Oligomycin-A 20μM R00 exp26 [The Human Chemical-Genetic Interaction Map Project]

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====== Oligomycin-A 20μM R00 exp26 ======
==== Mechanism of Action ====
Inhibits F0 proton channel of mitochondrial ATP synthase, blocks electron flow through ETC
  * **<color #00a2e8>Class / Subclass 1:</color>** Organelle Function / Mitochondrial Inhibitor




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Oligomycin A%%
    * **<color #00a2e8>Synonyms:</color>** MCH 32
    * **<color #00a2e8>CAS #:</color>** 579-13-5
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5281899|5281899]]
    * **<color #00a2e8>IUPAC:</color>** %%(1S,4E,5'R,6R,6'R,7S,8R,10S,11S,12R,14S,15R,16S,18E,20E,22S,25R,27S,28R,29S)-22-ethyl-7,11,14,15-tetrahydroxy-6'-[(2S)-2-hydroxypropyl]-5',6,8,10,12,14,16,28,29-nonamethylspiro[2,26-dioxabicyclo[23.3.1]nonacosa-4,18,20-triene-27,2'-oxane]-3,9,13-trione%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C45H74O11/c1-12-34-17-15-13-14-16-27(4)42(51)44(11,53)43(52)32(9)40(50)31(8)39(49)30(7)38(48)26(3)18-21-37(47)54-41-29(6)35(20-19-34)55-45(33(41)10)23-22-25(2)36(56-45)24-28(5)46/h13-15,17-18,21,25-36,38,40-42,46,48,50-51,53H,12,16,19-20,22-24H2,1-11H3/b14-13+,17-15+,21-18+/t25-,26-,27+,28+,29+,30-,31-,32-,33-,34-,35-,36-,38+,40+,41+,42-,44+,45-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** MNULEGDCPYONBU-WMBHJXFZSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 791.1
    * **<color #00a2e8>Canonical SMILES:</color>** CCC1CCC2C(C(C(C3(O2)CCC(C(O3)CC(C)O)C)C)OC(=O)C=CC(C(C(C(=O)C(C(C(C(=O)C(C(C(CC=CC=C1)C)O)(C)O)C)O)C)C)O)C)C
    * **<color #00a2e8>Isomeric SMILES:</color>** CC[C@H]\\1CC[C@@H]2[C@@H]([C@@H]([C@H]([C@@]3(O2)CC[C@H]([C@H](O3)C[C@H](C)O)C)C)OC(=O)/C=C/[C@H]([C@@H]([C@H](C(=O)[C@H]([C@@H]([C@H](C(=O)[C@@]([C@@H]([C@H](C/C=C/C=C1)C)O)(C)O)C)O)C)C)O)C)C
    * **<color #00a2e8>Molecular Formula:</color>** C45H74O11
{{:chemogenomics:structures:chem-0014.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Tocris Bioscience
    * **<color #00a2e8>Catalog #:</color>** 4110
    * **<color #00a2e8>Lot #:</color>** 6A/200926
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C45H74O11 791.53039; found 791.53025
</panel>


<panel title='Dose Response Curve'>
Dose response curve not available.
</panel>


</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 00
    * **<color #00a2e8>Dose</color>**: 20µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 0.3
    * **<color #00a2e8>Numbers of reads</color>**: 9869667


