PLK4 [The Human Chemical-Genetic Interaction Map Project]

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======= PLK4 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PLK4
  * **<color #00a2e8>Official Name</color>**: polo like kinase 4
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=10733|10733]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/O00444|O00444]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PLK4&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PLK4|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/605031|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification (PubMed:22020124). {ECO:0000269|PubMed:16244668, ECO:0000269|PubMed:16326102, ECO:0000269|PubMed:17681131, ECO:0000269|PubMed:18239451, ECO:0000269|PubMed:19164942, ECO:0000269|PubMed:21725316, ECO:0000269|PubMed:22020124, ECO:0000269|PubMed:27796307}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|POLO box|
|Pkinase|
|Pkinase Tyr|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|de novo centriole assembly involved in multi-ciliated epithelial cell differentiation|
|deuterosome|
|de novo centriole assembly|
|positive regulation of centriole replication|
|multi-ciliated epithelial cell differentiation|
|positive regulation of centrosome duplication|
|positive regulation of centrosome cycle|
|trophoblast giant cell differentiation|
|XY body|
|centriole replication|
|regulation of centriole replication|
|centriole assembly|
|cell differentiation involved in embryonic placenta development|
|centrosome duplication|
|regulation of centrosome duplication|
|cleavage furrow|
|regulation of centrosome cycle|
|positive regulation of organelle assembly|
|centrosome cycle|
|embryonic placenta development|
|microtubule organizing center organization|
|ciliary basal body-plasma membrane docking|
|G2/M transition of mitotic cell cycle|
|cell cycle G2/M phase transition|
|centriole|
|placenta development|
|organelle localization by membrane tethering|
|membrane docking|
|regulation of microtubule cytoskeleton organization|
|regulation of G2/M transition of mitotic cell cycle|
|regulation of cell cycle G2/M phase transition|
|positive regulation of cytoskeleton organization|
|regulation of organelle assembly|
|regulation of microtubule-based process|
|mitotic cell cycle phase transition|
|cell cycle phase transition|
|positive regulation of cell cycle process|
|cilium assembly|
|protein serine/threonine kinase activity|
|cilium organization|
|in utero embryonic development|
|positive regulation of cell cycle|
|regulation of mitotic cell cycle phase transition|
|reproductive structure development|
|reproductive system development|
|embryonic organ development|
|plasma membrane bounded cell projection assembly|
|regulation of cell cycle phase transition|
|cell projection assembly|
|microtubule cytoskeleton organization|
|centrosome|
|positive regulation of cellular component biogenesis|
|regulation of cytoskeleton organization|
|organelle localization|
|mitotic cell cycle process|
|positive regulation of organelle organization|
|regulation of mitotic cell cycle|
