POLR2A [The Human Chemical-Genetic Interaction Map Project]

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======= POLR2A =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: POLR2A
  * **<color #00a2e8>Official Name</color>**: RNA polymerase II subunit A
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=5430|5430]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P24928|P24928]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=POLR2A&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20POLR2A|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/180660|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors- induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (By similarity). {ECO:0000250|UniProtKB:P08775, ECO:0000269|PubMed:20231364, ECO:0000269|PubMed:23748380, ECO:0000269|PubMed:24207025, ECO:0000269|PubMed:9852112}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|RNA pol Rpb1 4|
|RNA pol Rpb1 2|
|RNA pol Rpb1 7|
|RNA pol Rpb1 5|
|RNA pol Rpb1 6|
|RNA pol Rpb1 3|
|RNA pol Rpb1 1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|transcription, RNA-templated|
|RNA-directed 5-3 RNA polymerase activity|
|RNA polymerase II activity|
|Prp19 complex|
|RNA polymerase II, core complex|
|DNA-directed 5-3 RNA polymerase activity|
|7-methylguanosine mRNA capping|
|positive regulation of RNA splicing|
|RNA capping|
|7-methylguanosine RNA capping|
|positive regulation of viral transcription|
|promoter-specific chromatin binding|
|regulation of viral transcription|
|somatic stem cell population maintenance|
|snRNA transcription by RNA polymerase II|
|snRNA transcription|
|DNA-templated transcription, termination|
|transcription-coupled nucleotide-excision repair|
|transcription elongation from RNA polymerase II promoter|
|fibroblast growth factor receptor signaling pathway|
|regulation of gene silencing by miRNA|
|regulation of gene silencing by RNA|
|regulation of posttranscriptional gene silencing|
|DNA-templated transcription, elongation|
|ncRNA transcription|
|positive regulation of viral process|
|cellular response to fibroblast growth factor stimulus|
|nucleotide-excision repair|
|response to fibroblast growth factor|
|regulation of gene silencing|
|stem cell population maintenance|
|regulation of RNA splicing|
|maintenance of cell number|
|transcription initiation from RNA polymerase II promoter|
|protein C-terminus binding|
|regulation of viral process|
|DNA-templated transcription, initiation|
|regulation of symbiosis, encompassing mutualism through parasitism|
|ubiquitin protein ligase binding|
|mRNA splicing, via spliceosome|
|RNA splicing, via transesterification reactions with bulged adenosine as nucleophile|
|RNA splicing, via transesterification reactions|
|RNA splicing|
|mRNA processing|
|transcription by RNA polymerase II|
|cellular response to growth factor stimulus|
|positive regulation of multi-organism process|
|DNA repair|
|transmembrane receptor protein tyrosine kinase signaling pathway|
|response to growth factor|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|RNA biosynthetic process|
|mRNA metabolic process|
|enzyme linked receptor protein signaling pathway|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|regulation of multi-organism process|
|nucleolus|
|RNA processing|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|cellular response to endogenous stimulus|
|organic cyclic compound biosynthetic process|
|RNA binding|
|DNA binding|
|response to endogenous stimulus|
|cellular nitrogen compound biosynthetic process|
|RNA metabolic process|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|positive regulation of RNA metabolic process|
|macromolecule biosynthetic process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of gene expression|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp2|5-Fluorouracil 20μM R00 exp2]]|-2.31|
|[[:results:exp382|Palbociclib 1μM R07 exp382]]|-1.79|
|[[:results:exp108|Vinblastine 0.2μM R03 exp108]]|-1.73|
|[[:results:exp48|Mubritinib 0.2μM R01 exp48]]|1.76|
|[[:results:exp453|B02 10μM R08 exp453]]|1.81|
|[[:results:exp42|BI-6727 0.001μM R01 exp42]]|1.83|
|[[:results:exp49|NFN1 0.1μM R01 exp49]]|1.94|
|[[:results:exp50|Nicotinamide 2000μM R01 exp50]]|1.96|
|[[:results:exp53|Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53]]|1.99|
|[[:results:exp52|Ribavirin 10μM R01 exp52]]|2.01|
|[[:results:exp263|Aphidicolin 0.04μM R06 exp263]]|2.08|
