|protein modification by small protein conjugation or removal|
|regulation of anatomical structure morphogenesis|
|nucleobase-containing compound biosynthetic process|
|epithelium development|
|cell-cell signaling|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|positive regulation of transcription by RNA polymerase II|
|organic cyclic compound biosynthetic process|
|positive regulation of transcription, DNA-templated|
|neurogenesis|
|cellular nitrogen compound biosynthetic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|cell development|
|RNA metabolic process|
|cellular macromolecule biosynthetic process|
|positive regulation of RNA metabolic process|
|tissue development|
|macromolecule biosynthetic process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|membrane|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|-2.13|
|[[:results:exp263|Aphidicolin 0.04μM R06 exp263]]|-2.11|
|[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|-2.08|
|[[:results:exp111|R-DABN 8μM R03 exp111]]|-2.07|
|[[:results:exp382|Palbociclib 1μM R07 exp382]]|-1.89|
|[[:results:exp96|BI-2536 0.02μM R03 exp96]]|-1.88|
|[[:results:exp431|Rotenone 0.07μM R08 exp431]]|-1.88|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|-1.83|
|[[:results:exp445|∆-9-Tetrahydrocannabinol 30μM R08 exp445]]|-1.77|
|[[:results:exp487|Hinokiflavone 12μM R08 exp487]]|-1.74|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|-1.7|
|[[:results:exp209|Deguelin 0.15μM R05 exp209]]|1.77|
|[[:results:exp237|NN-Diethyl-meta-toluamide 100μM R05 exp237]]|1.78|
|[[:results:exp210|LB-100 2μM R05 exp210]]|1.8|
|[[:results:exp152|SGC2043 10μM R03 exp152]]|1.85|
|[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|2.02|
|[[:results:exp159|Docetaxel 0.001 to 0.002μM on day4 R04 exp159]]|2.06|
|[[:results:exp215|Colchicine 0.009μM R05 exp215]]|2.11|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:a:aqr|AQR]]|0.496|
|[[:human genes:r:rrm1|RRM1]]|0.489|
|[[:human genes:t:tbc1d3g|TBC1D3G]]|0.466|
|[[:human genes:t:tbc1d3h|TBC1D3H]]|0.46|
|[[:human genes:a:aprt|APRT]]|0.455|
|[[:human genes:c:c14orf93|C14orf93]]|0.455|
|[[:human genes:p:psmd11|PSMD11]]|0.451|
|[[:human genes:v:vasn|VASN]]|0.447|
|[[:human genes:d:dtl|DTL]]|0.443|
|[[:human genes:t:tbc1d3f|TBC1D3F]]|0.442|
|[[:human genes:a:atxn1|ATXN1]]|0.439|
|[[:human genes:c:chmp2a|CHMP2A]]|0.439|
|[[:human genes:p:psap|PSAP]]|0.433|
|[[:human genes:m:med29|MED29]]|0.428|
|[[:human genes:z:znf106|ZNF106]]|0.426|
|[[:human genes:f:fbxo5|FBXO5]]|0.425|
|[[:human genes:r:rps12|RPS12]]|0.42|
|[[:human genes:u:ubap1|UBAP1]]|0.42|
|[[:human genes:p:pan2|PAN2]]|0.42|
|[[:human genes:p:polr2j3|POLR2J3]]|0.419|
|[[:human genes:s:slc38a7|SLC38A7]]|0.418|
|[[:human genes:s:sec13|SEC13]]|0.414|
|[[:human genes:c:chd4|CHD4]]|0.413|
|[[:human genes:m:mt1e|MT1E]]|0.413|
|[[:human genes:s:s1pr4|S1PR4]]|0.406|
|[[:human genes:z:zfand5|ZFAND5]]|0.406|
|[[:human genes:k:kif3c|KIF3C]]|0.404|
|[[:human genes:t:traip|TRAIP]]|0.403|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 161/683
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|0/1|
|bile duct|7/28|
|blood|12/26|
|bone|2/25|
|breast|10/30|
|central nervous system|9/49|
|cervix|3/4|
|colorectal|9/17|
|esophagus|3/11|
|fibroblast|0/1|
|gastric|4/14|
|kidney|2/18|
|liver|3/19|
|lung|8/72|
|lymphocyte|4/14|
|ovary|2/25|
|pancreas|5/22|
|peripheral nervous system|9/15|
|plasma cell|5/12|
|prostate|1/1|
|skin|7/20|
|soft tissue|1/7|
|thyroid|0/2|
|upper aerodigestive|9/22|
|urinary tract|7/28|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 780
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.34 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='MED12 Expression in NALM6 Cells: 6.34'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>