FK-506 30μM R07 exp359 [The Human Chemical-Genetic Interaction Map Project]

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====== FK-506 30μM R07 exp359 ======
==== Mechanism of Action ====
Inhibits calcineurin
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Phosphatase Inhibitor




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Tacrolimus%%
    * **<color #00a2e8>Synonyms:</color>** FK506; Fujimycin; FR900506
    * **<color #00a2e8>CAS #:</color>** 104987-11-3
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/445643|445643]]
    * **<color #00a2e8>IUPAC:</color>** %%(1R,9S,12S,13R,14S,17R,18E,21S,23S,24R,25S,27R)-1,14-dihydroxy-12-[(E)-1-[(1R,3R,4R)-4-hydroxy-3-methoxycyclohexyl]prop-1-en-2-yl]-23,25-dimethoxy-13,19,21,27-tetramethyl-17-prop-2-enyl-11,28-dioxa-4-azatricyclo[22.3.1.04,9]octacos-18-ene-2,3,10,16-tetrone%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C44H69NO12/c1-10-13-31-19-25(2)18-26(3)20-37(54-8)40-38(55-9)22-28(5)44(52,57-40)41(49)42(50)45-17-12-11-14-32(45)43(51)56-39(29(6)34(47)24-35(31)48)27(4)21-30-15-16-33(46)36(23-30)53-7/h10,19,21,26,28-34,36-40,46-47,52H,1,11-18,20,22-24H2,2-9H3/b25-19+,27-21+/t26-,28+,29+,30-,31+,32-,33+,34-,36+,37-,38-,39+,40+,44+/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** QJJXYPPXXYFBGM-LFZNUXCKSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 804
    * **<color #00a2e8>Canonical SMILES:</color>** CC1CC(C2C(CC(C(O2)(C(=O)C(=O)N3CCCCC3C(=O)OC(C(C(CC(=O)C(C=C(C1)C)CC=C)O)C)C(=CC4CCC(C(C4)OC)O)C)O)C)OC)OC
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@@H]1C[C@@H]([C@@H]2[C@H](C[C@H]([C@@](O2)(C(=O)C(=O)N3CCCC[C@H]3C(=O)O[C@@H]([C@@H]([C@H](CC(=O)[C@@H](/C=C(/C1)\\C)CC=C)O)C)/C(=C/[C@@H]4CC[C@H]([C@@H](C4)OC)O)/C)O)C)OC)OC
    * **<color #00a2e8>Molecular Formula:</color>** C44H69NO12
{{:chemogenomics:structures:chem-0213.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Toronto Research Chemicals
    * **<color #00a2e8>Catalog #:</color>** F370000
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+Na)+ Calcd for C44H69NO12 826.4712; found 826.47365
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** FK-506
    * **<color #00a2e8>Min:</color>** -17.1824; **<color #00a2e8>Max:</color>** 93.4566
{{:chemogenomics:dose_response:dr_361.png?nolink&500 |}}
\\ 
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |29.9866    |
| IC30  |31.6841    |
| IC40  |33.1471    |
| IC50  |34.5490    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 30µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 4.2
    * **<color #00a2e8>Numbers of reads</color>**: 20870386


