ROCK1 [The Human Chemical-Genetic Interaction Map Project]

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======= ROCK1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: ROCK1
  * **<color #00a2e8>Official Name</color>**: Rho associated coiled-coil containing protein kinase 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=6093|6093]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q13464|Q13464]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=ROCK1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20ROCK1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/601702|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. {ECO:0000269|PubMed:10436159, ECO:0000269|PubMed:10652353, ECO:0000269|PubMed:11018042, ECO:0000269|PubMed:11283607, ECO:0000269|PubMed:17158456, ECO:0000269|PubMed:18573880, ECO:0000269|PubMed:18694941, ECO:0000269|PubMed:19036714, ECO:0000269|PubMed:19131646, ECO:0000269|PubMed:19181962, ECO:0000269|PubMed:19997641, ECO:0000269|PubMed:21072057, ECO:0000269|PubMed:8617235, ECO:0000269|PubMed:9722579}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|PH|
|Rho Binding|
|HR1|
|Pkinase Tyr|
|Pkinase|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|amyloid-beta complex|
|regulation of angiotensin-activated signaling pathway|
|Rho-dependent protein serine/threonine kinase activity|
|positive regulation of connective tissue replacement|
|apical constriction|
|regulation of connective tissue replacement|
|negative regulation of bicellular tight junction assembly|
|membrane to membrane docking|
|negative regulation of myosin-light-chain-phosphatase activity|
|positive regulation of amyloid-beta clearance|
|regulation of myosin-light-chain-phosphatase activity|
|negative regulation of membrane protein ectodomain proteolysis|
|bleb|
|bleb assembly|
|neuron projection arborization|
|negative regulation of amyloid-beta formation|
|myoblast migration|
|regulation of establishment of endothelial barrier|
|regulation of endothelial cell development|
|negative regulation of amyloid precursor protein catabolic process|
|regulation of amyloid-beta clearance|
|regulation of synaptic vesicle endocytosis|
|leukocyte tethering or rolling|
|leukocyte adhesion to vascular endothelial cell|
|regulation of bicellular tight junction assembly|
|regulation of establishment of cell polarity|
|GTP-Rho binding|
|muscle cell migration|
|tau-protein kinase activity|
|negative regulation of cell junction assembly|
|regulation of membrane protein ectodomain proteolysis|
|regulation of synaptic vesicle recycling|
|regulation of establishment or maintenance of cell polarity|
|positive regulation of focal adhesion assembly|
|response to angiotensin|
|regulation of amyloid-beta formation|
|negative regulation of biomineralization|
|aortic valve morphogenesis|
|negative regulation of biomineral tissue development|
|positive regulation of cardiac muscle hypertrophy|
|mRNA destabilization|
|positive regulation of muscle hypertrophy|
|positive regulation of tissue remodeling|
|positive regulation of adherens junction organization|
|RNA destabilization|
|aortic valve development|
|regulation of amyloid precursor protein catabolic process|
|regulation of endothelial cell differentiation|
|positive regulation of cell junction assembly|
|epithelial cell morphogenesis|
|semi-lunar valve development|
|cellular extravasation|
|regulation of keratinocyte differentiation|
|cortical actin cytoskeleton organization|
|tau protein binding|
|cortical cytoskeleton organization|
|positive regulation of mRNA catabolic process|
|leukocyte cell-cell adhesion|
|positive regulation of cell-matrix adhesion|
|heart valve morphogenesis|
|smooth muscle contraction|
|regulation of cardiac muscle hypertrophy|
|regulation of muscle hypertrophy|
|regulation of focal adhesion assembly|
|regulation of cell-substrate junction assembly|
|negative regulation of phosphoprotein phosphatase activity|
|regulation of epidermal cell differentiation|
|heart valve development|
|negative regulation of protein dephosphorylation|
|cytoplasmic stress granule|
|regulation of adherens junction organization|
|vascular endothelial growth factor receptor signaling pathway|
|mitotic cytokinesis|
|I-kappaB kinase/NF-kappaB signaling|
|regulation of tissue remodeling|
|positive regulation of mRNA metabolic process|
|Rho protein signal transduction|
|regulation of stress fiber assembly|
|peptidyl-threonine phosphorylation|
|regulation of muscle adaptation|
|regulation of epidermis development|
|ephrin receptor signaling pathway|
