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Ask your administrator if you think this is wrong. ====== Chloramphenicol 2μM R00 exp12 ====== ==== Mechanism of Action ==== Binds 23S rRNA of 50S ribosomal subunit, inhibits protein synthesis * **<color #00a2e8>Class / Subclass 1:</color>** Infectious Disease / Antibiotic * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Mitochondrial Inhibitor ==== Technical Notes ==== <accordion collapsed='true'> <panel title='Protein References' collapsed='true'> * **<color #00a2e8>PubChem Name:</color>** %%Chloramphenicol%% * **<color #00a2e8>Synonyms:</color>** N/A * **<color #00a2e8>CAS #:</color>** 56-75-7 * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5959|5959]] * **<color #00a2e8>IUPAC:</color>** %%2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide%% * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C11H12Cl2N2O5/c12-10(13)11(18)14-8(5-16)9(17)6-1-3-7(4-2-6)15(19)20/h1-4,8-10,16-17H,5H2,(H,14,18)/t8-,9-/m1/s1 * **<color #00a2e8>INCHI Key:</color>** WIIZWVCIJKGZOK-RKDXNWHRSA-N * **<color #00a2e8>Molecular Weight:</color>** 323.13 * **<color #00a2e8>Canonical SMILES:</color>** C1=CC(=CC=C1C(C(CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-] * **<color #00a2e8>Isomeric SMILES:</color>** C1=CC(=CC=C1[C@H]([C@@H](CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-] * **<color #00a2e8>Molecular Formula:</color>** C11H12Cl2N2O5 {{:chemogenomics:structures:chem-0007.svg?nolink}} </panel> <panel title='Protein Supplier' collapsed='true'> * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich * **<color #00a2e8>Catalog #:</color>** C0378 * **<color #00a2e8>Lot #:</color>** 096K1244 </panel> <panel title='Protein Characterization' collapsed='true'> * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C11H12Cl2N2O5 323.0196; found 323.01899 </panel> <panel title='Dose Response Curve' collapsed='true'> * **<color #00a2e8>Platform ID:</color>** Chloramphenicol * **<color #00a2e8>Min:</color>** -5.6180; **<color #00a2e8>Max:</color>** 8.0817 {{:chemogenomics:dose_response:dr_260.png?nolink&500 |}} \\ \\ \\ \\ |< 300px 100px 200px >| ^ IC ^ Concentration (µM) ^ | IC10 |N/A | | IC20 |N/A | | IC30 |N/A | | IC40 |N/A | | IC50 |N/A | | IC60 |N/A | | IC70 |N/A | | IC80 |N/A | | IC90 |N/A | \\ </panel> </accordion> ==== Screen Summary ==== * **<color #00a2e8>Round</color>**: 00 * **<color #00a2e8>Dose</color>**: 2µM * **<color #00a2e8>Days of incubation</color>**: 8 * **<color #00a2e8>Doublings</color>**: 6.1 * **<color #00a2e8>Numbers of reads</color>**: 12267396 ==== Screen Results ==== ^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^ |0/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Chloramphenicol_2uM_Round-0_exp12.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>| <modal id='ScorePlot' size='lg' title='Chloramphenicol 2μM R00 exp12'> {{:chemogenomics:cranks_plots:exp12.png?nolink}} </modal> <modal id="modal_hits" size="sm" title="Top 30 S/R Genes"> ^Gene^CRANKS Score^FDR^ |[[:human_genes:P:PRAMEF25|PRAMEF25]]|-2.71|0.62| |[[:human_genes:P:PRAMEF26|PRAMEF26]]|-2.71|0.62| |[[:human_genes:Q:QRICH1|QRICH1]]|-2.68|0.17| |[[:human_genes:R:RPP30|RPP30]]|-2.66|0.14| |[[:human_genes:C:CCDC42|CCDC42]]|-2.42|0.18| |[[:human_genes:T:TEN1|TEN1]]|-2.39|0.18| |[[:human_genes:K:KIAA0355|KIAA0355]]|-2.28|0.28| |[[:human_genes:K:KIAA1432|KIAA1432]]|-2.20|0.31| |[[:human_genes:K:KANK4|KANK4]]|-2.20|0.31| |[[:human_genes:D:DDX55|DDX55]]|-2.13|0.52| |[[:human_genes:N:NHLRC2|NHLRC2]]|-2.13|0.52| |[[:human_genes:N:NUDT5|NUDT5]]|-2.12|0.43| |[[:human_genes:F:FAM50A|FAM50A]]|-2.08|0.62| |[[:human_genes:T:TOMM5|TOMM5]]|-2.06|0.52| |[[:human_genes:S:SHISA4|SHISA4]]|-2.02|0.62| |[[:human_genes:G:GPS2|GPS2]]|-2.02|0.55| |[[:human_genes:L:LAMTOR4|LAMTOR4]]|-2.02|0.52| |[[:human_genes:N:NCLN|NCLN]]|-2.01|0.52| |[[:human_genes:P:PRMT5|PRMT5]]|-2.01|0.63| |[[:human_genes:A:AHCY|AHCY]]|-1.99|0.52| |[[:human_genes:C:CTC1|CTC1]]|-1.98|0.52| |[[:human_genes:T:TAF1C|TAF1C]]|-1.98|0.52| |[[:human_genes:G:GUCA2B|GUCA2B]]|-1.98|0.52| |[[:human_genes:O:ORC3|ORC3]]|-1.97|0.61| |[[:human_genes:F:FOXL2|FOXL2]]|-1.97|0.52| |[[:human_genes:A:AURKB|AURKB]]|-1.96|0.63| |[[:human_genes:G:GPR126|GPR126]]|-1.96|0.52| |[[:human_genes:Z:ZNF488|ZNF488]]|-1.95|0.62| |[[:human_genes:R:REEP2|REEP2]]|-1.95|0.52| |[[:human_genes:T:TRIP6|TRIP6]]|-1.95|0.52| |[[:human_genes:A:ANKH|ANKH]]|2.12|0.23| |[[:human_genes:C:C2orf27B|C2orf27B]]|2.13|0.33| |[[:human_genes:L:LRR1|LRR1]]|2.13|0.34| |[[:human_genes:P:POLR2A|POLR2A]]|2.19|0.37| |[[:human_genes:W:WEE1|WEE1]]|2.19|0.37| |[[:human_genes:R:RPS5|RPS5]]|2.19|0.27| |[[:human_genes:U:UTY|UTY]]|2.23|0.16| |[[:human_genes:L:LOC643669|LOC643669]]|2.25|0.23| |[[:human_genes:P:PTGFR|PTGFR]]|2.26|0.16| |[[:human_genes:E:EIF2B4|EIF2B4]]|2.30|0.33| |[[:human_genes:R:RPL14|RPL14]]|2.32|0.34| |[[:human_genes:S:SYNPR|SYNPR]]|2.35|0.12| |[[:human_genes:P:PSME3|PSME3]]|2.38|0.12| |[[:human_genes:S:SLC25A36|SLC25A36]]|2.39|0.12| |[[:human_genes:R:RPL8|RPL8]]|2.40|0.49| |[[:human_genes:M:MGAT4D|MGAT4D]]|2.45|0.47| |[[:human_genes:T:TPR|TPR]]|2.47|0.12| |[[:human_genes:A:ANKRD40|ANKRD40]]|2.51|0.16| |[[:human_genes:Z:ZNF713|ZNF713]]|2.53|0.16| |[[:human_genes:A:AGK|AGK]]|2.54|0.08| |[[:human_genes:A:ATF6|ATF6]]|2.54|0.12| |[[:human_genes:N:NGLY1|NGLY1]]|2.57|0.08| |[[:human_genes:P:PRPF8|PRPF8]]|2.59|0.34| |[[:human_genes:A:ANKRD36|ANKRD36]]|2.60|0.08| |[[:human_genes:C:COPE|COPE]]|2.68|0.27| |[[:human_genes:R:RPS12|RPS12]]|2.75|0.08| |[[:human_genes:S:SCAF1|SCAF1]]|2.82|0.36| |[[:human_genes:C:CCT4|CCT4]]|2.96|0.34| |[[:human_genes:R:RBM39|RBM39]]|3.09|0.33| |[[:human_genes:S:SUPT6H|SUPT6H]]|3.25|0.16| </modal> <modal id="modal_screen_similarity" size="lg" title="Correlated Screens"> ^Screen^Correlation^Plot^ |[[:results:exp23|Nocodazole 0.02μM R00 exp23]]|0.07|<button type="primary" size="small" modal="1">View</button>| |[[:results:exp31|Rifampicin 1μM R00 exp31]]|0.068|<button type="primary" size="small" modal="2">View</button>| |[[:results:exp209|Deguelin 0.15μM R05 exp209]]|0.054|<button type="primary" size="small" modal="3">View</button>| |[[:results:exp295|Pyronaridine 1μM R06 exp295]]|0.054|<button type="primary" size="small" modal="4">View</button>| |[[:results:exp10|CCCP 0.1μM R00 exp10]]|0.081|<button type="primary" size="small" modal="5">View</button>| |[[:results:exp11|CCCP 1μM R00 exp11]]|0.075|<button type="primary" size="small" modal="6">View</button>| </modal> <modal id="1" size="lg" title="Chloramphenicol 2μM R00 exp12 vs Nocodazole 0.02μM R00 exp23"> {{:chemogenomics:comparison_plots:exp12_vs_exp23.png?nolink |}} </modal> <modal id="2" size="lg" title="Chloramphenicol 2μM R00 exp12 vs Rifampicin 1μM R00 exp31"> {{:chemogenomics:comparison_plots:exp12_vs_exp31.png?nolink |}} </modal> <modal id="3" size="lg" title="Chloramphenicol 2μM R00 exp12 vs Deguelin 0.15μM R05 exp209"> {{:chemogenomics:comparison_plots:exp12_vs_exp209.png?nolink |}} </modal> <modal id="4" size="lg" title="Chloramphenicol 2μM R00 exp12 vs Pyronaridine 1μM R06 exp295"> {{:chemogenomics:comparison_plots:exp12_vs_exp295.png?nolink |}} </modal> <modal id="5" size="lg" title="CCCP 0.1μM R00 exp10 vs Chloramphenicol 2μM R00 exp12"> {{:chemogenomics:comparison_plots:exp10_vs_exp12.png?nolink |}} </modal> <modal id="6" size="lg" title="CCCP 1μM R00 exp11 vs Chloramphenicol 2μM R00 exp12"> {{:chemogenomics:comparison_plots:exp11_vs_exp12.png?nolink |}} </modal> <modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms"> ^GO Term^Fold Change^Genes^ |CST complex|637.73|TEN1,CTC1| </modal> <modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal> Last modified: 2026/01/07 22:37by 127.0.0.1