NGLY1 [The Human Chemical-Genetic Interaction Map Project]

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======= NGLY1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: NGLY1
  * **<color #00a2e8>Official Name</color>**: N-glycanase 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=55768|55768]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q96IV0|Q96IV0]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=NGLY1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20NGLY1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/610661|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Specifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. {ECO:0000269|PubMed:14749736, ECO:0000269|PubMed:15358861}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Rad4|
|Transglut core|
|PUB|
|DUF750|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity|
|glycoprotein catabolic process|
|protein quality control for misfolded or incompletely synthesized proteins|
|protein deglycosylation|
|carbohydrate derivative catabolic process|
|protein folding|
|glycoprotein metabolic process|
|proteolysis involved in cellular protein catabolic process|
|cellular protein catabolic process|
|protein catabolic process|
|cellular macromolecule catabolic process|
|carbohydrate derivative metabolic process|
|macromolecule catabolic process|
|organonitrogen compound catabolic process|
|proteolysis|
|organic substance catabolic process|
|cellular catabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp6|Bortezomib 0.005μM R00 exp6]]|-3.49|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|-3.35|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|-3.33|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|-3.3|
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|-2.97|
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|-2.8|
|[[:results:exp234|Ethanol 0.01 R05 exp234]]|-2.78|
|[[:results:exp277|Curcumin 6.5μM R06 exp277]]|-2.72|
|[[:results:exp264|Arsenate 40μM R06 exp264]]|-2.38|
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|-2.34|
|[[:results:exp449|Arsenic trioxide 60μM R08 exp449]]|-2.31|
|[[:results:exp102|Nifuroxazide 5μM R03 exp102]]|-2.24|
|[[:results:exp180|Dynasore 10μM R04 exp180]]|-2.22|
|[[:results:exp182|IU1-47 25μM R04 exp182]]|-1.9|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|-1.84|
|[[:results:exp227|Cryptotanshinone 12μM R05 exp227]]|-1.79|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|-1.7|
|[[:results:exp38|Wortmannin 5μM R00 exp38]]|1.71|
|[[:results:exp113|1-Methyl-nicotinamide-chloride 1000μM R03 exp113]]|1.76|
|[[:results:exp30|Rapamycin 10μM R00 exp30]]|1.77|
|[[:results:exp132|Metformin 40μM R03 exp132]]|1.8|
|[[:results:exp150|SGC0649 7μM R03 exp150]]|1.98|
|[[:results:exp14|Cycloheximide 0.02μM R00 exp14]]|2.18|
|[[:results:exp12|Chloramphenicol 2μM R00 exp12]]|2.57|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:d:ddi2|DDI2]]|0.683|
|[[:human genes:n:nfe2l1|NFE2L1]]|0.662|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 4/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|1/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|1/75|
|lymphocyte|0/16|
|ovary|1/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2297
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.42 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='NGLY1 Expression in NALM6 Cells: 6.42'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>