TCF3 [The Human Chemical-Genetic Interaction Map Project]

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======= TCF3 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: TCF3
  * **<color #00a2e8>Official Name</color>**: transcription factor 3
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=6929|6929]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P15923|P15923]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=TCF3&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20TCF3|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/147141|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Sep 2011]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue- specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E- box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region. {ECO:0000250|UniProtKB:P15806}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|HLH|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|vitamin D response element binding|
|immunoglobulin V(D)J recombination|
|B cell lineage commitment|
|enhancer binding|
|V(D)J recombination|
|mitogen-activated protein kinase kinase kinase binding|
|somatic recombination of immunoglobulin gene segments|
|bHLH transcription factor binding|
|somatic cell DNA recombination|
|somatic diversification of immune receptors via germline recombination within a single locus|
|somatic diversification of immunoglobulins|
|repressing transcription factor binding|
|somatic diversification of immune receptors|
|E-box binding|
|RNA polymerase II transcription factor complex|
|regulation of hematopoietic stem cell differentiation|
|regulation of hematopoietic progenitor cell differentiation|
|positive regulation of muscle cell differentiation|
|B cell differentiation|
|regulation of stem cell differentiation|
|regulation of muscle cell differentiation|
|B cell activation|
|immunoglobulin production|
|production of molecular mediator of immune response|
|transcription factor complex|
|DNA recombination|
|lymphocyte differentiation|
|nuclear chromatin|
|DNA-binding transcription repressor activity, RNA polymerase II-specific|
|cell fate commitment|
|positive regulation of DNA-binding transcription factor activity|
|transcription coactivator activity|
|leukocyte differentiation|
|transcription factor binding|
|positive regulation of neuron differentiation|
|lymphocyte activation|
|nuclear speck|
|regulation of DNA-binding transcription factor activity|
|regulation of hemopoiesis|
|positive regulation of neurogenesis|
|protein heterodimerization activity|
|RNA polymerase II proximal promoter sequence-specific DNA binding|
|positive regulation of nervous system development|
|positive regulation of cell development|
|hemopoiesis|
|hematopoietic or lymphoid organ development|
|transcription, DNA-templated|
|nucleic acid-templated transcription|
|immune system development|
|RNA biosynthetic process|
|regulation of neuron differentiation|
|DNA-binding transcription factor activity|
|DNA metabolic process|
|regulation of neurogenesis|
|negative regulation of transcription by RNA polymerase II|
|protein homodimerization activity|
|regulation of nervous system development|
|leukocyte activation|
|regulation of cell development|
|positive regulation of cell differentiation|
|cell activation|
|nucleobase-containing compound biosynthetic process|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|negative regulation of transcription, DNA-templated|
|positive regulation of transcription by RNA polymerase II|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|organic cyclic compound biosynthetic process|
|negative regulation of RNA metabolic process|
|positive regulation of developmental process|
|negative regulation of cellular macromolecule biosynthetic process|
|negative regulation of nucleobase-containing compound metabolic process|
|DNA binding|
|negative regulation of macromolecule biosynthetic process|
|negative regulation of cellular biosynthetic process|
|generation of neurons|
|positive regulation of transcription, DNA-templated|
|negative regulation of biosynthetic process|
|DNA-binding transcription factor activity, RNA polymerase II-specific|
|neurogenesis|
|cellular nitrogen compound biosynthetic process|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|regulation of immune system process|
|RNA metabolic process|
|cellular macromolecule biosynthetic process|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of multicellular organismal process|
|macromolecule biosynthetic process|
|positive regulation of molecular function|
|regulation of cell differentiation|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp466|Cannabidiol 20μM R08 exp466]]|-3.21|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|-2.97|
|[[:results:exp518|RK-33 8μM R08 exp518]]|-2.94|
|[[:results:exp499|LY2090314 0.003μM R08 exp499]]|-2.93|
|[[:results:exp445|∆-9-Tetrahydrocannabinol 30μM R08 exp445]]|-2.88|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|-2.84|
|[[:results:exp532|TIC10 10μM R08 exp532]]|-2.72|
|[[:results:exp452|Azithromycin 100μM R08 exp452]]|-2.66|
|[[:results:exp3|Actinomycin-D 0.001μM R00 exp3]]|-2.65|
|[[:results:exp334|All-trans-Retinoic-Acid 40μM R07 exp334]]|-2.64|
|[[:results:exp504|MK2206 4μM R08 exp504]]|-2.53|
|[[:results:exp472|CI-1040 9.5μM R08 exp472]]|-2.26|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|-2.26|
|[[:results:exp502|Milciclib 2μM R08 exp502]]|-2.25|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|-2.22|
|[[:results:exp483|FTY720 3μM R08 exp483]]|-2.08|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|-2.07|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|-2.06|
|[[:results:exp433|LJH685 50μM R08 exp433]]|-2.04|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|-2.03|
|[[:results:exp508|NN-Dimethylsphingosine 2.5μM R08 exp508]]|-1.98|
|[[:results:exp435|JQ1 0.8μM R08 exp435]]|-1.97|
|[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|-1.97|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|-1.96|
|[[:results:exp90|WYE-354 6μM R02 exp90]]|-1.95|
|[[:results:exp447|Amiloride 100μM R08 exp447]]|-1.94|
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|-1.94|
|[[:results:exp264|Arsenate 40μM R06 exp264]]|-1.93|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|-1.92|
|[[:results:exp382|Palbociclib 1μM R07 exp382]]|-1.91|
|[[:results:exp459|Bleomycin 5μM R08 exp459]]|-1.9|
|[[:results:exp272|CHIR-124 0.04μM R06 exp272]]|-1.9|
|[[:results:exp28|Pimelic-diphenylamide-106 5μM R00 exp28]]|-1.83|
|[[:results:exp527|Tanespimycin 14μM R08 exp527]]|-1.82|
|[[:results:exp48|Mubritinib 0.2μM R01 exp48]]|-1.8|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|-1.79|
|[[:results:exp503|Mitomycin-C 0.06μM R08 exp503]]|-1.75|
|[[:results:exp470|Chloroquine 32μM R08 exp470]]|-1.74|
|[[:results:exp34|Rotenone 20μM R00 exp34]]|-1.73|
|[[:results:exp80|RO-3307 4.7μM R02 exp80]]|-1.72|
|[[:results:exp292|Menadione 5μM R06 exp292]]|-1.71|
|[[:results:exp114|A-196 10μM R03 exp114]]|1.71|
|[[:results:exp391|Pomalidomide 20μM R07 exp391]]|1.79|
|[[:results:exp11|CCCP 1μM R00 exp11]]|1.84|
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|1.94|
|[[:results:exp351|Dexamethasone 0.006μM R07 exp351]]|2.18|
|[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|2.39|
|[[:results:exp352|Dexamethasone 0.006 to 0.015μM on day4 R07 exp352]]|2.69|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:t:tcf12|TCF12]]|0.495|
|[[:human genes:b:bcor|BCOR]]|0.466|
|[[:human genes:r:rps28|RPS28]]|0.403|
|[[:human genes:m:msi2|MSI2]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 4/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|3/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1398
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 8.6 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='TCF3 Expression in NALM6 Cells: 8.6'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>