PRKAG2 [The Human Chemical-Genetic Interaction Map Project]

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======= PRKAG2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PRKAG2
  * **<color #00a2e8>Official Name</color>**: protein kinase AMP-activated non-catalytic subunit gamma 2
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=51422|51422]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9UGJ0|Q9UGJ0]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PRKAG2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PRKAG2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/602743|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2015]. 
  * **<color #00a2e8>UniProt Summary</color>**:  AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. {ECO:0000269|PubMed:14722619}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|CBS|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|phosphorylase kinase regulator activity|
|AMP-activated protein kinase activity|
|cAMP-dependent protein kinase regulator activity|
|cAMP-dependent protein kinase inhibitor activity|
|carnitine shuttle|
|AMP binding|
|fatty acid transmembrane transport|
|nucleotide-activated protein kinase complex|
|protein kinase activator activity|
|positive regulation of peptidyl-threonine phosphorylation|
|regulation of fatty acid oxidation|
|ATP biosynthetic process|
|intracellular lipid transport|
|ADP binding|
|regulation of peptidyl-threonine phosphorylation|
|purine ribonucleoside triphosphate biosynthetic process|
|regulation of fatty acid biosynthetic process|
|purine nucleoside triphosphate biosynthetic process|
|sterol biosynthetic process|
|glycogen metabolic process|
|cellular glucan metabolic process|
|glucan metabolic process|
|ribonucleoside triphosphate biosynthetic process|
|regulation of glucose import|
|long-chain fatty acid transport|
|purine ribonucleoside triphosphate metabolic process|
|energy reserve metabolic process|
|nucleoside triphosphate biosynthetic process|
|ribonucleoside triphosphate metabolic process|
|purine nucleoside triphosphate metabolic process|
|regulation of glucose transmembrane transport|
|fatty acid transport|
|cellular polysaccharide metabolic process|
|regulation of glycolytic process|
|polysaccharide metabolic process|
|regulation of fatty acid metabolic process|
|regulation of carbohydrate catabolic process|
|nucleoside triphosphate metabolic process|
|mitochondrial transmembrane transport|
|regulation of purine nucleotide metabolic process|
|regulation of nucleotide metabolic process|
|fatty acid biosynthetic process|
|regulation of ATP metabolic process|
|steroid biosynthetic process|
|carboxylic acid transmembrane transport|
|organic acid transmembrane transport|
|negative regulation of protein serine/threonine kinase activity|
|sterol metabolic process|
|monocarboxylic acid transport|
|cell cycle arrest|
|purine ribonucleotide biosynthetic process|
|purine nucleotide biosynthetic process|
|regulation of generation of precursor metabolites and energy|
|ribonucleotide biosynthetic process|
|macroautophagy|
|ribose phosphate biosynthetic process|
|cellular carbohydrate metabolic process|
|purine-containing compound biosynthetic process|
|regulation of cellular ketone metabolic process|
|regulation of macroautophagy|
|regulation of signal transduction by p53 class mediator|
|organic hydroxy compound biosynthetic process|
|monocarboxylic acid biosynthetic process|
|regulation of lipid biosynthetic process|
|ATP metabolic process|
|regulation of carbohydrate metabolic process|
|mitochondrial transport|
|negative regulation of protein kinase activity|
|energy derivation by oxidation of organic compounds|
|nucleotide biosynthetic process|
|negative regulation of kinase activity|
|nucleoside phosphate biosynthetic process|
|steroid metabolic process|
|process utilizing autophagic mechanism|
|autophagy|
|negative regulation of transferase activity|
|anion transmembrane transport|
|carboxylic acid transport|
|organic acid transport|
|lipid transport|
|carboxylic acid biosynthetic process|
|organic acid biosynthetic process|
|purine ribonucleotide metabolic process|
|lipid localization|
|fatty acid metabolic process|
|ribonucleotide metabolic process|
|activation of protein kinase activity|
|purine nucleotide metabolic process|
|regulation of autophagy|
|ribose phosphate metabolic process|
|purine-containing compound metabolic process|
|regulation of lipid metabolic process|
|negative regulation of protein phosphorylation|
|generation of precursor metabolites and energy|
|regulation of small molecule metabolic process|
|negative regulation of phosphorylation|
|nucleotide metabolic process|
|nucleoside phosphate metabolic process|
|organic hydroxy compound metabolic process|
|organic anion transport|
|protein kinase binding|
|carbohydrate metabolic process|
|drug metabolic process|
|regulation of protein serine/threonine kinase activity|
|monocarboxylic acid metabolic process|
|positive regulation of protein kinase activity|
|organophosphate biosynthetic process|
|nucleobase-containing small molecule metabolic process|
|negative regulation of phosphate metabolic process|
|negative regulation of phosphorus metabolic process|
|regulation of transmembrane transport|
|negative regulation of cell cycle|
|positive regulation of kinase activity|
|lipid biosynthetic process|
|anion transport|
|small molecule biosynthetic process|
|negative regulation of protein modification process|
|carbohydrate derivative biosynthetic process|
|positive regulation of transferase activity|
|negative regulation of catalytic activity|
|regulation of protein kinase activity|
|regulation of cellular catabolic process|
|regulation of kinase activity|
|organophosphate metabolic process|
|carboxylic acid metabolic process|
|ion transmembrane transport|
|cellular lipid metabolic process|
|oxidation-reduction process|
|protein phosphorylation|
|regulation of transferase activity|
|regulation of catabolic process|
|cell cycle process|
|oxoacid metabolic process|
|positive regulation of protein phosphorylation|
|organic acid metabolic process|
|carbohydrate derivative metabolic process|
|negative regulation of cellular protein metabolic process|
|positive regulation of phosphorylation|
|nucleobase-containing compound biosynthetic process|
|negative regulation of protein metabolic process|
|positive regulation of phosphate metabolic process|
|positive regulation of phosphorus metabolic process|
|negative regulation of molecular function|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|regulation of cell cycle|
|lipid metabolic process|
|positive regulation of protein modification process|
|transmembrane transport|
|phosphorylation|
|organic cyclic compound biosynthetic process|
|cell cycle|
|ion transport|
|organonitrogen compound biosynthetic process|
|positive regulation of catalytic activity|
|regulation of protein phosphorylation|
|ATP binding|
|intracellular transport|
|regulation of phosphorylation|
|extracellular space|
|positive regulation of cellular protein metabolic process|
|cellular nitrogen compound biosynthetic process|
|intracellular signal transduction|
|positive regulation of protein metabolic process|
|small molecule metabolic process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|cellular catabolic process|
|regulation of intracellular signal transduction|
|establishment of localization in cell|
|regulation of protein modification process|
|regulation of transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp421|VHL-ligand-1 20μM R07 exp421]]|-1.77|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|1.76|
|[[:results:exp308|Rapamycin 2μM plus FK-506 5μM R07 exp308]]|1.8|
|[[:results:exp433|LJH685 50μM R08 exp433]]|1.81|
|[[:results:exp374|Latrunculin-B 10μM R07 exp374]]|2.11|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 11352
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 2.84 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PRKAG2 Expression in NALM6 Cells: 2.84'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>