RPA2 [The Human Chemical-Genetic Interaction Map Project]

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======= RPA2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: RPA2
  * **<color #00a2e8>Official Name</color>**: replication protein A2
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=6118|6118]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P15927|P15927]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=RPA2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20RPA2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/179836|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. {ECO:0000269|PubMed:15205463, ECO:0000269|PubMed:17765923, ECO:0000269|PubMed:17959650, ECO:0000269|PubMed:19116208, ECO:0000269|PubMed:20154705, ECO:0000269|PubMed:21504906, ECO:0000269|PubMed:2406247, ECO:0000269|PubMed:24332808, ECO:0000269|PubMed:7697716, ECO:0000269|PubMed:7700386, ECO:0000269|PubMed:8702565, ECO:0000269|PubMed:9430682, ECO:0000269|PubMed:9765279}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|RPA C|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|G-rich strand telomeric DNA binding|
|DNA replication factor A complex|
|regulation of DNA damage checkpoint|
|error-free translesion synthesis|
|error-prone translesion synthesis|
|nucleotide-excision repair, preincision complex stabilization|
|nucleotide-excision repair, DNA incision, 3-to lesion|
|nucleotide-excision repair, DNA gap filling|
|telomere maintenance via semi-conservative replication|
|nucleotide-excision repair, preincision complex assembly|
|regulation of cell cycle checkpoint|
|mismatch repair|
|nucleotide-excision repair, DNA incision, 5-to lesion|
|DNA damage response, detection of DNA damage|
|base-excision repair|
|nucleotide-excision repair, DNA incision|
|translesion synthesis|
|nuclear DNA replication|
|cell cycle DNA replication|
|regulation of double-strand break repair via homologous recombination|
|chromosome, telomeric region|
|DNA synthesis involved in DNA repair|
|interstrand cross-link repair|
|postreplication repair|
|damaged DNA binding|
|site of double-strand break|
|protein localization to chromosome|
|mitotic G1/S transition checkpoint|
|mitotic G1 DNA damage checkpoint|
|G1 DNA damage checkpoint|
|transcription-coupled nucleotide-excision repair|
|regulation of double-strand break repair|
|regulation of cellular response to heat|
|protein phosphatase binding|
|mitotic DNA damage checkpoint|
|double-strand break repair via homologous recombination|
|telomere maintenance|
|recombinational repair|
|PML body|
|telomere organization|
|negative regulation of G1/S transition of mitotic cell cycle|
|regulation of DNA recombination|
|mitotic DNA integrity checkpoint|
|negative regulation of cell cycle G1/S phase transition|
|protein N-terminus binding|
|single-stranded DNA binding|
|nuclear chromosome, telomeric region|
|DNA biosynthetic process|
|nucleotide-excision repair|
|chromatin|
|G1/S transition of mitotic cell cycle|
|DNA-dependent DNA replication|
|cell cycle G1/S phase transition|
|regulation of DNA repair|
|DNA damage checkpoint|
|DNA integrity checkpoint|
|regulation of G1/S transition of mitotic cell cycle|
|mitotic cell cycle checkpoint|
|regulation of cell cycle G1/S phase transition|
|double-strand break repair|
|regulation of signal transduction by p53 class mediator|
|cell cycle checkpoint|
|protein-DNA complex assembly|
|negative regulation of mitotic cell cycle phase transition|
|DNA replication|
|regulation of response to DNA damage stimulus|
|DNA recombination|
|negative regulation of cell cycle phase transition|
|protein-DNA complex subunit organization|
|positive regulation of protein complex assembly|
|mitotic cell cycle phase transition|
|cell cycle phase transition|
|nuclear body|
|ubiquitin protein ligase binding|
|nucleic acid phosphodiester bond hydrolysis|