|chordate embryonic development|
|embryo development ending in birth or egg hatching|
|developmental process involved in reproduction|
|microtubule-based process|
|epithelial cell differentiation|
|mitotic cell cycle|
|regulation of cell cycle process|
|organelle assembly|
|nucleolus|
|regulation of cellular component biogenesis|
|protein phosphorylation|
|embryo development|
|cell cycle process|
|identical protein binding|
|cytoskeleton organization|
|epithelium development|
|plasma membrane bounded cell projection organization|
|cell projection organization|
|regulation of cell cycle|
|positive regulation of cellular component organization|
|phosphorylation|
|regulation of organelle organization|
|cell cycle|
|reproductive process|
|reproduction|
|ATP binding|
|tissue development|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp98|BI-6727 0.04μM R03 exp98]]|-2.56|
|[[:results:exp208|Vinblastine 0.015μM R05 exp208]]|-2.27|
|[[:results:exp448|Ammonium tetrathiomolybdate 10μM R08 exp448]]|-2.22|
|[[:results:exp439|QNZ 0.01μM R08 exp439]]|-2.09|
|[[:results:exp507|Monensin 0.3μM R08 exp507]]|-2.06|
|[[:results:exp487|Hinokiflavone 12μM R08 exp487]]|-2.03|
|[[:results:exp244|SB743921 0.001μM R05 exp244]]|-2.03|
|[[:results:exp301|VER-155008 3.9μM R06 exp301]]|-1.94|
|[[:results:exp94|Nocodazole 0.1μM R03 exp94]]|-1.9|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|-1.85|
|[[:results:exp527|Tanespimycin 14μM R08 exp527]]|-1.74|
|[[:results:exp347|Cyclosporin-A 0.8μM R07 exp347]]|1.77|
|[[:results:exp407|Thapsigargin 0.005μM R07 exp407]]|1.77|
|[[:results:exp232|Epothilone-D 0.004 to 0.006μM on day4 R05 exp232]]|1.88|
|[[:results:exp307|Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307]]|1.9|
|[[:results:exp437|Ethanol 0.003 R08 exp437]]|1.9|
|[[:results:exp125|GSK461364A 0.005μM R03 exp125]]|1.97|
|[[:results:exp314|Dimethyloxaloylglycine 11μM R07 exp314]]|2.04|
|[[:results:exp151|SGC0946 7μM R03 exp151]]|2.06|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|2.09|
|[[:results:exp31|Rifampicin 1μM R00 exp31]]|2.1|
|[[:results:exp326|CCT251545 20μM R07 exp326]]|2.14|
|[[:results:exp182|IU1-47 25μM R04 exp182]]|2.15|
|[[:results:exp101|Nicotinamide 1000μM R03 exp101]]|2.23|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|2.27|
|[[:results:exp54|Taxol 0.002μM R01 exp54]]|2.34|
|[[:results:exp425|Beta-Estradiol 0.55μM R07 exp425]]|2.39|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|2.39|
|[[:results:exp15|Cycloheximide 0.2μM R00 exp15]]|2.64|
|[[:results:exp150|SGC0649 7μM R03 exp150]]|3.28|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:t:tfip11|TFIP11]]|0.517|
|[[:human genes:s:stil|STIL]]|0.497|
|[[:human genes:c:cbwd3|CBWD3]]|0.477|
|[[:human genes:c:c14orf93|C14orf93]]|0.475|
|[[:human genes:c:ccdc94|CCDC94]]|0.471|
|[[:human genes:c:cbwd5|CBWD5]]|0.469|
|[[:human genes:w:wdr87|WDR87]]|0.459|
|[[:human genes:c:chd4|CHD4]]|0.458|
|[[:human genes:r:rps3a|RPS3A]]|0.453|
|[[:human genes:p:psmd3|PSMD3]]|0.45|
|[[:human genes:g:golga8o|GOLGA8O]]|0.449|
|[[:human genes:p:psmd11|PSMD11]]|0.448|
