SPEN [The Human Chemical-Genetic Interaction Map Project]

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======= SPEN =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: SPEN
  * **<color #00a2e8>Official Name</color>**: spen family transcriptional repressor
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=23013|23013]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q96T58|Q96T58]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=SPEN&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20SPEN|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/613484|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2- mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA. {ECO:0000250, ECO:0000269|PubMed:11331609, ECO:0000269|PubMed:12374742}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|SPOC|
|RRM 1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|transcriptional repressor complex|
|RNA polymerase II transcription factor binding|
|Notch signaling pathway|
|transcription corepressor activity|
|positive regulation of neurogenesis|
|positive regulation of nervous system development|
|positive regulation of cell development|
|viral process|
|symbiotic process|
|regulation of neurogenesis|
|interspecies interaction between organisms|
|negative regulation of transcription by RNA polymerase II|
|regulation of nervous system development|
|regulation of cell development|
|positive regulation of cell differentiation|
|negative regulation of transcription, DNA-templated|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|negative regulation of RNA metabolic process|
|positive regulation of developmental process|
|negative regulation of cellular macromolecule biosynthetic process|
|RNA binding|
|negative regulation of nucleobase-containing compound metabolic process|
|DNA binding|
|negative regulation of macromolecule biosynthetic process|
|negative regulation of cellular biosynthetic process|
|generation of neurons|
|negative regulation of biosynthetic process|
|neurogenesis|
|negative regulation of gene expression|
|positive regulation of multicellular organismal process|
|regulation of cell differentiation|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp352|Dexamethasone 0.006 to 0.015μM on day4 R07 exp352]]|-3.64|
|[[:results:exp75|MK-1775 0.32μM R02 exp75]]|-2.88|
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|-2.71|
|[[:results:exp81|Selumetinib 20μM R02 exp81]]|-2.66|
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|-2.66|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|-2.61|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|-2.59|
|[[:results:exp346|CoCl2 18μM R07 exp346]]|-2.54|
|[[:results:exp365|I-BRD9 4μM R07 exp365]]|-2.51|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|-2.41|
|[[:results:exp38|Wortmannin 5μM R00 exp38]]|-2.26|
|[[:results:exp246|UM0011500 10μM R05 exp246]]|-2.18|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|-2.17|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|-2.1|
|[[:results:exp333|All-trans-Retinoic-Acid 8μM R07 exp333]]|-2.1|
|[[:results:exp37|Wortmannin 0.5μM R00 exp37]]|-2.08|
|[[:results:exp306|Rapamycin 2μM R07 exp306]]|-1.97|
|[[:results:exp245|UM0011500 5μM R05 exp245]]|-1.94|
|[[:results:exp341|BRD2 inhibitor II 20μM R07 exp341]]|-1.9|
|[[:results:exp237|NN-Diethyl-meta-toluamide 100μM R05 exp237]]|-1.9|
|[[:results:exp360|Genistein 15μM R07 exp360]]|-1.88|
|[[:results:exp351|Dexamethasone 0.006μM R07 exp351]]|-1.87|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|-1.87|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|-1.83|
|[[:results:exp229|Dimethyloxaloylglycine 100μM R05 exp229]]|-1.82|
|[[:results:exp432|YM155 0.001μM R08 exp432]]|-1.82|
|[[:results:exp357|Dorsomorphin 5μM R07 exp357]]|-1.81|
|[[:results:exp308|Rapamycin 2μM plus FK-506 5μM R07 exp308]]|-1.81|
|[[:results:exp210|LB-100 2μM R05 exp210]]|-1.81|
|[[:results:exp238|Parthenolide 2.5μM R05 exp238]]|-1.81|
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|-1.78|
|[[:results:exp226|Cerivastatin 0.15μM R05 exp226]]|-1.77|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|-1.77|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|-1.76|
|[[:results:exp23|Nocodazole 0.02μM R00 exp23]]|-1.76|
|[[:results:exp310|2-Methoxyestradiol 0.55μM R07 exp310]]|-1.75|
|[[:results:exp103|Taxol 0.004μM R03 exp103]]|-1.75|
|[[:results:exp211|AICAR 240μM R05 exp211]]|-1.74|
|[[:results:exp227|Cryptotanshinone 12μM R05 exp227]]|-1.73|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|-1.73|
|[[:results:exp135|MS023 7μM R03 exp135]]|1.74|
|[[:results:exp501|Methotrexate 0.01μM R08 exp501]]|1.88|
|[[:results:exp335|Aminopterin 0.005μM R07 exp335]]|1.9|
|[[:results:exp113|1-Methyl-nicotinamide-chloride 1000μM R03 exp113]]|1.96|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:s:sbds|SBDS]]|0.424|
|[[:human genes:s:sap130|SAP130]]|0.422|
|[[:human genes:h:hgc6.3|HGC6.3]]|0.414|
|[[:human genes:j:jmjd6|JMJD6]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 12479
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.36 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='SPEN Expression in NALM6 Cells: 8.36'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>