|negative regulation of mitotic cell cycle|
|negative regulation of cell cycle process|
|anatomical structure homeostasis|
|enzyme binding|
|regulation of DNA metabolic process|
|regulation of mitotic cell cycle phase transition|
|regulation of cell cycle phase transition|
|regulation of protein complex assembly|
|DNA repair|
|positive regulation of cellular component biogenesis|
|negative regulation of cell cycle|
|mitotic cell cycle process|
|regulation of mitotic cell cycle|
|mitotic cell cycle|
|detection of stimulus|
|protein localization to organelle|
|regulation of cellular response to stress|
|DNA metabolic process|
|regulation of cell cycle process|
|cellular response to DNA damage stimulus|
|cellular protein-containing complex assembly|
|cellular homeostasis|
|regulation of cellular component biogenesis|
|cell cycle process|
|chromosome organization|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|regulation of cell cycle|
|positive regulation of cellular component organization|
|organic cyclic compound biosynthetic process|
|cell cycle|
|regulation of response to stress|
|protein-containing complex assembly|
|cellular protein localization|
|cellular macromolecule localization|
|cellular nitrogen compound biosynthetic process|
|homeostatic process|
|cellular response to stress|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|regulation of intracellular signal transduction|
|protein-containing complex subunit organization|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp299|Talazoparib 0.006μM R06 exp299]]|-1.71|
|[[:results:exp37|Wortmannin 0.5μM R00 exp37]]|1.72|
|[[:results:exp27|Pimelic-diphenylamide-106 0.5μM R00 exp27]]|2|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:c:cdc73|CDC73]]|0.497|
|[[:human genes:p:psma6|PSMA6]]|0.475|
|[[:human genes:d:dtl|DTL]]|0.468|
|[[:human genes:t:tbc1d3h|TBC1D3H]]|0.466|
|[[:human genes:t:traip|TRAIP]]|0.454|
|[[:human genes:p:pola2|POLA2]]|0.446|
|[[:human genes:r:rpl35|RPL35]]|0.44|
|[[:human genes:t:tbc1d3k|TBC1D3K]]|0.432|
|[[:human genes:t:tbc1d3i|TBC1D3I]]|0.432|
|[[:human genes:t:tbc1d3e|TBC1D3E]]|0.432|
|[[:human genes:t:tbc1d3l|TBC1D3L]]|0.431|
|[[:human genes:r:rbm39|RBM39]]|0.429|
|[[:human genes:p:polr2j3|POLR2J3]]|0.428|
|[[:human genes:c:col2a1|COL2A1]]|0.424|
|[[:human genes:p:psmb4|PSMB4]]|0.424|
|[[:human genes:e:ebna1bp2|EBNA1BP2]]|0.422|
|[[:human genes:p:prim1|PRIM1]]|0.419|
|[[:human genes:r:rnf113a|RNF113A]]|0.418|
|[[:human genes:p:pan3|PAN3]]|0.418|
|[[:human genes:p:psmd14|PSMD14]]|0.417|
|[[:human genes:p:psmc1|PSMC1]]|0.416|
|[[:human genes:z:zfand5|ZFAND5]]|0.413|
|[[:human genes:p:psmc4|PSMC4]]|0.412|
|[[:human genes:t:tbc1d3f|TBC1D3F]]|0.411|
|[[:human genes:c:chmp4b|CHMP4B]]|0.409|
|[[:human genes:t:tbc1d3c|TBC1D3C]]|0.409|
|[[:human genes:t:tbc1d3|TBC1D3]]|0.408|
|[[:human genes:z:znf106|ZNF106]]|0.407|
|[[:human genes:c:cdc6|CDC6]]|0.407|
|[[:human genes:t:terf1|TERF1]]|0.406|
|[[:human genes:g:golga6l1|GOLGA6L1]]|0.405|
|[[:human genes:d:dido1|DIDO1]]|0.402|
|[[:human genes:t:tyms|TYMS]]|0.402|
|[[:human genes:p:polr1a|POLR1A]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 695/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|25/28|
|bone|24/26|
|breast|29/33|
|central nervous system|53/56|
|cervix|3/4|
|colorectal|16/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|15/16|
|kidney|20/21|
|liver|20/20|
|lung|72/75|
|lymphocyte|13/16|
|ovary|25/26|
|pancreas|22/24|
|peripheral nervous system|16/16|
|plasma cell|14/15|
|prostate|1/1|
|skin|22/24|
|soft tissue|8/9|
|thyroid|1/2|
|upper aerodigestive|21/22|
|urinary tract|29/29|
|uterus|4/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 940
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.18 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='RPA2 Expression in NALM6 Cells: 6.18'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>