|regulation of actomyosin structure organization|
|regulation of cell junction assembly|
|actin-mediated cell contraction|
|peptidyl-threonine modification|
|ruffle|
|regulation of actin filament bundle assembly|
|regulation of biomineral tissue development|
|regulation of biomineralization|
|cytoskeleton-dependent cytokinesis|
|cytokinesis|
|negative regulation of angiogenesis|
|negative regulation of protein binding|
|negative regulation of blood vessel morphogenesis|
|negative regulation of phosphatase activity|
|actin filament-based movement|
|negative regulation of dephosphorylation|
|regulation of synaptic vesicle cycle|
|regulation of cell-matrix adhesion|
|actomyosin structure organization|
|secretory granule lumen|
|negative regulation of vasculature development|
|regulation of phosphoprotein phosphatase activity|
|positive regulation of autophagy|
|positive regulation of cell-substrate adhesion|
|negative regulation of translation|
|negative regulation of protein catabolic process|
|regulation of blood vessel diameter|
|regulation of tube diameter|
|regulation of protein dephosphorylation|
|regulation of tube size|
|regulation of epithelial cell differentiation|
|centriole|
|regulation of G protein-coupled receptor signaling pathway|
|negative regulation of neuron apoptotic process|
|negative regulation of cellular amide metabolic process|
|response to transforming growth factor beta|
|vascular process in circulatory system|
|negative regulation of binding|
|peptidyl-serine phosphorylation|
|regulation of phosphatase activity|
|regulation of mRNA stability|
|lamellipodium|
|membrane docking|
|protein localization to plasma membrane|
|regulation of RNA stability|
|epithelial cell development|
|peptidyl-serine modification|
|regulation of mRNA catabolic process|
|regulation of endocytosis|
|negative regulation of neuron death|
|regulation of neuron apoptotic process|
|regulation of cell-substrate adhesion|
|regulation of dephosphorylation|
|regulation of protein binding|
|protein localization to cell periphery|
|regulation of muscle system process|
|protein kinase activity|
|Ras protein signal transduction|
|muscle contraction|
|negative regulation of cellular catabolic process|
|regulation of actin filament organization|
|regulation of angiogenesis|
|muscle system process|
|negative regulation of catabolic process|
|regulation of neuron death|
|small GTPase mediated signal transduction|
|regulation of vasculature development|
|regulation of mRNA metabolic process|
|regulation of autophagy|
|regulation of actin cytoskeleton organization|
|regulation of supramolecular fiber organization|
|negative regulation of proteolysis|
|regulation of translation|
|protein serine/threonine kinase activity|
|positive regulation of cellular catabolic process|
|cytoskeleton|
|regulation of binding|
|leukocyte migration|
|regulation of protein catabolic process|
|regulation of actin filament-based process|
|blood circulation|
|response to peptide hormone|
|circulatory system process|
|positive regulation of cell adhesion|
|regulation of cellular amide metabolic process|
|positive regulation of catabolic process|
|plasma membrane bounded cell projection assembly|
|negative regulation of hydrolase activity|
|cell projection assembly|
|response to peptide|
|protein localization to membrane|
|neuron projection morphogenesis|
|plasma membrane bounded cell projection morphogenesis|
|neutrophil degranulation|
|cell projection morphogenesis|
|neutrophil activation involved in immune response|
|cell division|
|actin cytoskeleton organization|
|neutrophil mediated immunity|
|neutrophil activation|
|granulocyte activation|
|cell-cell adhesion|
|transmembrane receptor protein tyrosine kinase signaling pathway|
|leukocyte degranulation|
|cell part morphogenesis|
|regulation of anatomical structure size|
|myeloid leukocyte mediated immunity|
|positive regulation of cellular component biogenesis|
|myeloid cell activation involved in immune response|
|posttranscriptional regulation of gene expression|
|heart development|
|response to growth factor|
|regulation of cytoskeleton organization|
|positive regulation of MAPK cascade|
|regulation of vesicle-mediated transport|
|cell morphogenesis involved in differentiation|
|negative regulation of phosphate metabolic process|
|negative regulation of phosphorus metabolic process|
|embryonic morphogenesis|
|actin filament-based process|
|myeloid leukocyte activation|
|regulation of system process|
|negative regulation of protein modification process|
|mitotic cell cycle process|
|Golgi membrane|