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|21/8|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/FK-506_30uM_Round-7_exp359.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='FK-506 30μM R07 exp359'>
{{:chemogenomics:cranks_plots:exp359.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:F:FLVCR1|FLVCR1]]|-4.02|<0.01|
|[[:human_genes:S:SLC5A3|SLC5A3]]|-3.81|<0.01|
|[[:human_genes:C:CEPT1|CEPT1]]|-3.61|<0.01|
|[[:human_genes:C:CHKA|CHKA]]|-3.41|<0.01|
|[[:human_genes:Y:YPEL5|YPEL5]]|-3.36|<0.01|
|[[:human_genes:M:MAEA|MAEA]]|-3.32|<0.01|
|[[:human_genes:T:TCF4|TCF4]]|-3.20|<0.01|
|[[:human_genes:C:CHMP7|CHMP7]]|-3.19|<0.01|
|[[:human_genes:U:UBE2H|UBE2H]]|-3.17|<0.01|
|[[:human_genes:W:WDR26|WDR26]]|-3.01|0.01|
|[[:human_genes:D:DERL2|DERL2]]|-2.81|<0.01|
|[[:human_genes:D:DERL1|DERL1]]|-2.71|<0.01|
|[[:human_genes:I:IMMT|IMMT]]|-2.67|0.02|
|[[:human_genes:U:UMPS|UMPS]]|-2.64|0.29|
|[[:human_genes:U:UXS1|UXS1]]|-2.64|0.01|
|[[:human_genes:F:FAM194B|FAM194B]]|-2.63|0.03|
|[[:human_genes:M:MSI2|MSI2]]|-2.62|0.03|
|[[:human_genes:G:GID8|GID8]]|-2.56|0.02|
|[[:human_genes:C:CSK|CSK]]|-2.53|0.02|
|[[:human_genes:B:BRPF3|BRPF3]]|-2.39|0.13|
|[[:human_genes:S:SSU72|SSU72]]|-2.38|0.04|
|[[:human_genes:P:PCYT1A|PCYT1A]]|-2.34|0.05|
|[[:human_genes:M:MTX2|MTX2]]|-2.33|0.05|
|[[:human_genes:Q:QRICH1|QRICH1]]|-2.31|0.16|
|[[:human_genes:T:TNFAIP3|TNFAIP3]]|-2.27|0.07|
|[[:human_genes:O:OPA3|OPA3]]|-2.25|0.07|
|[[:human_genes:W:WIPI2|WIPI2]]|-2.25|0.07|
|[[:human_genes:K:KRTAP10-4|KRTAP10-4]]|-2.24|0.27|
|[[:human_genes:T:TRAF2|TRAF2]]|-2.24|0.13|
|[[:human_genes:S:SPG7|SPG7]]|-2.20|0.14|
|[[:human_genes:P:PRMT3|PRMT3]]|2.09|0.21|
|[[:human_genes:B:BHLHB9|BHLHB9]]|2.11|0.25|
|[[:human_genes:C:COPE|COPE]]|2.11|0.61|
|[[:human_genes:P:PREPL|PREPL]]|2.11|0.25|
|[[:human_genes:K:KCTD5|KCTD5]]|2.17|0.17|
|[[:human_genes:P:POLR2J2|POLR2J2]]|2.19|0.51|
|[[:human_genes:M:MIPEP|MIPEP]]|2.22|0.14|
|[[:human_genes:A:AKAP12|AKAP12]]|2.23|0.20|
|[[:human_genes:M:MNS1|MNS1]]|2.24|0.26|
|[[:human_genes:E:ELP3|ELP3]]|2.25|0.13|
|[[:human_genes:A:ALDH18A1|ALDH18A1]]|2.26|0.18|
|[[:human_genes:Z:ZNF461|ZNF461]]|2.27|0.18|
|[[:human_genes:R:RPS9|RPS9]]|2.27|0.50|
|[[:human_genes:L:LARS2|LARS2]]|2.27|0.18|
|[[:human_genes:L:LRRC63|LRRC63]]|2.28|0.54|
|[[:human_genes:D:DAZAP1|DAZAP1]]|2.29|0.11|
|[[:human_genes:T:TXNIP|TXNIP]]|2.29|0.11|
|[[:human_genes:B:BCL2L15|BCL2L15]]|2.34|0.10|
|[[:human_genes:K:KPNB1|KPNB1]]|2.36|0.47|
|[[:human_genes:K:KMT2B|KMT2B]]|2.38|0.13|
|[[:human_genes:S:SCAP|SCAP]]|2.42|0.07|
|[[:human_genes:H:HNRNPU|HNRNPU]]|2.49|0.05|
|[[:human_genes:D:DPH1|DPH1]]|2.68|0.02|
|[[:human_genes:P:PMVK|PMVK]]|2.68|0.02|
|[[:human_genes:D:DPH3|DPH3]]|2.71|0.02|
|[[:human_genes:P:PPA1|PPA1]]|2.86|0.05|
|[[:human_genes:L:LIG1|LIG1]]|2.86|0.38|
|[[:human_genes:D:DNAJC24|DNAJC24]]|2.98|<0.01|
|[[:human_genes:E:EIF4A1|EIF4A1]]|2.99|0.02|
|[[:human_genes:S:STARD7|STARD7]]|6.06|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp78|Pterostilbene 16μM R02 exp78]]|0.078|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp442|Ibrutinib 10μM R08 exp442]]|0.077|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp90|WYE-354 6μM R02 exp90]]|0.068|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp492|iCRT14 30μM R08 exp492]]|0.063|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp502|Milciclib 2μM R08 exp502]]|0.06|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp530|Thioridazine 5μM R08 exp530]]|0.059|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp85|UM0129480 7μM R02 exp85]]|0.053|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp497|Lead acetate 2000μM R08 exp497]]|0.052|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp498|Lead acetate 2000μM R08 exp498 no dilution day6]]|0.052|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp75|MK-1775 0.32μM R02 exp75]]|0.052|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp227|Cryptotanshinone 12μM R05 exp227]]|0.072|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp269|Bisphenol A 100μM R06 exp269]]|0.068|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp445|∆-9-Tetrahydrocannabinol 30μM R08 exp445]]|0.067|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp453|B02 10μM R08 exp453]]|0.065|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|0.058|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp224|CB-839 10μM R05 exp224]]|0.058|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp447|Amiloride 100μM R08 exp447]]|0.056|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|0.053|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp344|Chlorpromazine 10μM R07 exp344]]|0.051|<button type="primary" size="small" modal="19">View</button>|
|[[:results:exp214|2-Deoxy-D-glucose 800μM R05 exp214]]|0.051|<button type="primary" size="small" modal="20">View</button>|
</modal>


