METTL16 [The Human Chemical-Genetic Interaction Map Project]

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======= METTL16 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: METTL16
  * **<color #00a2e8>Official Name</color>**: methyltransferase like 16
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=79066|79066]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q86W50|Q86W50]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=METTL16&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20METTL16|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**:  N/A

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  RNA N6-methyltransferase that methylates adenosine residues of a subset of RNAs and plays a key role in S-adenosyl-L- methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753). In presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression (PubMed:28525753). In S- adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6- methylation of U6 small nuclear RNA (U6 snRNA): specifically N6- methylates adenine in position 43 of U6 snRNAs (PubMed:28525753, PubMed:29051200). Also able to bind various lncRNAs (PubMed:29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311). {ECO:0000269|PubMed:27872311, ECO:0000269|PubMed:28525753, ECO:0000269|PubMed:29051200}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Methyltransf 10|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|U6 snRNA (adenine-(43)-N(6))-methyltransferase activity|
|U6 snRNA 3-end binding|
|snRNA (adenine-N6)-methylation|
|23S rRNA (adenine(1618)-N(6))-methyltransferase activity|
|mRNA (N6-adenosine)-methyltransferase activity|
|S-adenosylmethionine biosynthetic process|
|snRNA modification|
|rRNA base methylation|
|mRNA methylation|
|S-adenosylmethionine metabolic process|
|RNA stem-loop binding|
|mRNA modification|
|rRNA methylation|
|mRNA destabilization|
|RNA destabilization|
|rRNA modification|
|snRNA metabolic process|
|positive regulation of mRNA catabolic process|
|positive regulation of mRNA metabolic process|
|RNA methylation|
|regulation of mRNA splicing, via spliceosome|
|negative regulation of translation|
|regulation of RNA splicing|
|coenzyme biosynthetic process|
|regulation of mRNA processing|
|negative regulation of cellular amide metabolic process|
|RNA modification|
|regulation of mRNA stability|
|sulfur compound biosynthetic process|
|regulation of RNA stability|
|regulation of mRNA catabolic process|
|cofactor biosynthetic process|
|rRNA processing|
|mRNA catabolic process|
|rRNA metabolic process|
|RNA catabolic process|
|coenzyme metabolic process|
|macromolecule methylation|
|ribosome biogenesis|
|methylation|
|regulation of mRNA metabolic process|
|regulation of translation|
|positive regulation of cellular catabolic process|
|sulfur compound metabolic process|
|nucleobase-containing compound catabolic process|
|ncRNA processing|
|regulation of cellular amide metabolic process|
|positive regulation of catabolic process|
|heterocycle catabolic process|
|cellular nitrogen compound catabolic process|
|cofactor metabolic process|
|aromatic compound catabolic process|
|ribonucleoprotein complex biogenesis|
|ncRNA metabolic process|
|organic cyclic compound catabolic process|
|drug metabolic process|
|posttranscriptional regulation of gene expression|
|mRNA metabolic process|
|regulation of cellular catabolic process|
|RNA processing|
|cellular macromolecule catabolic process|
|regulation of catabolic process|
|negative regulation of cellular protein metabolic process|
|macromolecule catabolic process|
|negative regulation of protein metabolic process|
|negative regulation of cellular macromolecule biosynthetic process|
|RNA binding|
|negative regulation of macromolecule biosynthetic process|
|negative regulation of cellular biosynthetic process|
|negative regulation of biosynthetic process|
|RNA metabolic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|organic substance catabolic process|
|cellular catabolic process|
|positive regulation of nucleobase-containing compound metabolic process|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp77|Prochlorperazine 5.2μM R02 exp77]]|-2.23|
|[[:results:exp248|UM0131023 0.05μM R05 exp248]]|-2.21|
|[[:results:exp37|Wortmannin 0.5μM R00 exp37]]|-2.18|
|[[:results:exp41|BI-2536 0.001μM R01 exp41]]|-2.03|
|[[:results:exp12|Chloramphenicol 2μM R00 exp12]]|-1.9|
|[[:results:exp59|UMK57 1μM R01 exp59]]|-1.85|
|[[:results:exp54|Taxol 0.002μM R01 exp54]]|-1.82|
|[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|-1.81|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|-1.73|
|[[:results:exp155|UNC1999 2μM R03 exp155]]|1.76|
|[[:results:exp349|Cytochalasin-B 5μM R07 exp349]]|1.77|
|[[:results:exp80|RO-3307 4.7μM R02 exp80]]|1.8|
|[[:results:exp288|HMS-I2 10μM R06 exp288]]|1.84|
|[[:results:exp120|Dimethyl-Sulfoxide 1pc R03 exp120]]|1.96|
|[[:results:exp518|RK-33 8μM R08 exp518]]|2.02|
|[[:results:exp135|MS023 7μM R03 exp135]]|3.02|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 681/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|1/1|
|bile duct|27/28|
|blood|28/28|
|bone|25/26|
|breast|30/33|
|central nervous system|53/56|
|cervix|4/4|
|colorectal|16/17|
|esophagus|12/13|
|fibroblast|1/1|
|gastric|14/16|
|kidney|18/21|
|liver|17/20|
|lung|69/75|
|lymphocyte|14/16|
|ovary|24/26|
|pancreas|24/24|
|peripheral nervous system|14/16|
|plasma cell|10/15|
|prostate|0/1|
|skin|17/24|
|soft tissue|8/9|
|thyroid|2/2|
|upper aerodigestive|21/22|
|urinary tract|27/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 729
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.67 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='METTL16 Expression in NALM6 Cells: 6.67'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>