|leukocyte activation involved in immune response|
|cell activation involved in immune response|
|regulation of neuron differentiation|
|neuron projection development|
|epithelial cell differentiation|
|regulation of cell adhesion|
|mitotic cell cycle|
|regulated exocytosis|
|negative regulation of cellular component organization|
|enzyme linked receptor protein signaling pathway|
|cell morphogenesis|
|regulation of proteolysis|
|regulation of MAPK cascade|
|leukocyte mediated immunity|
|negative regulation of catalytic activity|
|exocytosis|
|neuron development|
|regulation of neurogenesis|
|cellular component morphogenesis|
|regulation of cellular catabolic process|
|circulatory system development|
|peptidyl-amino acid modification|
|negative regulation of apoptotic process|
|negative regulation of programmed cell death|
|response to hormone|
|regulation of cell motility|
|regulation of cellular localization|
|apoptotic process|
|regulation of nervous system development|
|leukocyte activation|
|cell adhesion|
|regulation of cell development|
|biological adhesion|
|negative regulation of developmental process|
|regulation of cellular component biogenesis|
|cell migration|
|protein phosphorylation|
|embryo development|
|regulation of locomotion|
|negative regulation of cell death|
|regulation of catabolic process|
|regulation of cellular component movement|
|cell cycle process|
|response to organonitrogen compound|
|secretion by cell|
|neuron differentiation|
|positive regulation of protein phosphorylation|
|positive regulation of intracellular signal transduction|
|negative regulation of cellular protein metabolic process|
|export from cell|
|programmed cell death|
|positive regulation of phosphorylation|
|regulation of anatomical structure morphogenesis|
|cell activation|
|cell motility|
|localization of cell|
|response to nitrogen compound|
|immune effector process|
|cell death|
|negative regulation of protein metabolic process|
|cytoskeleton organization|
|epithelium development|
|plasma membrane bounded cell projection organization|
|secretion|
|positive regulation of phosphorus metabolic process|
|positive regulation of phosphate metabolic process|
|negative regulation of molecular function|
|cell projection organization|
|negative regulation of multicellular organismal process|
|positive regulation of cellular component organization|
|positive regulation of protein modification process|
|regulation of hydrolase activity|
|phosphorylation|
|regulation of organelle organization|
|locomotion|
|G protein-coupled receptor signaling pathway|
|cell cycle|
|negative regulation of cellular macromolecule biosynthetic process|
|regulation of protein phosphorylation|
|negative regulation of macromolecule biosynthetic process|
|response to endogenous stimulus|
|ATP binding|
|negative regulation of cellular biosynthetic process|
|generation of neurons|
|regulation of apoptotic process|
|negative regulation of biosynthetic process|
|movement of cell or subcellular component|
|response to oxygen-containing compound|
|regulation of programmed cell death|
|cellular protein localization|
|regulation of phosphorylation|
|cellular macromolecule localization|
|positive regulation of cellular protein metabolic process|
|neurogenesis|
|cell development|
|positive regulation of signal transduction|
|regulation of cell death|
|intracellular signal transduction|
|positive regulation of protein metabolic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of multicellular organismal process|
|tissue development|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of cell differentiation|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|regulation of protein modification process|
|regulation of transport|
|immune response|
|positive regulation of nucleobase-containing compound metabolic process|
|extracellular region|
|vesicle-mediated transport|
|system process|
|positive regulation of gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp223|Cabazitaxel 0.001μM R05 exp223]]|-1.96|
|[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|-1.92|
|[[:results:exp231|Epothilone-B 0.0015μM R05 exp231]]|-1.82|
|[[:results:exp45|Docetaxel 0.002μM R01 exp45]]|-1.77|
|[[:results:exp326|CCT251545 20μM R07 exp326]]|-1.71|
|[[:results:exp34|Rotenone 20μM R00 exp34]]|1.99|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 9717
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.9 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='ROCK1 Expression in NALM6 Cells: 7.9'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>