POLD2 [The Human Chemical-Genetic Interaction Map Project]

This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong.
======= POLD2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: POLD2
  * **<color #00a2e8>Official Name</color>**: DNA polymerase delta 2, accessory subunit
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5425|5425]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P49005|P49005]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=POLD2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20POLD2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/600815|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair (PubMed:12403614, PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200). Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol- delta3 shows higher proofreading activity than Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS as a component of the POLZ complex. Along with POLD3, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (PubMed:24449906). {ECO:0000269|PubMed:12403614, ECO:0000269|PubMed:16510448, ECO:0000269|PubMed:19074196, ECO:0000269|PubMed:20227374, ECO:0000269|PubMed:20334433, ECO:0000269|PubMed:24035200, ECO:0000269|PubMed:24310611, ECO:0000269|PubMed:24449906}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|DNA pol E B|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|delta DNA polymerase complex|
|DNA strand elongation involved in DNA replication|
|DNA strand elongation|
|nucleotide-excision repair, DNA gap filling|
|telomere maintenance via semi-conservative replication|
|DNA-directed DNA polymerase activity|
|mismatch repair|
|nucleotide-excision repair, DNA incision, 5-to lesion|
|DNA damage response, detection of DNA damage|
|nucleotide-excision repair, DNA incision|
|translesion synthesis|
|nuclear DNA replication|
|cell cycle DNA replication|
|DNA synthesis involved in DNA repair|
|postreplication repair|
|transcription-coupled nucleotide-excision repair|
|telomere maintenance|
|telomere organization|
|DNA biosynthetic process|
|nucleotide-excision repair|
|DNA-dependent DNA replication|
|DNA replication|
|nucleic acid phosphodiester bond hydrolysis|
|anatomical structure homeostasis|
|DNA repair|
|detection of stimulus|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|cellular homeostasis|
|cell cycle process|
|chromosome organization|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|organic cyclic compound biosynthetic process|
|cell cycle|
|DNA binding|
|cellular nitrogen compound biosynthetic process|
|homeostatic process|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp531|THZ1 0.06μM R08 exp531]]|-2.69|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|-2.23|
|[[:results:exp513|ONC212 0.15μM R08 exp513]]|-2|
|[[:results:exp451|Atovaquone 15μM R08 exp451]]|-1.96|
|[[:results:exp477|DKK1 89ng/ml R08 exp477]]|-1.93|
|[[:results:exp459|Bleomycin 5μM R08 exp459]]|-1.83|
|[[:results:exp199|Etoposide 0.3μM R05 exp199]]|-1.82|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|-1.77|
|[[:results:exp419|Tunicamycin 0.04 to 0.075μM  on day4 R07 exp419]]|1.76|
|[[:results:exp293|Myriocin 25μM R06 exp293]]|1.82|
|[[:results:exp406|Thalidomide 20μM R07 exp406]]|1.83|
|[[:results:exp248|UM0131023 0.05μM R05 exp248]]|1.86|
|[[:results:exp332|Adefovir 20μM R07 exp332]]|1.87|
|[[:results:exp372|Ibrutinib 1μM R07 exp372]]|1.9|
|[[:results:exp262|Alda-1 10μM R06 exp262]]|1.97|
|[[:results:exp69|Deguelin 0.05μM R02 exp69]]|2.13|
|[[:results:exp506|Momordin-Ic 10μM R08 exp506]]|2.19|
|[[:results:exp335|Aminopterin 0.005μM R07 exp335]]|2.26|
|[[:results:exp285|GW501516 25μM R06 exp285]]|2.27|
|[[:results:exp482|Fas-L 44ng/ml R08 exp482]]|2.28|
|[[:results:exp326|CCT251545 20μM R07 exp326]]|2.38|
|[[:results:exp71|KU-0063794 3.8μM R02 exp71]]|2.45|
|[[:results:exp358|FK-506 5μM R07 exp358]]|3.04|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:s:stil|STIL]]|0.447|
|[[:human genes:k:kctd17|KCTD17]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 166/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|0/1|
|bile duct|5/28|
|blood|8/28|
|bone|8/26|
|breast|6/33|
|central nervous system|16/56|
|cervix|1/4|
|colorectal|5/17|
|esophagus|4/13|
|fibroblast|0/1|
|gastric|4/16|
|kidney|2/21|
|liver|2/20|
|lung|11/75|
|lymphocyte|4/16|
|ovary|7/26|
|pancreas|3/24|
|peripheral nervous system|5/16|
|plasma cell|5/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|2/9|
|thyroid|0/2|
|upper aerodigestive|7/22|
|urinary tract|6/29|
|uterus|3/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 84
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.43 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='POLD2 Expression in NALM6 Cells: 6.43'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>