POLE3 [The Human Chemical-Genetic Interaction Map Project]

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======= POLE3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: POLE3
  * **<color #00a2e8>Official Name</color>**: DNA polymerase epsilon 3, accessory subunit
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=54107|54107]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9NRF9|Q9NRF9]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=POLE3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20POLE3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/607267|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|CBFD NFYB HMF|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|heterochromatin assembly involved in chromatin silencing|
|heterochromatin organization involved in chromatin silencing|
|nucleosome mobilization|
|epsilon DNA polymerase complex|
|heterochromatin assembly|
|heterochromatin organization|
|Ada2/Gcn5/Ada3 transcription activator complex|
|telomere maintenance via semi-conservative replication|
|DNA-directed DNA polymerase activity|
|cellular response to gamma radiation|
|CENP-A containing nucleosome assembly|
|CENP-A containing chromatin organization|
|DNA replication initiation|
|chromatin remodeling at centromere|
|centromere complex assembly|
|DNA replication-independent nucleosome assembly|
|DNA replication-independent nucleosome organization|
|nuclear DNA replication|
|cell cycle DNA replication|
|histone H3 acetylation|
|histone exchange|
|histone acetyltransferase activity|
|response to gamma radiation|
|chromatin organization involved in negative regulation of transcription|
|chromatin silencing|
|cellular response to ionizing radiation|
|chromatin organization involved in regulation of transcription|
|ATP-dependent chromatin remodeling|
|negative regulation of gene expression, epigenetic|
|telomere maintenance|
|telomere organization|
|DNA biosynthetic process|
|histone acetylation|
|nucleosome assembly|
|internal peptidyl-lysine acetylation|
|G1/S transition of mitotic cell cycle|
|cell cycle G1/S phase transition|
|DNA-dependent DNA replication|
|peptidyl-lysine acetylation|
|internal protein amino acid acetylation|
|chromatin assembly|
|protein acetylation|
|response to ionizing radiation|
|gene silencing|
|chromatin assembly or disassembly|
|nucleosome organization|
|chromatin remodeling|
|cellular response to radiation|
|DNA packaging|
|protein acylation|
|protein-DNA complex assembly|
|DNA replication|
|regulation of gene expression, epigenetic|
|protein-DNA complex subunit organization|
|mitotic cell cycle phase transition|
|cell cycle phase transition|
|DNA conformation change|
|peptidyl-lysine modification|
|cellular response to abiotic stimulus|
|cellular response to environmental stimulus|
|anatomical structure homeostasis|
|histone modification|
|covalent chromatin modification|
|response to radiation|
|protein heterodimerization activity|
|mitotic cell cycle process|
|mitotic cell cycle|
|chromatin organization|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|cellular protein-containing complex assembly|
|peptidyl-amino acid modification|
|cellular homeostasis|
|cell cycle process|
|chromosome organization|
|nucleobase-containing compound biosynthetic process|
|response to abiotic stimulus|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|negative regulation of transcription, DNA-templated|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|organic cyclic compound biosynthetic process|
|negative regulation of RNA metabolic process|
|cell cycle|
|negative regulation of cellular macromolecule biosynthetic process|
|negative regulation of nucleobase-containing compound metabolic process|
|DNA binding|
|negative regulation of macromolecule biosynthetic process|
|negative regulation of cellular biosynthetic process|
|negative regulation of biosynthetic process|
|protein-containing complex assembly|
|cellular nitrogen compound biosynthetic process|
|homeostatic process|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|macromolecule biosynthetic process|
|protein-containing complex subunit organization|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp320|ABT-702 5μM plus CoCl2 18μM R07 exp320]]|-2.96|
|[[:results:exp346|CoCl2 18μM R07 exp346]]|-2.42|
|[[:results:exp321|ABT-702 5μM plus Deferoxamine 11μM R07 exp321]]|-2.31|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|-2.08|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|-1.95|
|[[:results:exp472|CI-1040 9.5μM R08 exp472]]|-1.88|
|[[:results:exp19|Etoposide 1μM R00 exp19]]|-1.87|
|[[:results:exp270|Campthothecin 0.001μM R06 exp270]]|-1.81|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|-1.81|
|[[:results:exp227|Cryptotanshinone 12μM R05 exp227]]|-1.78|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|1.74|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|1.74|
|[[:results:exp68|Clomiphene 4.4μM R02 exp68]]|1.85|
|[[:results:exp212|Phenformin 20μM R05 exp212]]|1.91|
|[[:results:exp499|LY2090314 0.003μM R08 exp499]]|2.01|
|[[:results:exp444|THZ531 0.225μM R08 exp444]]|2.23|
|[[:results:exp238|Parthenolide 2.5μM R05 exp238]]|2.26|
|[[:results:exp412|THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412]]|2.27|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:p:pole4|POLE4]]|0.617|
|[[:human genes:c:chtf8|CHTF8]]|0.412|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 38/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|1/28|
|bone|2/25|
|breast|2/33|
|central nervous system|3/56|
|cervix|0/4|
|colorectal|2/17|
|esophagus|0/13|
|fibroblast|1/1|
|gastric|1/15|
|kidney|6/21|
|liver|1/20|
|lung|2/75|
|lymphocyte|1/14|
|ovary|1/26|
|pancreas|1/24|
|peripheral nervous system|1/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|1/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|1/29|
|uterus|1/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1189
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.32 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='POLE3 Expression in NALM6 Cells: 7.32'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>