UBE2N [The Human Chemical-Genetic Interaction Map Project]

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======= UBE2N =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: UBE2N
  * **<color #00a2e8>Official Name</color>**: ubiquitin conjugating enzyme E2 N
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=7334|7334]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P61088|P61088]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=UBE2N&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20UBE2N|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/603679|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. {ECO:0000269|PubMed:10089880, ECO:0000269|PubMed:14562038, ECO:0000269|PubMed:19269966, ECO:0000269|PubMed:20061386, ECO:0000269|PubMed:21512573}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|UQ con|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|UBC13-UEV1A complex|
|UBC13-MMS2 complex|
|regulation of histone ubiquitination|
|DNA double-strand break processing|
|nucleotide-binding oligomerization domain containing signaling pathway|
|nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway|
|positive regulation of ubiquitin-protein transferase activity|
|cytoplasmic pattern recognition receptor signaling pathway|
|protein K63-linked ubiquitination|
|histone ubiquitination|
|ubiquitin conjugating enzyme activity|
|regulation of ubiquitin-protein transferase activity|
|postreplication repair|
|double-strand break repair via nonhomologous end joining|
|non-recombinational repair|
|positive regulation of DNA repair|
|ubiquitin binding|
|JNK cascade|
|positive regulation of histone modification|
|interleukin-1-mediated signaling pathway|
|double-strand break repair via homologous recombination|
|positive regulation of response to DNA damage stimulus|
|recombinational repair|
|positive regulation of chromatin organization|
|ubiquitin ligase complex|
|stress-activated MAPK cascade|
|positive regulation of protein ubiquitination|
|regulation of DNA repair|
|pattern recognition receptor signaling pathway|
|fibrillar center|
|positive regulation of protein modification by small protein conjugation or removal|
|stress-activated protein kinase signaling cascade|
|regulation of histone modification|
|positive regulation of NF-kappaB transcription factor activity|
|activation of MAPK activity|
|intracellular receptor signaling pathway|
|positive regulation of chromosome organization|
|cellular response to interleukin-1|
|T cell receptor signaling pathway|
|double-strand break repair|
|positive regulation of I-kappaB kinase/NF-kappaB signaling|
|regulation of chromatin organization|
|positive regulation of DNA metabolic process|
|response to interleukin-1|
|regulation of protein ubiquitination|
|regulation of response to DNA damage stimulus|
|DNA recombination|
|regulation of protein modification by small protein conjugation or removal|
|innate immune response-activating signal transduction|
|regulation of I-kappaB kinase/NF-kappaB signaling|
|ubiquitin-protein transferase activity|
|activation of innate immune response|
|positive regulation of MAP kinase activity|
|positive regulation of DNA-binding transcription factor activity|
|ubiquitin protein ligase binding|
|antigen receptor-mediated signaling pathway|
|protein polyubiquitination|
|activation of protein kinase activity|
|positive regulation of protein serine/threonine kinase activity|
|positive regulation of innate immune response|
|regulation of MAP kinase activity|
|regulation of chromosome organization|
|regulation of DNA metabolic process|
|positive regulation of response to biotic stimulus|
|histone modification|
|MAPK cascade|
|covalent chromatin modification|
|signal transduction by protein phosphorylation|
|regulation of DNA-binding transcription factor activity|
|immune response-activating cell surface receptor signaling pathway|
|regulation of innate immune response|
|positive regulation of defense response|
|immune response-regulating cell surface receptor signaling pathway|
|DNA repair|
|positive regulation of multi-organism process|
|regulation of protein serine/threonine kinase activity|
|regulation of response to biotic stimulus|
|ubiquitin-dependent protein catabolic process|
|modification-dependent protein catabolic process|
|positive regulation of protein kinase activity|
|positive regulation of MAPK cascade|
|modification-dependent macromolecule catabolic process|
|immune response-activating signal transduction|
|positive regulation of kinase activity|
|proteolysis involved in cellular protein catabolic process|
|immune response-regulating signaling pathway|
|protein-containing complex|
|positive regulation of response to external stimulus|
|cellular protein catabolic process|
|positive regulation of organelle organization|
|activation of immune response|
|positive regulation of transferase activity|
|cytokine-mediated signaling pathway|
|protein catabolic process|
|protein ubiquitination|
|chromatin organization|
|regulation of cellular response to stress|
|DNA metabolic process|
|regulation of MAPK cascade|
|regulation of defense response|
|protein modification by small protein conjugation|
|regulation of multi-organism process|
|cellular response to DNA damage stimulus|
|regulation of protein kinase activity|
|positive regulation of immune response|
|regulation of kinase activity|
|cellular macromolecule catabolic process|
|protein phosphorylation|
|regulation of transferase activity|
|protein modification by small protein conjugation or removal|
|cellular response to cytokine stimulus|