<modal id="1" size="lg" title="FK-506 30μM R07 exp359  vs  Pterostilbene 16μM R02 exp78">
{{:chemogenomics:comparison_plots:exp359_vs_exp78.png?nolink |}}
</modal>

<modal id="2" size="lg" title="FK-506 30μM R07 exp359  vs  Ibrutinib 10μM R08 exp442">
{{:chemogenomics:comparison_plots:exp359_vs_exp442.png?nolink |}}
</modal>

<modal id="3" size="lg" title="FK-506 30μM R07 exp359  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp359_vs_exp90.png?nolink |}}
</modal>

<modal id="4" size="lg" title="FK-506 30μM R07 exp359  vs  iCRT14 30μM R08 exp492">
{{:chemogenomics:comparison_plots:exp359_vs_exp492.png?nolink |}}
</modal>

<modal id="5" size="lg" title="FK-506 30μM R07 exp359  vs  Milciclib 2μM R08 exp502">
{{:chemogenomics:comparison_plots:exp359_vs_exp502.png?nolink |}}
</modal>

<modal id="6" size="lg" title="FK-506 30μM R07 exp359  vs  Thioridazine 5μM R08 exp530">
{{:chemogenomics:comparison_plots:exp359_vs_exp530.png?nolink |}}
</modal>

<modal id="7" size="lg" title="FK-506 30μM R07 exp359  vs  UM0129480 7μM R02 exp85">
{{:chemogenomics:comparison_plots:exp359_vs_exp85.png?nolink |}}
</modal>

<modal id="8" size="lg" title="FK-506 30μM R07 exp359  vs  Lead acetate 2000μM R08 exp497">
{{:chemogenomics:comparison_plots:exp359_vs_exp497.png?nolink |}}
</modal>

<modal id="9" size="lg" title="FK-506 30μM R07 exp359  vs  Lead acetate 2000μM R08 exp498 no dilution day6">
{{:chemogenomics:comparison_plots:exp359_vs_exp498.png?nolink |}}
</modal>

