WT1 [The Human Chemical-Genetic Interaction Map Project]

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======= WT1 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: WT1
  * **<color #00a2e8>Official Name</color>**: WT1 transcription factor
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=7490|7490]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P19544|P19544]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=WT1&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20WT1|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/607102|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]. 
  * **<color #00a2e8>UniProt Summary</color>**: N/A

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|WT1|
|zf-C2H2|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|negative regulation of metanephric glomerulus development|
|negative regulation of metanephric glomerular mesangial cell proliferation|
|regulation of metanephric ureteric bud development|
|visceral serous pericardium development|
|posterior mesonephric tubule development|
|regulation of metanephric glomerular mesangial cell proliferation|
|positive regulation of metanephric ureteric bud development|
|adrenal cortex formation|
|adrenal cortex development|
|negative regulation of glomerular mesangial cell proliferation|
|negative regulation of glomerulus development|
|hemi-methylated DNA-binding|
|positive regulation of DNA methylation|
|negative regulation of female gonad development|
|thorax and anterior abdomen determination|
|double-stranded methylated DNA binding|
|negative regulation of cell proliferation involved in kidney development|
|metanephric S-shaped body morphogenesis|
|negative regulation of gonad development|
|cardiac muscle cell fate commitment|
|regulation of metanephric glomerulus development|
|zygotic determination of anterior/posterior axis, embryo|
|S-shaped body morphogenesis|
|positive regulation of male gonad development|
|negative regulation of metanephros development|
|diaphragm development|
|regulation of male gonad development|
|glomerular basement membrane development|
|cardiac cell fate commitment|
|regulation of glomerular mesangial cell proliferation|
|positive regulation of gonad development|
|regulation of female gonad development|
|regulation of cell proliferation involved in kidney development|
|metanephric mesenchyme development|
|positive regulation of metanephros development|
|C2H2 zinc finger domain binding|
|tripartite regional subdivision|
|muscle cell fate commitment|
|mesenchymal to epithelial transition|
|regulation of glomerulus development|
|anterior/posterior axis specification, embryo|
|glomerular visceral epithelial cell differentiation|
|negative regulation of kidney development|
|renal filtration cell differentiation|
|glomerular epithelial cell differentiation|
|cellular response to gonadotropin stimulus|
|kidney mesenchyme development|
|regulation of gonad development|
|blastoderm segmentation|
|pericardium development|
|glomerular epithelium development|
|metanephric nephron morphogenesis|
|positive regulation of mesonephros development|
|metanephric epithelium development|
|sex determination|
|male genitalia development|
|regulation of metanephros development|
|regulation of DNA methylation|
|adrenal gland development|
|regulation of mesonephros development|
|metanephros morphogenesis|
|response to gonadotropin|
|epithelial cell differentiation involved in kidney development|
|positive regulation of pri-miRNA transcription by RNA polymerase II|
|metanephric nephron development|
|embryonic axis specification|
|regulation of animal organ formation|
|positive regulation of heart growth|
|positive regulation of kidney development|
|cell differentiation involved in kidney development|
|regulation of pri-miRNA transcription by RNA polymerase II|
|branching involved in ureteric bud morphogenesis|
|genitalia development|
|positive regulation of organ growth|
|anterior/posterior axis specification|
|glomerulus development|
|ureteric bud morphogenesis|
|mesonephric tubule morphogenesis|
|cellular response to cAMP|
|regulation of kidney development|
|negative regulation of reproductive process|
|embryonic pattern specification|
|nephron tubule morphogenesis|
|regulation of heart growth|
|nephron epithelium morphogenesis|
|nephron morphogenesis|
|vasculogenesis|
|renal tubule morphogenesis|
|nephron tubule development|
|positive regulation of reproductive process|
|renal tubule development|
|positive regulation of animal organ morphogenesis|
|metanephros development|
|kidney morphogenesis|
|ureteric bud development|
|axis specification|
|mesonephric tubule development|
|mesonephric epithelium development|
|cardiac muscle cell differentiation|
|mesonephros development|
|regulation of organ growth|
|segmentation|
|nephron epithelium development|
|response to cAMP|
|negative regulation of vasculature development|
|cardiocyte differentiation|
|endocrine system development|
|nephron development|
|kidney epithelium development|
|branching morphogenesis of an epithelial tube|
|skeletal muscle organ development|
|male gonad development|
