VPS4A [The Human Chemical-Genetic Interaction Map Project]

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======= VPS4A =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: VPS4A
  * **<color #00a2e8>Official Name</color>**: vacuolar protein sorting 4 homolog A
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=27183|27183]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9UN37|Q9UN37]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=VPS4A&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20VPS4A|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/609982|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). {ECO:0000269|PubMed:11563910, ECO:0000269|PubMed:11595185, ECO:0000269|PubMed:15075231, ECO:0000269|PubMed:22660413, ECO:0000269|PubMed:24814515}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|AAA|
|Vps4 C|
|MIT|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|contractile ring contraction|
|actomyosin contractile ring contraction|
|positive regulation of viral budding via host ESCRT complex|
|late endosomal microautophagy|
|regulation of viral budding via host ESCRT complex|
|mitotic cytokinesis checkpoint|
|ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway|
|viral release from host cell|
|exit from host|
|movement in host environment|
|movement in environment of other organism involved in symbiotic interaction|
|actomyosin contractile ring organization|
|abscission|
|exit from host cell|
|vesicle uncoating|
|negative regulation of cytokinesis|
|endosomal vesicle fusion|
|ESCRT complex disassembly|
|ESCRT III complex disassembly|
|positive regulation of viral release from host cell|
|positive regulation of exosomal secretion|
|intracellular cholesterol transport|
|intracellular sterol transport|
|midbody abscission|
|negative regulation of cell division|
|regulation of exosomal secretion|
|nuclear envelope reassembly|
|ATPase activity, coupled|
|mitotic cytokinetic process|
|viral budding via host ESCRT complex|
|protein targeting to lysosome|
|ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway|
|viral budding|
|vacuolar membrane|
|Flemming body|
|multivesicular body assembly|
|regulation of viral release from host cell|
|multivesicular body organization|
|cytokinetic process|
|protein targeting to vacuole|
|intracellular lipid transport|
|protein depolymerization|
|virion assembly|
|protein localization to lysosome|
|cortical actin cytoskeleton organization|
|mitotic metaphase plate congression|
|establishment of protein localization to vacuole|
|cortical cytoskeleton organization|
|nuclear envelope organization|
|cholesterol transport|
|metaphase plate congression|
|positive regulation of viral life cycle|
|protein localization to vacuole|
|sterol transport|
|mitotic cytokinesis|
|vesicle fusion|
|establishment of chromosome localization|
|chromosome localization|
|endosome organization|
|organelle membrane fusion|
|regulation of cytokinesis|
|positive regulation of exocytosis|
|negative regulation of G2/M transition of mitotic cell cycle|
|regulation of protein localization to plasma membrane|
|cytoskeleton-dependent cytokinesis|
|vesicle budding from membrane|
|organelle fusion|
|cytokinesis|
|negative regulation of cell cycle G2/M phase transition|
|lysosomal transport|
|positive regulation of viral process|
|mitotic sister chromatid segregation|
|regulation of protein localization to cell periphery|
|actomyosin structure organization|
|late endosome membrane|
|spindle pole|
|late endosome|
|vacuole organization|
|membrane fusion|
|nucleus organization|
|cellular protein complex disassembly|
|organic hydroxy compound transport|
|vacuolar transport|
|sister chromatid segregation|
|regulation of viral life cycle|
|mitotic nuclear division|
|mitotic cell cycle checkpoint|
|interaction with host|
|midbody|
|macroautophagy|
|regulation of cell division|
|protein C-terminus binding|
|regulation of protein localization to membrane|
|cell cycle checkpoint|
|regulation of G2/M transition of mitotic cell cycle|
|viral life cycle|
|regulation of viral process|
|regulation of cell cycle G2/M phase transition|
|endosome membrane|
|negative regulation of mitotic cell cycle phase transition|
|nuclear chromosome segregation|
|regulation of symbiosis, encompassing mutualism through parasitism|
|regulation of exocytosis|
|endosomal transport|
|negative regulation of cell cycle phase transition|
|ATPase activity|
|protein-containing complex disassembly|
|protein domain specific binding|
|lysosome|
|early endosome|
|autophagy|
|process utilizing autophagic mechanism|
|protein-containing complex binding|
|endosome|
|chromosome segregation|
|nuclear division|
|lipid transport|
|vesicle organization|
|negative regulation of mitotic cell cycle|
|organelle fission|
|lipid localization|
|negative regulation of cell cycle process|
|protein targeting|
|establishment of organelle localization|
|cellular component disassembly|
|positive regulation of secretion by cell|
|regulation of mitotic cell cycle phase transition|
|endomembrane system organization|
|establishment of protein localization to organelle|
|positive regulation of secretion|
|regulation of cell cycle phase transition|
|centrosome|
|cell division|
|actin cytoskeleton organization|
|positive regulation of multi-organism process|
|positive regulation of cellular component biogenesis|
|ubiquitin-dependent protein catabolic process|
|modification-dependent protein catabolic process|
|regulation of cellular protein localization|
|modification-dependent macromolecule catabolic process|
|regulation of vesicle-mediated transport|
|positive regulation of locomotion|
|actin filament-based process|
|negative regulation of cell cycle|
|organelle localization|
|proteolysis involved in cellular protein catabolic process|
|mitotic cell cycle process|
|cellular protein catabolic process|
|regulation of mitotic cell cycle|
|protein catabolic process|
|mitotic cell cycle|
|perinuclear region of cytoplasm|
|viral process|
|protein localization to organelle|
|regulation of secretion by cell|
|regulation of cell cycle process|
|organelle assembly|
|regulation of multi-organism process|
|symbiotic process|
|regulation of secretion|
|interspecies interaction between organisms|
|membrane organization|
|cellular macromolecule catabolic process|
|regulation of cellular localization|
|regulation of cellular component biogenesis|
|regulation of locomotion|
|positive regulation of transport|
|intracellular protein transport|
|cell cycle process|
|regulation of protein localization|
|macromolecule catabolic process|
|organonitrogen compound catabolic process|
|chromosome organization|
|cytoskeleton organization|
|regulation of cell cycle|
|proteolysis|
|locomotion|
|cell cycle|
|ATP binding|
|protein transport|
|intracellular transport|
|peptide transport|
|amide transport|
|cellular protein localization|
|cellular macromolecule localization|
|establishment of protein localization|
|organic substance catabolic process|
|cellular catabolic process|
|establishment of localization in cell|
|nitrogen compound transport|
|protein-containing complex subunit organization|
|regulation of transport|
|vesicle-mediated transport|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp165|RO-3306 3 to 4μM on day4 R04 exp165]]|1.88|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 49/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|1/28|
|blood|0/28|
|bone|0/26|
|breast|5/33|
|central nervous system|1/56|
|cervix|0/4|
|colorectal|1/17|
|esophagus|1/13|
|fibroblast|0/1|
|gastric|1/16|
|kidney|0/21|
|liver|0/20|
|lung|4/75|
|lymphocyte|1/16|
|ovary|3/26|
|pancreas|8/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|4/22|
|urinary tract|4/29|
|uterus|1/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 4836
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.92 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='VPS4A Expression in NALM6 Cells: 6.92'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>