<modal id="10" size="lg" title="FK-506 30μM R07 exp359  vs  MK-1775 0.32μM R02 exp75">
{{:chemogenomics:comparison_plots:exp359_vs_exp75.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Cryptotanshinone 12μM R05 exp227  vs  FK-506 30μM R07 exp359">
{{:chemogenomics:comparison_plots:exp227_vs_exp359.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Bisphenol A 100μM R06 exp269  vs  FK-506 30μM R07 exp359">
{{:chemogenomics:comparison_plots:exp269_vs_exp359.png?nolink |}}
</modal>

<modal id="13" size="lg" title="∆-9-Tetrahydrocannabinol 30μM R08 exp445  vs  FK-506 30μM R07 exp359">
{{:chemogenomics:comparison_plots:exp359_vs_exp445.png?nolink |}}
</modal>

<modal id="14" size="lg" title="B02 10μM R08 exp453  vs  FK-506 30μM R07 exp359">
{{:chemogenomics:comparison_plots:exp359_vs_exp453.png?nolink |}}
</modal>

<modal id="15" size="lg" title="2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312  vs  FK-506 30μM R07 exp359">
{{:chemogenomics:comparison_plots:exp312_vs_exp359.png?nolink |}}
</modal>

<modal id="16" size="lg" title="CB-839 10μM R05 exp224  vs  FK-506 30μM R07 exp359">
{{:chemogenomics:comparison_plots:exp224_vs_exp359.png?nolink |}}
</modal>

<modal id="17" size="lg" title="Amiloride 100μM R08 exp447  vs  FK-506 30μM R07 exp359">
{{:chemogenomics:comparison_plots:exp359_vs_exp447.png?nolink |}}
</modal>

<modal id="18" size="lg" title="BVD-523 15μM R02 exp67  vs  FK-506 30μM R07 exp359">
{{:chemogenomics:comparison_plots:exp359_vs_exp67.png?nolink |}}
</modal>

<modal id="19" size="lg" title="Chlorpromazine 10μM R07 exp344  vs  FK-506 30μM R07 exp359">
{{:chemogenomics:comparison_plots:exp344_vs_exp359.png?nolink |}}
</modal>

<modal id="20" size="lg" title="2-Deoxy-D-glucose 800μM R05 exp214  vs  FK-506 30μM R07 exp359">
{{:chemogenomics:comparison_plots:exp214_vs_exp359.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|CDP-choline pathway|296.05|CEPT1,CHKA,PCYT1A|
|GID complex|296.05|MAEA,WDR26|
|Hrd1p ubiquitin ligase ERAD-L complex|236.84|DERL2,DERL1|
|phosphatidylcholine biosynthetic process|74.01|CEPT1,CHKA,PCYT1A|
|ubiquitin ligase complex|13.33|YPEL5,MAEA,WDR26,DERL2,DERL1,GID8,TRAF2|
|proteasome-mediated ubiquitin-dependent protein catabolic process|10.30|MAEA,UBE2H,WDR26,DERL2,DERL1,GID8|
|proteasomal protein catabolic process|9.35|MAEA,UBE2H,WDR26,DERL2,DERL1,GID8|
|ubiquitin-dependent protein catabolic process|9.27|MAEA,CHMP7,UBE2H,WDR26,DERL2,DERL1,GID8,TNFAIP3|
|modification-dependent protein catabolic process|9.09|MAEA,CHMP7,UBE2H,WDR26,DERL2,DERL1,GID8,TNFAIP3|
|modification-dependent macromolecule catabolic process|8.90|MAEA,CHMP7,UBE2H,WDR26,DERL2,DERL1,GID8,TNFAIP3|
|proteolysis involved in protein catabolic process|8.12|MAEA,CHMP7,UBE2H,WDR26,DERL2,DERL1,GID8,TNFAIP3|
|protein catabolic process|7.67|MAEA,CHMP7,UBE2H,WDR26,DERL2,DERL1,GID8,TNFAIP3,TRAF2|
|macromolecule catabolic process|5.78|MAEA,CHMP7,UBE2H,WDR26,DERL2,DERL1,GID8,TNFAIP3,TRAF2|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>