|development of primary male sexual characteristics|
|response to organophosphorus|
|response to purine-containing compound|
|male sex differentiation|
|morphogenesis of a branching epithelium|
|regulation of reproductive process|
|cardiac muscle tissue development|
|morphogenesis of a branching structure|
|positive regulation of developmental growth|
|negative regulation of cell growth|
|positive regulation of DNA metabolic process|
|respiratory system development|
|striated muscle cell differentiation|
|gonad development|
|development of primary sexual characteristics|
|anterior/posterior pattern specification|
|transcription regulatory region DNA binding|
|mesenchyme development|
|negative regulation of growth|
|muscle cell differentiation|
|cell fate commitment|
|germ cell development|
|regulation of animal organ morphogenesis|
|positive regulation of growth|
|sex differentiation|
|kidney development|
|renal system development|
|striated muscle tissue development|
|muscle organ development|
|muscle tissue development|
|epithelial tube morphogenesis|
|camera-type eye development|
|urogenital system development|
|regulation of vasculature development|
|regulation of developmental growth|
|regionalization|
|extracellular matrix organization|
|regulation of DNA metabolic process|
|cellular process involved in reproduction in multicellular organism|
|eye development|
|visual system development|
|sensory system development|
|extracellular structure organization|
|RNA splicing|
|nuclear speck|
|blood vessel morphogenesis|
|gland development|
|regulation of cell growth|
|sequence-specific DNA binding|
|reproductive structure development|
|reproductive system development|
|morphogenesis of an epithelium|
|pattern specification process|
|DNA-binding transcription activator activity, RNA polymerase II-specific|
|muscle structure development|
|blood vessel development|
|vasculature development|
|cardiovascular system development|
|heart development|
|cellular response to organic cyclic compound|
|sensory organ development|
|tissue morphogenesis|
|cellular response to organonitrogen compound|
|cellular response to hormone stimulus|
|tube morphogenesis|
|cellular response to nitrogen compound|
|developmental process involved in reproduction|
|regulation of growth|
|negative regulation of cell population proliferation|
|DNA-binding transcription factor activity|
|epithelial cell differentiation|
|gamete generation|
|multicellular organismal reproductive process|
|sexual reproduction|
|zinc ion binding|
|multicellular organism reproduction|
|tube development|
|nucleolus|
|negative regulation of transcription by RNA polymerase II|
|circulatory system development|
|RNA processing|
|negative regulation of apoptotic process|
|anatomical structure formation involved in morphogenesis|
|negative regulation of programmed cell death|
|response to hormone|
|response to organic cyclic compound|
|negative regulation of developmental process|
|animal organ morphogenesis|
|embryo development|
|multi-organism reproductive process|
|negative regulation of cell death|
|response to organonitrogen compound|
|cellular response to oxygen-containing compound|
|regulation of anatomical structure morphogenesis|
|response to nitrogen compound|
|epithelium development|
|negative regulation of transcription, DNA-templated|
|negative regulation of multicellular organismal process|
|positive regulation of transcription by RNA polymerase II|
|cellular response to endogenous stimulus|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|negative regulation of RNA metabolic process|
|positive regulation of developmental process|
|negative regulation of cellular macromolecule biosynthetic process|
|RNA binding|
|reproductive process|
|reproduction|
|negative regulation of nucleobase-containing compound metabolic process|
|negative regulation of macromolecule biosynthetic process|
|response to endogenous stimulus|
|negative regulation of cellular biosynthetic process|
|positive regulation of transcription, DNA-templated|
|regulation of apoptotic process|
|negative regulation of biosynthetic process|
|response to oxygen-containing compound|
|DNA-binding transcription factor activity, RNA polymerase II-specific|
|regulation of programmed cell death|
|regulation of cell population proliferation|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|cell development|
|RNA metabolic process|
|regulation of cell death|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of multicellular organismal process|
|tissue development|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|-2.27|
|[[:results:exp170|Metformin 100 to 150μM on day4 R04 exp170]]|-2.14|
|[[:results:exp27|Pimelic-diphenylamide-106 0.5μM R00 exp27]]|-2.07|
|[[:results:exp256|HMS-I1 10μM R06 exp256]]|-1.79|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 1/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|1/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 17368
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 0.37 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='WT1 Expression in NALM6 Cells: 0.37'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>