PGK1 [The Human Chemical-Genetic Interaction Map Project]

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======= PGK1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PGK1
  * **<color #00a2e8>Official Name</color>**: phosphoglycerate kinase 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5230|5230]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P00558|P00558]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PGK1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PGK1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/311800|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]. 
  * **<color #00a2e8>UniProt Summary</color>**:  In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959). {ECO:0000269|PubMed:2324090, ECO:0000269|PubMed:26677959}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PGK|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|phosphoglycerate kinase activity|
|protein-disulfide reductase activity|
|positive regulation of oxidative phosphorylation|
|plasminogen activation|
|NADH regeneration|
|glucose catabolic process to pyruvate|
|canonical glycolysis|
|glycolytic process through glucose-6-phosphate|
|glycolytic process through fructose-6-phosphate|
|regulation of oxidative phosphorylation|
|glucose catabolic process|
|NADH metabolic process|
|ADP binding|
|glycolytic process|
|zymogen activation|
|ATP generation from ADP|
|gluconeogenesis|
|positive regulation of ATP metabolic process|
|NAD metabolic process|
|hexose biosynthetic process|
|ADP metabolic process|
|hexose catabolic process|
|monosaccharide biosynthetic process|
|nucleoside diphosphate phosphorylation|
|purine ribonucleoside diphosphate metabolic process|
|nucleotide phosphorylation|
|purine nucleoside diphosphate metabolic process|
|monosaccharide catabolic process|
|ribonucleoside diphosphate metabolic process|
|pyruvate metabolic process|
|nucleoside diphosphate metabolic process|
|negative regulation of angiogenesis|
|negative regulation of blood vessel morphogenesis|
|glucose metabolic process|
|negative regulation of vasculature development|
|carbohydrate catabolic process|
|regulation of ATP metabolic process|
|carbohydrate biosynthetic process|
|hexose metabolic process|
|protein processing|
|regulation of generation of precursor metabolites and energy|
|cellular response to hypoxia|
|cellular response to decreased oxygen levels|
|monosaccharide metabolic process|
|ATP metabolic process|
|cellular response to oxygen levels|
|membrane raft|
|protein maturation|
|regulation of angiogenesis|
|purine ribonucleotide metabolic process|
|regulation of vasculature development|
|ribonucleotide metabolic process|
|purine nucleotide metabolic process|
|ribose phosphate metabolic process|
|response to hypoxia|
|response to decreased oxygen levels|
|purine-containing compound metabolic process|
|response to oxygen levels|
|generation of precursor metabolites and energy|
|small molecule catabolic process|
|nucleotide metabolic process|
|nucleoside phosphate metabolic process|
|carbohydrate metabolic process|
|monocarboxylic acid metabolic process|
|nucleobase-containing small molecule metabolic process|
|small molecule biosynthetic process|
|epithelial cell differentiation|
|organophosphate metabolic process|
|carboxylic acid metabolic process|
|negative regulation of developmental process|
|oxidation-reduction process|
|oxoacid metabolic process|
|organic acid metabolic process|
|carbohydrate derivative metabolic process|
|positive regulation of phosphorylation|
|regulation of anatomical structure morphogenesis|
|epithelium development|
|positive regulation of phosphorus metabolic process|
|positive regulation of phosphate metabolic process|
|response to abiotic stimulus|
|negative regulation of multicellular organismal process|
|proteolysis|
|phosphorylation|
|ATP binding|
|regulation of phosphorylation|
|extracellular space|
|cellular response to stress|
|small molecule metabolic process|
|tissue development|
|organic substance catabolic process|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|membrane|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp286|HMS-I2 1μM R06 exp286]]|-1.91|
|[[:results:exp490|Hydroxychloroquine 30μM R08 exp490]]|-1.9|
|[[:results:exp342|Calcium Ionophore 0.4μM R07 exp342]]|-1.88|
|[[:results:exp220|BAY-598 4μM R05 exp220]]|-1.72|
|[[:results:exp349|Cytochalasin-B 5μM R07 exp349]]|1.81|
|[[:results:exp469|CFI-400945 25μM R08 exp469]]|1.85|
|[[:results:exp70|INK128 0.2μM R02 exp70]]|2.02|
|[[:results:exp401|SNS-032 25μM R07 exp401]]|2.09|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|2.38|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:t:tpi1|TPI1]]|0.53|
|[[:human genes:e:eno1|ENO1]]|0.528|
|[[:human genes:p:pkm|PKM]]|0.51|
|[[:human genes:t:tkt|TKT]]|0.493|
|[[:human genes:r:rpe|RPE]]|0.462|
|[[:human genes:s:srp14|SRP14]]|0.452|
|[[:human genes:r:rabggtb|RABGGTB]]|0.452|
|[[:human genes:g:gemin8|GEMIN8]]|0.443|
|[[:human genes:r:rpia|RPIA]]|0.441|
|[[:human genes:a:aasdhppt|AASDHPPT]]|0.428|
|[[:human genes:d:ddx20|DDX20]]|0.414|
|[[:human genes:n:nsmce1|NSMCE1]]|0.41|
|[[:human genes:p:pmm2|PMM2]]|0.406|
|[[:human genes:t:tgs1|TGS1]]|0.402|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 607/694
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|23/28|
|blood|23/26|
|bone|26/26|
|breast|22/30|
|central nervous system|45/49|
|cervix|2/4|
|colorectal|14/17|
|esophagus|11/11|
|fibroblast|1/1|
|gastric|10/14|
|kidney|16/18|
|liver|15/19|
|lung|58/72|
|lymphocyte|14/16|
|ovary|20/25|
|pancreas|21/22|
|peripheral nervous system|13/15|
|plasma cell|10/12|
|prostate|0/1|
|skin|17/20|
|soft tissue|9/9|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|26/28|
|uterus|4/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 476
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.77 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PGK1 Expression in NALM6 Cells: 8.77'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>