PCK1 [The Human Chemical-Genetic Interaction Map Project]

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======= PCK1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PCK1
  * **<color #00a2e8>Official Name</color>**: phosphoenolpyruvate carboxykinase 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5105|5105]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P35558|P35558]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PCK1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PCK1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/614168|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. {ECO:0000269|PubMed:24863970, ECO:0000269|PubMed:26971250, ECO:0000269|PubMed:28216384}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PEPCK|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|positive regulation of transcription from RNA polymerase II promoter in response to acidic pH|
|propionate catabolic process|
|glycerol biosynthetic process from pyruvate|
|phosphoenolpyruvate carboxykinase (GTP) activity|
|cellular response to potassium ion starvation|
|response to methionine|
|glycerol biosynthetic process|
|cellular response to fructose stimulus|
|propionate metabolic process|
|alditol biosynthetic process|
|carboxylic acid binding|
|oxaloacetate metabolic process|
|response to fructose|
|short-chain fatty acid catabolic process|
|cellular response to acidic pH|
|hepatocyte differentiation|
|short-chain fatty acid metabolic process|
|triglyceride biosynthetic process|
|glycerol metabolic process|
|acylglycerol biosynthetic process|
|neutral lipid biosynthetic process|
|response to acidic pH|
|cellular response to pH|
|alditol metabolic process|
|cellular response to glucagon stimulus|
|cellular response to dexamethasone stimulus|
|response to interleukin-6|
|response to glucagon|
|response to pH|
|response to dexamethasone|
|gluconeogenesis|
|hexose biosynthetic process|
|antibiotic catabolic process|
|monosaccharide biosynthetic process|
|cellular response to cAMP|
|cellular response to glucocorticoid stimulus|
|cellular response to corticosteroid stimulus|
|polyol biosynthetic process|
|cellular carbohydrate biosynthetic process|
|manganese ion binding|
|response to activity|
|cellular response to glucose stimulus|
|cellular response to retinoic acid|
|pyruvate metabolic process|
|cellular response to hexose stimulus|
|cellular response to monosaccharide stimulus|
|GDP binding|
|triglyceride metabolic process|
|cellular response to carbohydrate stimulus|
|cellular glucose homeostasis|
|cellular response to ketone|
|fatty acid catabolic process|
|response to antineoplastic agent|
|dicarboxylic acid metabolic process|
|acylglycerol metabolic process|
|response to cAMP|
|neutral lipid metabolic process|
|antibiotic metabolic process|
|response to retinoic acid|
|polyol metabolic process|
|alcohol biosynthetic process|
|glucose metabolic process|
|response to amino acid|
|monocarboxylic acid catabolic process|
|internal protein amino acid acetylation|
|liver development|
|carbohydrate biosynthetic process|
|hepaticobiliary system development|
|response to organophosphorus|
|response to glucose|
|response to hexose|
|protein acetylation|
|drug catabolic process|
|response to glucocorticoid|
|response to monosaccharide|
|cellular response to starvation|
|response to purine-containing compound|
|hexose metabolic process|
|response to corticosteroid|
|cellular response to insulin stimulus|
|cellular carbohydrate metabolic process|
|response to carbohydrate|
|cellular response to interleukin-1|
|glucose homeostasis|
|organic hydroxy compound biosynthetic process|
|carbohydrate homeostasis|
|cellular response to xenobiotic stimulus|
|protein acylation|
|cellular response to steroid hormone stimulus|
|cellular response to hypoxia|
|response to ketone|
|response to starvation|
|cellular lipid catabolic process|
|cellular response to decreased oxygen levels|
|response to interleukin-1|
|monosaccharide metabolic process|
|cellular response to acid chemical|
|cellular response to oxygen levels|
|magnesium ion binding|
|glycerolipid biosynthetic process|
|response to insulin|
|cellular response to nutrient levels|
|cellular response to tumor necrosis factor|
|response to tumor necrosis factor|
|cellular response to extracellular stimulus|
|carboxylic acid catabolic process|
|organic acid catabolic process|
|cellular response to peptide hormone stimulus|
|aging|
|lipid catabolic process|
|response to xenobiotic stimulus|
|cellular response to abiotic stimulus|
|cellular response to environmental stimulus|
|response to lipopolysaccharide|
|alcohol metabolic process|
|fatty acid metabolic process|
|cellular response to peptide|
|response to molecule of bacterial origin|
|response to steroid hormone|
|cellular response to external stimulus|
|response to hypoxia|
|response to acid chemical|
|response to decreased oxygen levels|
|glycerolipid metabolic process|
|response to oxygen levels|
|GTP binding|
|response to peptide hormone|
|cellular response to drug|
|gland development|
|small molecule catabolic process|
|organic hydroxy compound metabolic process|
|response to peptide|
|carbohydrate metabolic process|
|response to nutrient levels|
|drug metabolic process|
|cellular response to lipid|
|monocarboxylic acid metabolic process|
|response to extracellular stimulus|
|cellular response to organic cyclic compound|
|lipid biosynthetic process|
|small molecule biosynthetic process|
|cellular response to organonitrogen compound|
|cellular response to hormone stimulus|
|cellular response to nitrogen compound|
|epithelial cell differentiation|
|response to bacterium|
|cellular chemical homeostasis|
|response to lipid|
|cellular homeostasis|
|carboxylic acid metabolic process|
|response to hormone|
|response to organic cyclic compound|
|cellular lipid metabolic process|
|response to organonitrogen compound|
|oxoacid metabolic process|
|cellular response to cytokine stimulus|
|response to drug|
|organic acid metabolic process|
|cellular response to oxygen-containing compound|
|response to nitrogen compound|
|response to cytokine|
|chemical homeostasis|
|epithelium development|
|response to abiotic stimulus|
|lipid metabolic process|
|positive regulation of transcription by RNA polymerase II|
|cellular response to endogenous stimulus|
|mitochondrion|
|response to other organism|
|response to external biotic stimulus|
|response to biotic stimulus|
|response to endogenous stimulus|
|positive regulation of transcription, DNA-templated|
|response to oxygen-containing compound|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|homeostatic process|
|cellular response to stress|
|positive regulation of RNA metabolic process|
|small molecule metabolic process|
|tissue development|
|organic substance catabolic process|
|cellular catabolic process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp501|Methotrexate 0.01μM R08 exp501]]|-2.09|
|[[:results:exp13|Chloramphenicol 20μM R00 exp13]]|-1.96|
|[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|-1.85|
|[[:results:exp17|DABN 20μM R00 exp17]]|-1.7|
|[[:results:exp219|A-395N 10μM R05 exp219]]|1.71|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 5936
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: -0.28 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PCK1 Expression in NALM6 Cells: -0.28'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>