Cisplatin 0.35μM R06 exp273 [The Human Chemical-Genetic Interaction Map Project]

This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong.
====== Cisplatin 0.35μM R06 exp273 ======
==== Mechanism of Action ====
Intrastrand cross-linker
  * **<color #00a2e8>Class / Subclass 1:</color>** DNA Damage, Repair and Replication / Inter/Intra-strand Crosslinker




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Cisplatin%%
    * **<color #00a2e8>Synonyms:</color>** CDDP; cis-Diaminodichloroplatinum
    * **<color #00a2e8>CAS #:</color>** 15663-27-1
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/5702198|5702198]]
    * **<color #00a2e8>IUPAC:</color>** %%azane;dichloroplatinum%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/2ClH.2H3N.Pt/h2*1H;2*1H3;/q;;;;+2/p-2
    * **<color #00a2e8>INCHI Key:</color>** LXZZYRPGZAFOLE-UHFFFAOYSA-L
    * **<color #00a2e8>Molecular Weight:</color>** 300
    * **<color #00a2e8>Canonical SMILES:</color>** N.N.Cl[Pt]Cl
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** Cl2H6N2Pt
{{:chemogenomics:structures:chem-0165.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Sigma-Aldrich
    * **<color #00a2e8>Catalog #:</color>** P4394
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>XRPD:</color>** Expected (NH3)2PtCl2; found (NH3)2PtCl2
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Cisplatin
    * **<color #00a2e8>Min:</color>** 4.5895; **<color #00a2e8>Max:</color>** 99.6466
{{:chemogenomics:dose_response:dr_317.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |0.2153    |
| IC40  |N/A    |
| IC50  |0.4700    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 06
    * **<color #00a2e8>Dose</color>**: 350nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 7.0
    * **<color #00a2e8>Numbers of reads</color>**: 7163875


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|2/1|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Cisplatin_0.35uM_Round-6_exp273.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Cisplatin 0.35μM R06 exp273'>
{{:chemogenomics:cranks_plots:exp273.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:F:FANCC|FANCC]]|-3.26|<0.01|
|[[:human_genes:M:MAD2L2|MAD2L2]]|-3.19|<0.01|
|[[:human_genes:P:PYGO1|PYGO1]]|-2.37|0.28|
|[[:human_genes:R:RBM14|RBM14]]|-2.34|0.63|
|[[:human_genes:R:RBM14-RBM4|RBM14-RBM4]]|-2.30|0.63|
|[[:human_genes:A:ARFRP1|ARFRP1]]|-2.28|0.63|
|[[:human_genes:C:CD86|CD86]]|-2.27|0.63|
|[[:human_genes:T:TMC2|TMC2]]|-2.26|0.63|
|[[:human_genes:T:TRIM42|TRIM42]]|-2.20|0.49|
|[[:human_genes:C:COL6A3|COL6A3]]|-2.18|0.49|
|[[:human_genes:P:PTPLAD1|PTPLAD1]]|-2.12|0.58|
|[[:human_genes:E:EXOC8|EXOC8]]|-2.12|0.63|
|[[:human_genes:R:RPS15A|RPS15A]]|-2.10|0.90|
|[[:human_genes:D:DEFB128|DEFB128]]|-2.06|0.68|
|[[:human_genes:C:COX5A|COX5A]]|-2.06|0.63|
|[[:human_genes:N:NUDCD3|NUDCD3]]|-2.03|0.63|
|[[:human_genes:M:MUC6|MUC6]]|-2.02|0.63|
|[[:human_genes:M:MRPL53|MRPL53]]|-2.02|0.63|
|[[:human_genes:C:CNBD2|CNBD2]]|-2.01|0.63|
|[[:human_genes:G:GMPPB|GMPPB]]|-2.01|0.83|
|[[:human_genes:F:FANCF|FANCF]]|-1.99|0.63|
|[[:human_genes:S:SEC62|SEC62]]|-1.97|0.63|
|[[:human_genes:C:COA5|COA5]]|-1.96|0.85|
|[[:human_genes:A:AGPAT2|AGPAT2]]|-1.96|0.63|
|[[:human_genes:M:MRPS25|MRPS25]]|-1.95|0.63|
|[[:human_genes:P:PSMD4|PSMD4]]|-1.94|0.81|
|[[:human_genes:C:CEPT1|CEPT1]]|-1.94|0.63|
|[[:human_genes:R:RXFP2|RXFP2]]|-1.93|0.63|
|[[:human_genes:A:ASAP1|ASAP1]]|-1.92|0.63|
|[[:human_genes:D:DPAGT1|DPAGT1]]|-1.90|0.63|
|[[:human_genes:P:PTPLAD2|PTPLAD2]]|1.88|0.83|
|[[:human_genes:P:PRPF31|PRPF31]]|1.91|0.90|
|[[:human_genes:T:THBS4|THBS4]]|1.91|0.83|
|[[:human_genes:S:SPTB|SPTB]]|1.92|0.83|
|[[:human_genes:K:KPNB1|KPNB1]]|1.93|0.83|
|[[:human_genes:U:UTP18|UTP18]]|1.94|0.72|
|[[:human_genes:R:RPA1|RPA1]]|1.94|0.90|
|[[:human_genes:T:TRIM54|TRIM54]]|1.97|0.66|
|[[:human_genes:K:KIF24|KIF24]]|1.99|0.66|
|[[:human_genes:D:DNAJB4|DNAJB4]]|1.99|0.66|
|[[:human_genes:C:COL6A5|COL6A5]]|2.00|0.66|
|[[:human_genes:A:ANKRD13C|ANKRD13C]]|2.01|0.83|
|[[:human_genes:S:SEC24B|SEC24B]]|2.01|0.66|
|[[:human_genes:G:GCNT2|GCNT2]]|2.02|0.66|
|[[:human_genes:P:PSMB4|PSMB4]]|2.03|0.83|
|[[:human_genes:I:IRX6|IRX6]]|2.03|0.83|
|[[:human_genes:A:AP3S1|AP3S1]]|2.03|0.66|
|[[:human_genes:G:GLUL|GLUL]]|2.04|0.66|
|[[:human_genes:C:CELF5|CELF5]]|2.04|0.66|
|[[:human_genes:C:C3orf56|C3orf56]]|2.05|0.66|
|[[:human_genes:S:SNRPF|SNRPF]]|2.12|0.90|
|[[:human_genes:N:NXF1|NXF1]]|2.33|0.75|
|[[:human_genes:T:TUBB4A|TUBB4A]]|2.40|0.66|
|[[:human_genes:S:SUGP1|SUGP1]]|2.44|0.66|
|[[:human_genes:T:TBC1D3I|TBC1D3I]]|2.69|0.83|
|[[:human_genes:T:TBC1D3E|TBC1D3E]]|2.69|0.83|
|[[:human_genes:T:TBC1D3K|TBC1D3K]]|2.70|0.83|
|[[:human_genes:T:TBC1D3L|TBC1D3L]]|2.70|0.83|
|[[:human_genes:U:USP17L17|USP17L17]]|2.74|0.83|
|[[:human_genes:P:PDE4B|PDE4B]]|2.85|0.05|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp286|HMS-I2 1μM R06 exp286]]|0.056|<button type="primary" size="small" modal="1">View</button>|
</modal>


<modal id="1" size="lg" title="Cisplatin 0.35μM R06 exp273  vs  HMS-I2 1μM R06 exp286">
{{:chemogenomics:comparison_plots:exp273_vs_exp286.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>