|positive regulation of protein phosphorylation|
|positive regulation of intracellular signal transduction|
|macromolecule catabolic process|
|positive regulation of phosphorylation|
|organonitrogen compound catabolic process|
|chromosome organization|
|regulation of response to external stimulus|
|response to cytokine|
|positive regulation of phosphate metabolic process|
|positive regulation of phosphorus metabolic process|
|regulation of immune response|
|positive regulation of immune system process|
|positive regulation of cellular component organization|
|positive regulation of protein modification process|
|proteolysis|
|phosphorylation|
|regulation of organelle organization|
|RNA binding|
|positive regulation of catalytic activity|
|regulation of protein phosphorylation|
|regulation of response to stress|
|ATP binding|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|positive regulation of signal transduction|
|regulation of immune system process|
|intracellular signal transduction|
|cellular response to stress|
|positive regulation of protein metabolic process|
|organic substance catabolic process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|cellular catabolic process|
|positive regulation of cell communication|
|positive regulation of signaling|
|regulation of intracellular signal transduction|
|regulation of protein modification process|
|positive regulation of nucleobase-containing compound metabolic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp512|Olaparib 4μM R08 exp512]]|-3.3|
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|-2.88|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|-2.79|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|-2.63|
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|-2.62|
|[[:results:exp525|Sulforaphane 9μM R08 exp525]]|-2.53|
|[[:results:exp518|RK-33 8μM R08 exp518]]|-2.44|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|-2.41|
|[[:results:exp248|UM0131023 0.05μM R05 exp248]]|-2.41|
|[[:results:exp514|Phorbol-12-myristate-13-acetate 0.57μM R08 exp514]]|-2.31|
|[[:results:exp461|BS-181 20μM R08 exp461]]|-2.3|
|[[:results:exp18|Doxycycline 10μM R00 exp18]]|-2.29|
|[[:results:exp533|TNF-alpha 44ng/ml R08 exp533]]|-2.28|
|[[:results:exp134|MS023 2μM R03 exp134]]|-2.27|
|[[:results:exp360|Genistein 15μM R07 exp360]]|-2.19|
|[[:results:exp212|Phenformin 20μM R05 exp212]]|-2.17|
|[[:results:exp155|UNC1999 2μM R03 exp155]]|-2.16|
|[[:results:exp144|PFI-3 10μM R03 exp144]]|-2.14|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|-2.13|
|[[:results:exp335|Aminopterin 0.005μM R07 exp335]]|-2.11|
|[[:results:exp462|Cadmium 60μM R08 exp462]]|-2.11|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|-2.1|
|[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|-2.09|
|[[:results:exp310|2-Methoxyestradiol 0.55μM R07 exp310]]|-2.07|
|[[:results:exp513|ONC212 0.15μM R08 exp513]]|-2.06|
|[[:results:exp41|BI-2536 0.001μM R01 exp41]]|-2.05|
|[[:results:exp107|UMK57 0.6μM R03 exp107]]|-2.05|
|[[:results:exp263|Aphidicolin 0.04μM R06 exp263]]|-2.02|
|[[:results:exp19|Etoposide 1μM R00 exp19]]|-2.02|
|[[:results:exp459|Bleomycin 5μM R08 exp459]]|-2|
|[[:results:exp99|NFN1 0.4μM R03 exp99]]|-2|
|[[:results:exp330|5-Azacytidine 2μM R07 exp330]]|-1.98|
|[[:results:exp199|Etoposide 0.3μM R05 exp199]]|-1.95|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|-1.94|
|[[:results:exp526|Sulforaphane 9μM R08 exp526 no dilution day6]]|-1.92|
|[[:results:exp69|Deguelin 0.05μM R02 exp69]]|-1.91|
|[[:results:exp234|Ethanol 0.01 R05 exp234]]|-1.91|
|[[:results:exp209|Deguelin 0.15μM R05 exp209]]|-1.9|
|[[:results:exp233|EPZ-5676 30μM R05 exp233]]|-1.9|
|[[:results:exp464|Calcipotriol 5μM R08 exp464]]|-1.88|
|[[:results:exp129|Isonicotinamide 500μM R03 exp129]]|-1.88|
|[[:results:exp100|NFN1 1μM R03 exp100]]|-1.86|
|[[:results:exp160|Ribavirin 10 to 15μM on day4 R04 exp160]]|-1.86|
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|-1.84|
|[[:results:exp345|Cidofovir 10μM R07 exp345]]|-1.83|
|[[:results:exp38|Wortmannin 5μM R00 exp38]]|-1.83|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|-1.82|
|[[:results:exp277|Curcumin 6.5μM R06 exp277]]|-1.8|
|[[:results:exp447|Amiloride 100μM R08 exp447]]|-1.79|
|[[:results:exp198|Etoposide 0.1μM R05 exp198]]|-1.75|
|[[:results:exp128|GSK591 2.6μM R03 exp128]]|-1.75|
|[[:results:exp264|Arsenate 40μM R06 exp264]]|-1.75|
|[[:results:exp189|Temozolomide 200μM R04 exp189]]|-1.72|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|-1.71|
|[[:results:exp146|Quinacrine 2.5μM R03 exp146]]|-1.71|
|[[:results:exp446|4-Nitroquinoline-1-oxide 0.5μM R08 exp446]]|-1.7|
|[[:results:exp536|Vitamin-D3 40μM R08 exp536]]|1.82|
|[[:results:exp466|Cannabidiol 20μM R08 exp466]]|2.1|
|[[:results:exp301|VER-155008 3.9μM R06 exp301]]|2.58|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 535/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|1/1|
|bile duct|17/28|
|blood|21/28|
|bone|23/25|
|breast|26/33|
|central nervous system|40/56|
|cervix|2/4|
|colorectal|10/17|
|esophagus|11/13|
|fibroblast|1/1|
|gastric|11/15|
|kidney|11/21|
|liver|11/20|
|lung|58/75|
|lymphocyte|11/14|
|ovary|20/26|
|pancreas|19/24|
|peripheral nervous system|5/16|
|plasma cell|12/15|
|prostate|0/1|
|skin|18/24|
|soft tissue|4/7|
|thyroid|1/2|
|upper aerodigestive|21/22|
|urinary tract|25/29|
|uterus|4/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2252
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.61 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='UBE2N Expression in NALM6 Cells: 6.61'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>