|[[:human genes:t:tceb3cl2|TCEB3CL2]]|0.448|
|[[:human genes:p:pcf11|PCF11]]|0.448|
|[[:human genes:a:akirin2|AKIRIN2]]|0.446|
|[[:human genes:c:ccna2|CCNA2]]|0.443|
|[[:human genes:a:arcn1|ARCN1]]|0.441|
|[[:human genes:r:rps12|RPS12]]|0.441|
|[[:human genes:t:tbc1d3g|TBC1D3G]]|0.439|
|[[:human genes:s:ssh2|SSH2]]|0.438|
|[[:human genes:g:gid4|GID4]]|0.436|
|[[:human genes:r:rps19bp1|RPS19BP1]]|0.434|
|[[:human genes:m:myh2|MYH2]]|0.434|
|[[:human genes:e:eif4a3|EIF4A3]]|0.433|
|[[:human genes:n:npipb5|NPIPB5]]|0.431|
|[[:human genes:b:bysl|BYSL]]|0.43|
|[[:human genes:m:mt1e|MT1E]]|0.43|
|[[:human genes:c:cbwd6|CBWD6]]|0.43|
|[[:human genes:c:cox8a|COX8A]]|0.428|
|[[:human genes:s:slc4a1|SLC4A1]]|0.427|
|[[:human genes:o:ogfr|OGFR]]|0.426|
|[[:human genes:i:iscu|ISCU]]|0.425|
|[[:human genes:t:trim52|TRIM52]]|0.425|
|[[:human genes:r:rps2|RPS2]]|0.425|
|[[:human genes:d:ddtl|DDTL]]|0.424|
|[[:human genes:t:tbc1d3|TBC1D3]]|0.424|
|[[:human genes:r:rbm39|RBM39]]|0.423|
|[[:human genes:t:tspy8|TSPY8]]|0.423|
|[[:human genes:c:cdc45|CDC45]]|0.422|
|[[:human genes:g:golga8h|GOLGA8H]]|0.422|
|[[:human genes:s:s1pr4|S1PR4]]|0.422|
|[[:human genes:t:tbc1d3f|TBC1D3F]]|0.422|
|[[:human genes:c:ckap5|CKAP5]]|0.422|
|[[:human genes:c:cdc6|CDC6]]|0.42|
|[[:human genes:c:cmya5|CMYA5]]|0.42|
|[[:human genes:o:orc1|ORC1]]|0.42|
|[[:human genes:s:supt5h|SUPT5H]]|0.419|
|[[:human genes:p:pola1|POLA1]]|0.419|
|[[:human genes:u:usp36|USP36]]|0.418|
|[[:human genes:k:kctd17|KCTD17]]|0.417|
|[[:human genes:m:myt1l|MYT1L]]|0.417|
|[[:human genes:t:tbc1d3h|TBC1D3H]]|0.416|
|[[:human genes:c:casc3|CASC3]]|0.415|
|[[:human genes:c:copg1|COPG1]]|0.413|
|[[:human genes:f:fbxw9|FBXW9]]|0.413|
|[[:human genes:u:ubap1|UBAP1]]|0.412|
|[[:human genes:a:ankle2|ANKLE2]]|0.411|
|[[:human genes:r:rpl10a|RPL10A]]|0.41|
|[[:human genes:n:napa|NAPA]]|0.41|
|[[:human genes:t:ticrr|TICRR]]|0.409|
|[[:human genes:v:vasn|VASN]]|0.408|
|[[:human genes:c:cdca3|CDCA3]]|0.406|
|[[:human genes:p:psmb4|PSMB4]]|0.406|
|[[:human genes:t:tceb3cl|TCEB3CL]]|0.404|
|[[:human genes:r:riok2|RIOK2]]|0.404|
|[[:human genes:g:golga8k|GOLGA8K]]|0.403|
|[[:human genes:a:atp6v1c1|ATP6V1C1]]|0.402|
|[[:human genes:p:paf1|PAF1]]|0.402|
|[[:human genes:c:cdc73|CDC73]]|0.4|
|[[:human genes:s:snrpa1|SNRPA1]]|0.4|
|[[:human genes:o:orc6|ORC6]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 467/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|1/1|
|bile duct|17/28|
|blood|15/28|
|bone|18/26|
|breast|16/33|
|central nervous system|32/56|
|cervix|1/4|
|colorectal|10/17|
|esophagus|12/13|
|fibroblast|1/1|
|gastric|7/16|
|kidney|16/21|
|liver|13/20|
|lung|47/75|
|lymphocyte|11/16|
|ovary|16/26|
|pancreas|17/24|
|peripheral nervous system|14/16|
|plasma cell|6/15|
|prostate|1/1|
|skin|15/24|
|soft tissue|6/9|
|thyroid|1/2|
|upper aerodigestive|17/22|
|urinary tract|20/29|
|uterus|4/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1464
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.93 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PLK4 Expression in NALM6 Cells: 6.93'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>