PKM [The Human Chemical-Genetic Interaction Map Project]

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======= PKM =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PKM
  * **<color #00a2e8>Official Name</color>**: pyruvate kinase M1/2
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5315|5315]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P14618|P14618]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PKM&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PKM|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/179050|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]. 
  * **<color #00a2e8>UniProt Summary</color>**: N/A

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PK|
|PK C|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|pyruvate kinase complex|
|pyruvate kinase activity|
|pyruvate biosynthetic process|
|thyroid hormone binding|
|response to muscle inactivity|
|response to gravity|
|potassium ion binding|
|response to inactivity|
|MHC class II protein complex binding|
|glucose catabolic process to pyruvate|
|NADH regeneration|
|canonical glycolysis|
|glycolytic process through fructose-6-phosphate|
|glycolytic process through glucose-6-phosphate|
|glucose catabolic process|
|ATP biosynthetic process|
|skeletal muscle tissue regeneration|
|NADH metabolic process|
|ADP binding|
|positive regulation of sprouting angiogenesis|
|glycolytic process|
|ATP generation from ADP|
|purine ribonucleoside triphosphate biosynthetic process|
|NAD metabolic process|
|purine nucleoside triphosphate biosynthetic process|
|ADP metabolic process|
|ribonucleoside triphosphate biosynthetic process|
|hexose catabolic process|
|extracellular vesicle|
|purine ribonucleoside triphosphate metabolic process|
|nucleoside diphosphate phosphorylation|
|nucleotide phosphorylation|
|purine nucleoside diphosphate metabolic process|
|monosaccharide catabolic process|
|purine ribonucleoside diphosphate metabolic process|
|nucleoside triphosphate biosynthetic process|
|tissue regeneration|
|ribonucleoside diphosphate metabolic process|
|ribonucleoside triphosphate metabolic process|
|purine nucleoside triphosphate metabolic process|
|regulation of sprouting angiogenesis|
|pyruvate metabolic process|
|animal organ regeneration|
|kinase activity|
|nucleoside diphosphate metabolic process|
|nucleoside triphosphate metabolic process|
|protein homotetramerization|
|glucose metabolic process|
|secretory granule lumen|
|carbohydrate catabolic process|
|ficolin-1-rich granule lumen|
|liver development|
|hepaticobiliary system development|
|vesicle|
|purine ribonucleotide biosynthetic process|
|protein tetramerization|
|hexose metabolic process|
|regeneration|
|purine nucleotide biosynthetic process|
|ribonucleotide biosynthetic process|
|cellular response to insulin stimulus|
|positive regulation of angiogenesis|
|ribose phosphate biosynthetic process|
|purine-containing compound biosynthetic process|
|positive regulation of vasculature development|
|monocarboxylic acid biosynthetic process|
|cilium|
|monosaccharide metabolic process|
|ATP metabolic process|
|response to nutrient|
|magnesium ion binding|
|response to insulin|
|nucleotide biosynthetic process|
|nucleoside phosphate biosynthetic process|
|cellular response to peptide hormone stimulus|
|regulation of angiogenesis|
|carboxylic acid biosynthetic process|
|organic acid biosynthetic process|
|cadherin binding|
|purine ribonucleotide metabolic process|
|regulation of vasculature development|
|ribonucleotide metabolic process|
|cellular response to peptide|
|purine nucleotide metabolic process|
|ribose phosphate metabolic process|
|protein homooligomerization|
|response to hypoxia|
|response to decreased oxygen levels|
|collagen-containing extracellular matrix|
|purine-containing compound metabolic process|
|response to oxygen levels|
|developmental growth|
|response to peptide hormone|
|growth|
|gland development|
|generation of precursor metabolites and energy|
|small molecule catabolic process|