|[[:results:exp444|THZ531 0.225μM R08 exp444]]|2.08|
|[[:results:exp51|Nifuroxazide 1μM R01 exp51]]|2.11|
|[[:results:exp249|Vinorelbine 0.001μM R05 exp249]]|2.12|
|[[:results:exp12|Chloramphenicol 2μM R00 exp12]]|2.19|
|[[:results:exp60|Vinblastine 0.002μM R01 exp60]]|2.19|
|[[:results:exp282|Fluvastatin 2.2μM R06 exp282]]|2.24|
|[[:results:exp272|CHIR-124 0.04μM R06 exp272]]|2.29|
|[[:results:exp54|Taxol 0.002μM R01 exp54]]|3.35|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:t:tbc1d3f|TBC1D3F]]|0.51|
|[[:human genes:o:osbp|OSBP]]|0.495|
|[[:human genes:t:tceb3c|TCEB3C]]|0.492|
|[[:human genes:s:supt5h|SUPT5H]]|0.488|
|[[:human genes:t:tbc1d3h|TBC1D3H]]|0.488|
|[[:human genes:m:mbtd1|MBTD1]]|0.487|
|[[:human genes:g:golga6l1|GOLGA6L1]]|0.486|
|[[:human genes:s:sf3a3|SF3A3]]|0.483|
|[[:human genes:s:slc6a17|SLC6A17]]|0.482|
|[[:human genes:p:polr2j3|POLR2J3]]|0.471|
|[[:human genes:t:tbc1d3|TBC1D3]]|0.47|
|[[:human genes:e:exosc3|EXOSC3]]|0.468|
|[[:human genes:c:chmp4b|CHMP4B]]|0.466|
|[[:human genes:t:tbc1d3g|TBC1D3G]]|0.465|
|[[:human genes:t:tceb3cl|TCEB3CL]]|0.464|
|[[:human genes:c:cln6|CLN6]]|0.463|
|[[:human genes:a:aatf|AATF]]|0.461|
|[[:human genes:n:nbpf10|NBPF10]]|0.46|
|[[:human genes:c:ccna2|CCNA2]]|0.46|
|[[:human genes:t:tbc1d3c|TBC1D3C]]|0.459|
|[[:human genes:p:psmd3|PSMD3]]|0.458|
|[[:human genes:d:dnttip2|DNTTIP2]]|0.458|
|[[:human genes:c:chd4|CHD4]]|0.455|
|[[:human genes:c:c1qtnf9b|C1QTNF9B]]|0.451|
|[[:human genes:r:rrp7a|RRP7A]]|0.45|
|[[:human genes:k:krt78|KRT78]]|0.444|
|[[:human genes:n:narfl|NARFL]]|0.444|
|[[:human genes:m:mtch2|MTCH2]]|0.443|
|[[:human genes:a:atp6v1c1|ATP6V1C1]]|0.442|
|[[:human genes:d:dtl|DTL]]|0.441|
|[[:human genes:r:rpl14|RPL14]]|0.439|
|[[:human genes:c:cactin|CACTIN]]|0.438|
|[[:human genes:r:rpl35|RPL35]]|0.437|
|[[:human genes:c:copb1|COPB1]]|0.437|
|[[:human genes:p:psmb2|PSMB2]]|0.436|
|[[:human genes:z:znf541|ZNF541]]|0.436|
|[[:human genes:a:ankle2|ANKLE2]]|0.435|
|[[:human genes:t:tspy2|TSPY2]]|0.434|
|[[:human genes:s:snip1|SNIP1]]|0.433|
|[[:human genes:i:ik|IK]]|0.432|
|[[:human genes:c:ctage4|CTAGE4]]|0.428|
|[[:human genes:i:ints6|INTS6]]|0.428|
|[[:human genes:t:tceb3cl2|TCEB3CL2]]|0.427|
|[[:human genes:v:vasn|VASN]]|0.427|
|[[:human genes:r:rps2|RPS2]]|0.426|
|[[:human genes:s:sh3bp1|SH3BP1]]|0.426|
|[[:human genes:x:xylt2|XYLT2]]|0.426|
|[[:human genes:a:atp6v1a|ATP6V1A]]|0.425|
|[[:human genes:r:rrn3|RRN3]]|0.425|
|[[:human genes:r:rps19bp1|RPS19BP1]]|0.422|
|[[:human genes:p:prpf19|PRPF19]]|0.422|
|[[:human genes:r:rpl7l1|RPL7L1]]|0.421|
|[[:human genes:p:psmc2|PSMC2]]|0.421|
|[[:human genes:d:dock6|DOCK6]]|0.421|
|[[:human genes:p:psmb4|PSMB4]]|0.421|
|[[:human genes:s:supt6h|SUPT6H]]|0.419|
|[[:human genes:o:orc6|ORC6]]|0.419|
|[[:human genes:o:orc1|ORC1]]|0.418|
|[[:human genes:p:plk1|PLK1]]|0.418|
|[[:human genes:t:tbc1d3i|TBC1D3I]]|0.418|
|[[:human genes:s:sap30bp|SAP30BP]]|0.417|
|[[:human genes:t:tbc1d3e|TBC1D3E]]|0.417|
|[[:human genes:t:tbc1d3k|TBC1D3K]]|0.416|
|[[:human genes:t:tbc1d3l|TBC1D3L]]|0.416|
|[[:human genes:r:rrp15|RRP15]]|0.416|
|[[:human genes:p:paf1|PAF1]]|0.415|
|[[:human genes:g:gpx4|GPX4]]|0.414|
|[[:human genes:a:arhgef35|ARHGEF35]]|0.414|
|[[:human genes:m:mat2a|MAT2A]]|0.412|
|[[:human genes:r:ruvbl2|RUVBL2]]|0.41|
|[[:human genes:p:pan2|PAN2]]|0.41|
|[[:human genes:t:tgm5|TGM5]]|0.409|
|[[:human genes:c:cep192|CEP192]]|0.409|
|[[:human genes:w:wdr43|WDR43]]|0.407|
|[[:human genes:a:aqr|AQR]]|0.407|
|[[:human genes:p:psmd7|PSMD7]]|0.407|
|[[:human genes:r:rpl9|RPL9]]|0.406|
|[[:human genes:l:lrr1|LRR1]]|0.405|
|[[:human genes:p:pold1|POLD1]]|0.405|
|[[:human genes:s:slc4a1|SLC4A1]]|0.404|
|[[:human genes:t:tspy1|TSPY1]]|0.403|
|[[:human genes:f:fam72d|FAM72D]]|0.403|
|[[:human genes:w:wdr75|WDR75]]|0.403|
|[[:human genes:r:rpl37a|RPL37A]]|0.402|
|[[:human genes:t:ticrr|TICRR]]|0.401|
|[[:human genes:c:chaf1a|CHAF1A]]|0.401|
|[[:human genes:v:vps28|VPS28]]|0.401|
|[[:human genes:c:cdca3|CDCA3]]|0.401|
|[[:human genes:s:strn|STRN]]|0.401|
|[[:human genes:r:rad51|RAD51]]|0.401|
|[[:human genes:n:nol12|NOL12]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: N/A
^Tissue^Fraction Of Cell Lines Where Essential^
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 312
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.71 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='POLR2A Expression in NALM6 Cells: 8.71'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>