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|3/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Oligomycin_A_20uM_Round-0_exp26.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Oligomycin-A 20μM R00 exp26'>
{{:chemogenomics:cranks_plots:exp26.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:R:RAB3GAP2|RAB3GAP2]]|-4.27|<0.01|
|[[:human_genes:A:ATP6AP1|ATP6AP1]]|-3.24|0.22|
|[[:human_genes:U:UBE2Q2L|UBE2Q2L]]|-3.02|0.20|
|[[:human_genes:T:TBC1D20|TBC1D20]]|-2.90|0.04|
|[[:human_genes:R:RAB18|RAB18]]|-2.83|0.02|
|[[:human_genes:D:DONSON|DONSON]]|-2.77|0.23|
|[[:human_genes:O:OSBP|OSBP]]|-2.75|0.20|
|[[:human_genes:O:OR10G3|OR10G3]]|-2.71|0.08|
|[[:human_genes:P:POLA1|POLA1]]|-2.67|0.40|
|[[:human_genes:P:PSMA3|PSMA3]]|-2.65|0.21|
|[[:human_genes:F:FLVCR1|FLVCR1]]|-2.60|0.07|
|[[:human_genes:B:BNIP1|BNIP1]]|-2.59|0.06|
|[[:human_genes:C:COX7A2L|COX7A2L]]|-2.54|0.06|
|[[:human_genes:P:PCYT1A|PCYT1A]]|-2.51|0.06|
|[[:human_genes:A:ACOT8|ACOT8]]|-2.49|0.06|
|[[:human_genes:A:ATP6V0A2|ATP6V0A2]]|-2.39|0.13|
|[[:human_genes:P:POLA2|POLA2]]|-2.36|0.31|
|[[:human_genes:P:PI4KB|PI4KB]]|-2.35|0.09|
|[[:human_genes:L:LRP3|LRP3]]|-2.35|0.09|
|[[:human_genes:A:ATP6V0B|ATP6V0B]]|-2.33|0.20|
|[[:human_genes:P:PCDHGC5|PCDHGC5]]|-2.31|0.15|
|[[:human_genes:A:AGPS|AGPS]]|-2.31|0.15|
|[[:human_genes:H:HSD17B4|HSD17B4]]|-2.29|0.11|
|[[:human_genes:C:COPE|COPE]]|-2.29|0.34|
|[[:human_genes:G:GOLGA6L1|GOLGA6L1]]|-2.29|0.40|
|[[:human_genes:R:RNF103-CHMP3|RNF103-CHMP3]]|-2.25|0.17|
|[[:human_genes:P:PSAP|PSAP]]|-2.25|0.17|
|[[:human_genes:T:TMEM138|TMEM138]]|-2.23|0.14|
|[[:human_genes:C:COPA|COPA]]|-2.23|0.36|
|[[:human_genes:F:FAM231C|FAM231C]]|-2.23|0.21|
|[[:human_genes:N:NAA25|NAA25]]|1.95|0.66|
|[[:human_genes:M:MRPL47|MRPL47]]|1.95|0.42|
|[[:human_genes:C:CCNC|CCNC]]|1.97|0.39|
|[[:human_genes:G:GABARAP|GABARAP]]|1.97|0.39|
|[[:human_genes:M:MMP9|MMP9]]|1.97|0.44|
|[[:human_genes:P:POLR3H|POLR3H]]|1.98|0.39|
|[[:human_genes:C:C9orf92|C9orf92]]|2.02|0.35|
|[[:human_genes:S:ST20|ST20]]|2.03|0.76|
|[[:human_genes:R:RABGGTB|RABGGTB]]|2.05|0.33|
|[[:human_genes:E:EIF3F|EIF3F]]|2.06|0.33|
|[[:human_genes:T:TRAPPC9|TRAPPC9]]|2.06|0.33|
|[[:human_genes:M:MTRNR2L10|MTRNR2L10]]|2.06|0.45|
|[[:human_genes:E:EIF3I|EIF3I]]|2.06|0.45|
|[[:human_genes:D:DKC1|DKC1]]|2.07|0.66|
|[[:human_genes:C:CRCP|CRCP]]|2.08|0.33|
|[[:human_genes:G:GFER|GFER]]|2.10|0.44|
|[[:human_genes:C:CAB39|CAB39]]|2.11|0.33|
|[[:human_genes:A:ANKRD30A|ANKRD30A]]|2.11|0.33|
|[[:human_genes:W:WDR48|WDR48]]|2.18|0.33|
|[[:human_genes:S:SEC31A|SEC31A]]|2.21|0.23|
|[[:human_genes:H:HHLA3|HHLA3]]|2.24|0.22|
|[[:human_genes:T:TXNDC17|TXNDC17]]|2.27|0.22|
|[[:human_genes:T:TXNRD1|TXNRD1]]|2.30|0.34|
|[[:human_genes:S:SMIM15|SMIM15]]|2.31|0.22|
|[[:human_genes:K:KARS|KARS]]|2.42|0.22|
|[[:human_genes:H:HLTF|HLTF]]|2.44|0.18|
|[[:human_genes:A:ALG5|ALG5]]|2.45|0.18|
|[[:human_genes:P:PRMT5|PRMT5]]|2.46|0.56|
|[[:human_genes:U:UTP14C|UTP14C]]|2.52|0.18|
|[[:human_genes:N:NARS|NARS]]|2.56|0.22|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp34|Rotenone 20μM R00 exp34]]|0.072|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp28|Pimelic-diphenylamide-106 5μM R00 exp28]]|0.062|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp17|DABN 20μM R00 exp17]]|0.092|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp7|Bortezomib 0.05μM R00 exp7]]|0.072|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp19|Etoposide 1μM R00 exp19]]|0.062|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp8|Brefeldin A 0.02μM R00 exp8]]|0.054|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp199|Etoposide 0.3μM R05 exp199]]|0.052|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp469|CFI-400945 25μM R08 exp469]]|0.052|<button type="primary" size="small" modal="8">View</button>|
</modal>


<modal id="1" size="lg" title="Oligomycin-A 20μM R00 exp26  vs  Rotenone 20μM R00 exp34">
{{:chemogenomics:comparison_plots:exp26_vs_exp34.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Oligomycin-A 20μM R00 exp26  vs  Pimelic-diphenylamide-106 5μM R00 exp28">
{{:chemogenomics:comparison_plots:exp26_vs_exp28.png?nolink |}}
</modal>

<modal id="3" size="lg" title="DABN 20μM R00 exp17  vs  Oligomycin-A 20μM R00 exp26">
{{:chemogenomics:comparison_plots:exp17_vs_exp26.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Bortezomib 0.05μM R00 exp7  vs  Oligomycin-A 20μM R00 exp26">
{{:chemogenomics:comparison_plots:exp26_vs_exp7.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Etoposide 1μM R00 exp19  vs  Oligomycin-A 20μM R00 exp26">
{{:chemogenomics:comparison_plots:exp19_vs_exp26.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Brefeldin A 0.02μM R00 exp8  vs  Oligomycin-A 20μM R00 exp26">
{{:chemogenomics:comparison_plots:exp26_vs_exp8.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Etoposide 0.3μM R05 exp199  vs  Oligomycin-A 20μM R00 exp26">
{{:chemogenomics:comparison_plots:exp199_vs_exp26.png?nolink |}}
</modal>

<modal id="8" size="lg" title="CFI-400945 25μM R08 exp469  vs  Oligomycin-A 20μM R00 exp26">
{{:chemogenomics:comparison_plots:exp26_vs_exp469.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>