|nucleotide metabolic process|
|nucleoside phosphate metabolic process|
|response to peptide|
|carbohydrate metabolic process|
|wound healing|
|neutrophil degranulation|
|neutrophil activation involved in immune response|
|neutrophil mediated immunity|
|response to nutrient levels|
|drug metabolic process|
|neutrophil activation|
|granulocyte activation|
|leukocyte degranulation|
|myeloid leukocyte mediated immunity|
|protein complex oligomerization|
|myeloid cell activation involved in immune response|
|monocarboxylic acid metabolic process|
|response to extracellular stimulus|
|organophosphate biosynthetic process|
|nucleobase-containing small molecule metabolic process|
|response to wounding|
|myeloid leukocyte activation|
|small molecule biosynthetic process|
|cellular response to organonitrogen compound|
|cellular response to hormone stimulus|
|carbohydrate derivative biosynthetic process|
|leukocyte activation involved in immune response|
|cell activation involved in immune response|
|cellular response to nitrogen compound|
|regulated exocytosis|
|leukocyte mediated immunity|
|exocytosis|
|organophosphate metabolic process|
|response to hormone|
|carboxylic acid metabolic process|
|leukocyte activation|
|oxidation-reduction process|
|response to organonitrogen compound|
|oxoacid metabolic process|
|secretion by cell|
|organic acid metabolic process|
|carbohydrate derivative metabolic process|
|export from cell|
|programmed cell death|
|cellular response to oxygen-containing compound|
|regulation of anatomical structure morphogenesis|
|identical protein binding|
|cell activation|
|response to nitrogen compound|
|cell death|
|immune effector process|
|nucleobase-containing compound biosynthetic process|
|secretion|
|response to abiotic stimulus|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|cellular response to endogenous stimulus|
|mitochondrion|
|phosphorylation|
|organic cyclic compound biosynthetic process|
|positive regulation of developmental process|
|RNA binding|
|organonitrogen compound biosynthetic process|
|response to endogenous stimulus|
|ATP binding|
|response to oxygen-containing compound|
|protein-containing complex assembly|
|cellular nitrogen compound biosynthetic process|
|positive regulation of multicellular organismal process|
|small molecule metabolic process|
|tissue development|
|organic substance catabolic process|
|protein-containing complex subunit organization|
|immune response|
|extracellular region|
|vesicle-mediated transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp183|IU1-C 25μM R04 exp183]]|-2.16|
|[[:results:exp246|UM0011500 10μM R05 exp246]]|-1.94|
|[[:results:exp159|Docetaxel 0.001 to 0.002μM on day4 R04 exp159]]|-1.75|
|[[:results:exp342|Calcium Ionophore 0.4μM R07 exp342]]|-1.71|
|[[:results:exp374|Latrunculin-B 10μM R07 exp374]]|1.91|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:e:eno1|ENO1]]|0.528|
|[[:human genes:p:pgk1|PGK1]]|0.51|
|[[:human genes:t:tpi1|TPI1]]|0.478|
|[[:human genes:t:tkt|TKT]]|0.477|
|[[:human genes:r:rpe|RPE]]|0.469|
|[[:human genes:s:srp14|SRP14]]|0.437|
|[[:human genes:h:hars|HARS]]|0.425|
|[[:human genes:p:ppp2r3c|PPP2R3C]]|0.406|
|[[:human genes:a:alg1|ALG1]]|0.404|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 430/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|1/1|
|bile duct|15/28|
|blood|11/28|
|bone|21/26|
|breast|18/33|
|central nervous system|39/56|
|cervix|2/4|
|colorectal|9/17|
|esophagus|7/13|
|fibroblast|0/1|
|gastric|6/16|
|kidney|11/21|
|liver|13/20|
|lung|45/75|
|lymphocyte|7/16|
|ovary|13/26|
|pancreas|17/24|
|peripheral nervous system|10/16|
|plasma cell|8/15|
|prostate|0/1|
|skin|18/24|
|soft tissue|6/9|
|thyroid|2/2|
|upper aerodigestive|17/22|
|urinary tract|19/29|
|uterus|3/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 458
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 10.19 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PKM Expression in NALM6 Cells: 10.19'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>