VRK1 [The Human Chemical-Genetic Interaction Map Project]

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======= VRK1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: VRK1
  * **<color #00a2e8>Official Name</color>**: VRK serine/threonine kinase 1
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=7443|7443]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q99986|Q99986]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=VRK1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20VRK1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/602168|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr- 18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity. {ECO:0000269|PubMed:10951572, ECO:0000269|PubMed:14645249, ECO:0000269|PubMed:15105425, ECO:0000269|PubMed:16495336, ECO:0000269|PubMed:18617507, ECO:0000269|PubMed:19103756}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Pkinase|
|Pkinase Tyr|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|histone kinase activity (H3-S10 specific)|
|histone H3-T3 phosphorylation|
|histone kinase activity (H3-T3 specific)|
|nucleosomal histone binding|
|histone H3-S10 phosphorylation|
|Golgi disassembly|
|histone-threonine phosphorylation|
|organelle inheritance|
|mitotic nuclear envelope reassembly|
|Golgi inheritance|
|histone-serine phosphorylation|
|mitotic nuclear envelope disassembly|
|membrane disassembly|
|nuclear envelope disassembly|
|nuclear envelope reassembly|
|histone phosphorylation|
|Golgi stack|
|nuclear envelope organization|
|organelle disassembly|
|peptidyl-threonine phosphorylation|
|peptidyl-threonine modification|
|spindle|
|Golgi organization|
|nucleus organization|
|peptidyl-serine phosphorylation|
|protein autophosphorylation|
|peptidyl-serine modification|
|protein kinase activity|
|protein serine/threonine kinase activity|
|histone modification|
|covalent chromatin modification|
|cellular component disassembly|
|endomembrane system organization|
|protein kinase binding|
|cell division|
|mitotic cell cycle process|
|mitotic cell cycle|
|chromatin organization|
|membrane organization|
|nucleolus|
|peptidyl-amino acid modification|
|protein phosphorylation|
|cell cycle process|
|chromosome organization|
|phosphorylation|
|cell cycle|
|ATP binding|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp233|EPZ-5676 30μM R05 exp233]]|-2.68|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|-2.44|
|[[:results:exp291|LLY-284 2.6μM R06 exp291]]|-2.21|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|-2.14|
|[[:results:exp359|FK-506 30μM R07 exp359]]|-2.06|
|[[:results:exp331|A-769662 20μM R07 exp331]]|-2.05|
|[[:results:exp231|Epothilone-B 0.0015μM R05 exp231]]|-2.04|
|[[:results:exp232|Epothilone-D 0.004 to 0.006μM on day4 R05 exp232]]|-2.03|
|[[:results:exp30|Rapamycin 10μM R00 exp30]]|-2.03|
|[[:results:exp436|Dynasore 7μM R08 exp436]]|-2|
|[[:results:exp306|Rapamycin 2μM R07 exp306]]|-1.97|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|-1.95|
|[[:results:exp241|QNZ 0.01μM R05 exp241]]|-1.91|
|[[:results:exp537|WNT3A 44ng/ml R08 exp537]]|-1.91|
|[[:results:exp399|Salubrinal 20μM R07 exp399]]|-1.9|
|[[:results:exp310|2-Methoxyestradiol 0.55μM R07 exp310]]|-1.87|
|[[:results:exp429|Rapamycin 0.001μM R08 exp429]]|-1.86|
|[[:results:exp225|Celastrol 0.12μM R05 exp225]]|-1.84|
|[[:results:exp358|FK-506 5μM R07 exp358]]|-1.84|
|[[:results:exp238|Parthenolide 2.5μM R05 exp238]]|-1.84|
|[[:results:exp346|CoCl2 18μM R07 exp346]]|-1.83|
|[[:results:exp430|Rifampicin 30μM R08 exp430]]|-1.81|
|[[:results:exp286|HMS-I2 1μM R06 exp286]]|-1.8|
|[[:results:exp372|Ibrutinib 1μM R07 exp372]]|-1.8|
|[[:results:exp289|Hydroxyurea 15μM R06 exp289]]|-1.78|
|[[:results:exp514|Phorbol-12-myristate-13-acetate 0.57μM R08 exp514]]|-1.78|
|[[:results:exp423|Zebularine 20μM R07 exp423]]|-1.78|
|[[:results:exp417|Tubastatin-A 2.5μM R07 exp417]]|-1.77|
|[[:results:exp209|Deguelin 0.15μM R05 exp209]]|-1.75|
|[[:results:exp18|Doxycycline 10μM R00 exp18]]|-1.75|
|[[:results:exp275|Citral 75μM R06 exp275]]|-1.73|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|-1.71|
|[[:results:exp28|Pimelic-diphenylamide-106 5μM R00 exp28]]|2.12|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:h:hars|HARS]]|0.579|
|[[:human genes:g:gtf3c2|GTF3C2]]|0.575|
|[[:human genes:c:cct7|CCT7]]|0.561|
|[[:human genes:r:rtcb|RTCB]]|0.551|
|[[:human genes:a:aars|AARS]]|0.528|
|[[:human genes:g:gmppb|GMPPB]]|0.517|
|[[:human genes:r:rnmt|RNMT]]|0.513|
|[[:human genes:e:eif4e|EIF4E]]|0.509|
|[[:human genes:y:yars|YARS]]|0.506|
|[[:human genes:u:uxt|UXT]]|0.506|
|[[:human genes:r:rptor|RPTOR]]|0.501|
|[[:human genes:u:uap1|UAP1]]|0.5|
|[[:human genes:r:rad1|RAD1]]|0.5|
|[[:human genes:d:dpagt1|DPAGT1]]|0.494|
|[[:human genes:e:eprs|EPRS]]|0.492|
|[[:human genes:n:nae1|NAE1]]|0.491|
|[[:human genes:d:ddx20|DDX20]]|0.49|
|[[:human genes:a:alg1|ALG1]]|0.488|
|[[:human genes:r:rpe|RPE]]|0.487|
|[[:human genes:h:hspd1|HSPD1]]|0.485|
|[[:human genes:p:pmm2|PMM2]]|0.481|
|[[:human genes:s:srp68|SRP68]]|0.479|
|[[:human genes:n:nars|NARS]]|0.475|
|[[:human genes:n:nsmce1|NSMCE1]]|0.472|
|[[:human genes:t:triap1|TRIAP1]]|0.472|
|[[:human genes:o:otud5|OTUD5]]|0.468|
|[[:human genes:t:telo2|TELO2]]|0.466|
|[[:human genes:t:tti2|TTI2]]|0.464|
|[[:human genes:t:tbca|TBCA]]|0.464|
|[[:human genes:r:rpp21|RPP21]]|0.461|
|[[:human genes:p:ppp4c|PPP4C]]|0.46|
|[[:human genes:g:gtf3c3|GTF3C3]]|0.459|
|[[:human genes:a:adsl|ADSL]]|0.457|
|[[:human genes:a:alg5|ALG5]]|0.456|
|[[:human genes:a:alg2|ALG2]]|0.455|
|[[:human genes:m:mars|MARS]]|0.455|
|[[:human genes:g:gnb1l|GNB1L]]|0.453|
|[[:human genes:g:gtf3c6|GTF3C6]]|0.451|
|[[:human genes:z:znf259|ZNF259]]|0.45|
|[[:human genes:g:gemin5|GEMIN5]]|0.45|
|[[:human genes:a:alg13|ALG13]]|0.445|
|[[:human genes:r:rbmx2|RBMX2]]|0.444|
|[[:human genes:c:csnk2b|CSNK2B]]|0.442|
|[[:human genes:l:lamtor5|LAMTOR5]]|0.44|
|[[:human genes:d:dhps|DHPS]]|0.44|
|[[:human genes:g:gfpt1|GFPT1]]|0.439|
|[[:human genes:t:trmt5|TRMT5]]|0.438|
|[[:human genes:s:sec61g|SEC61G]]|0.437|
|[[:human genes:c:ccdc101|CCDC101]]|0.436|
|[[:human genes:p:polr3h|POLR3H]]|0.435|
|[[:human genes:t:tsen2|TSEN2]]|0.435|
|[[:human genes:u:utp23|UTP23]]|0.432|
|[[:human genes:p:polr3b|POLR3B]]|0.432|
|[[:human genes:t:thg1l|THG1L]]|0.429|
|[[:human genes:e:exoc4|EXOC4]]|0.428|
|[[:human genes:d:dohh|DOHH]]|0.428|
|[[:human genes:d:dctn6|DCTN6]]|0.427|
|[[:human genes:m:mtor|MTOR]]|0.425|
|[[:human genes:t:tprkb|TPRKB]]|0.424|
|[[:human genes:c:cbfb|CBFB]]|0.423|
|[[:human genes:h:haus8|HAUS8]]|0.421|
|[[:human genes:b:bap1|BAP1]]|0.42|
|[[:human genes:m:meaf6|MEAF6]]|0.42|
|[[:human genes:s:spcs3|SPCS3]]|0.416|
|[[:human genes:s:spcs2|SPCS2]]|0.416|
|[[:human genes:m:mapk14|MAPK14]]|0.415|
|[[:human genes:t:tti1|TTI1]]|0.415|
|[[:human genes:s:srp14|SRP14]]|0.415|
|[[:human genes:t:taz|TAZ]]|0.413|
|[[:human genes:s:srp72|SRP72]]|0.412|
|[[:human genes:s:slc35b1|SLC35B1]]|0.412|
|[[:human genes:p:pik3r4|PIK3R4]]|0.412|
|[[:human genes:p:pam16|PAM16]]|0.411|
|[[:human genes:a:alg14|ALG14]]|0.409|
|[[:human genes:u:uhrf1|UHRF1]]|0.409|
|[[:human genes:e:eny2|ENY2]]|0.407|
|[[:human genes:h:hccs|HCCS]]|0.404|
|[[:human genes:t:tsen54|TSEN54]]|0.404|
|[[:human genes:a:atxn10|ATXN10]]|0.403|
|[[:human genes:c:casp9|CASP9]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 91/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|6/28|
|blood|0/28|
|bone|8/25|
|breast|5/33|
|central nervous system|23/56|
|cervix|1/4|
|colorectal|1/17|
|esophagus|0/13|
|fibroblast|1/1|
|gastric|0/15|
|kidney|1/21|
|liver|0/20|
|lung|7/75|
|lymphocyte|0/14|
|ovary|2/26|
|pancreas|3/24|
|peripheral nervous system|10/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|2/24|
|soft tissue|0/7|
|thyroid|1/2|
|upper aerodigestive|2/22|
|urinary tract|1/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1586
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 6.46 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='VRK1 Expression in NALM6 Cells: 6.46'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>