WYE-354 6μM R02 exp90 [The Human Chemical-Genetic Interaction Map Project]

This page is read only. You can view the source, but not change it. Ask your administrator if you think this is wrong.
====== WYE-354 6μM R02 exp90 ======
==== Mechanism of Action ====
Inhibits mTORC1 and mTORC2, ATP-competitive inhibitor
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Kinase Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Proteostasis / mTOR inhibitor



==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Protein References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%CID 44219749%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 1062169-56-5
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/44219749|44219749]]
    * **<color #00a2e8>IUPAC:</color>** %%methyl 4-[6-[4-(methoxycarbonylamino)phenyl]-4-morpholin-4-ylpyrazolo[3,4-d]pyrimidin-1-yl]piperidine-1-carboxylate%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C24H29N7O5/c1-34-23(32)26-17-5-3-16(4-6-17)20-27-21(29-11-13-36-14-12-29)19-15-25-31(22(19)28-20)18-7-9-30(10-8-18)24(33)35-2/h3-6,15,18H,7-14H2,1-2H3,(H,26,32)
    * **<color #00a2e8>INCHI Key:</color>** IMXHGCRIEAKIBU-UHFFFAOYSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 495.5
    * **<color #00a2e8>Canonical SMILES:</color>** COC(=O)NC1=CC=C(C=C1)C2=NC3=C(C=NN3C4CCN(CC4)C(=O)OC)C(=N2)N5CCOCC5
    * **<color #00a2e8>Isomeric SMILES:</color>** N/A
    * **<color #00a2e8>Molecular Formula:</color>** C24H29N7O5
{{:chemogenomics:structures:chem-0066.svg?nolink}}
</panel>


<panel title='Protein Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Selleck Chemicals
    * **<color #00a2e8>Catalog #:</color>** S1266
    * **<color #00a2e8>Lot #:</color>** N/A
</panel>


<panel title='Protein Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C24H29N7O5 496.23029; found 496.23044
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** WYE-354
    * **<color #00a2e8>Min:</color>** -8.8886; **<color #00a2e8>Max:</color>** 64.9280
{{:chemogenomics:dose_response:dr_30.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.3091    |
| IC30  |0.5848    |
| IC40  |0.9864    |
| IC50  |1.5936    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 02
    * **<color #00a2e8>Dose</color>**: 6µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 2.3
    * **<color #00a2e8>Numbers of reads</color>**: 8071417


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|25/3|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/WYE-354_6uM_Round-2_exp90.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='WYE-354 6μM R02 exp90'>
{{:chemogenomics:cranks_plots:exp90.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:A:ATP6V0A2|ATP6V0A2]]|-4.44|<0.01|
|[[:human_genes:S:SPTLC1|SPTLC1]]|-4.36|<0.01|
|[[:human_genes:V:VKORC1L1|VKORC1L1]]|-3.57|<0.01|
|[[:human_genes:G:GPR107|GPR107]]|-3.40|<0.01|
|[[:human_genes:S:SGMS1|SGMS1]]|-3.38|<0.01|
|[[:human_genes:C:CYB561D2|CYB561D2]]|-3.37|<0.01|
|[[:human_genes:P:PCYT1A|PCYT1A]]|-3.25|<0.01|
|[[:human_genes:S:SLC5A3|SLC5A3]]|-3.22|<0.01|
|[[:human_genes:S:SPTLC2|SPTLC2]]|-3.04|<0.01|
|[[:human_genes:O:OSBP|OSBP]]|-2.96|<0.01|
|[[:human_genes:C:CEPT1|CEPT1]]|-2.83|0.01|
|[[:human_genes:A:AEN|AEN]]|-2.80|<0.01|
|[[:human_genes:A:ARL1|ARL1]]|-2.78|<0.01|
|[[:human_genes:T:TCF4|TCF4]]|-2.71|<0.01|
|[[:human_genes:P:PI4KB|PI4KB]]|-2.71|<0.01|
|[[:human_genes:S:SOX4|SOX4]]|-2.68|0.04|
|[[:human_genes:F:FLVCR1|FLVCR1]]|-2.61|0.02|
|[[:human_genes:J:JTB|JTB]]|-2.59|0.01|
|[[:human_genes:M:MAEA|MAEA]]|-2.55|0.02|
|[[:human_genes:C:C10orf76|C10orf76]]|-2.54|0.02|
|[[:human_genes:C:C12orf23|C12orf23]]|-2.52|0.04|
|[[:human_genes:C:COPE|COPE]]|-2.52|0.52|
|[[:human_genes:W:WDR26|WDR26]]|-2.46|0.09|
|[[:human_genes:U:UXS1|UXS1]]|-2.46|0.02|
|[[:human_genes:S:SAFB|SAFB]]|-2.42|0.10|
|[[:human_genes:H:HNF1A|HNF1A]]|-2.38|0.04|
|[[:human_genes:Q:QRICH1|QRICH1]]|-2.36|0.22|
|[[:human_genes:C:CDK6|CDK6]]|-2.34|0.04|
|[[:human_genes:C:CENPM|CENPM]]|-2.33|0.38|
|[[:human_genes:S:SETD8|SETD8]]|-2.33|0.13|
|[[:human_genes:G:GPI|GPI]]|2.08|0.50|
|[[:human_genes:T:TMEM180|TMEM180]]|2.08|0.20|
|[[:human_genes:U:USP9X|USP9X]]|2.10|0.27|
|[[:human_genes:A:APCDD1|APCDD1]]|2.11|0.18|
|[[:human_genes:P:PTX3|PTX3]]|2.13|0.17|
|[[:human_genes:P:PSMB7|PSMB7]]|2.13|0.25|
|[[:human_genes:R:RANGAP1|RANGAP1]]|2.16|0.55|
|[[:human_genes:T:TRIM54|TRIM54]]|2.19|0.20|
|[[:human_genes:C:CXorf67|CXorf67]]|2.21|0.20|
|[[:human_genes:S:SRD5A3|SRD5A3]]|2.21|0.15|
|[[:human_genes:K:KTI12|KTI12]]|2.22|0.15|
|[[:human_genes:L:LEO1|LEO1]]|2.24|0.18|
|[[:human_genes:E:EIF4A1|EIF4A1]]|2.27|0.17|
|[[:human_genes:L:LLGL1|LLGL1]]|2.28|0.12|
|[[:human_genes:S:SPI1|SPI1]]|2.29|0.17|
|[[:human_genes:A:ARHGEF4|ARHGEF4]]|2.31|0.16|
|[[:human_genes:S:SLC7A1|SLC7A1]]|2.32|0.28|
|[[:human_genes:G:GRPEL1|GRPEL1]]|2.35|0.27|
|[[:human_genes:T:TMEM217|TMEM217]]|2.38|0.08|
|[[:human_genes:I:IBA57|IBA57]]|2.41|0.07|
|[[:human_genes:C:C2CD5|C2CD5]]|2.41|0.07|
|[[:human_genes:A:AMD1|AMD1]]|2.43|0.07|
|[[:human_genes:G:GNL3|GNL3]]|2.47|0.16|
|[[:human_genes:R:RPIA|RPIA]]|2.55|0.07|
|[[:human_genes:P:POLG|POLG]]|2.63|0.07|
|[[:human_genes:S:ST6GALNAC1|ST6GALNAC1]]|2.68|0.04|
|[[:human_genes:N:NUP210L|NUP210L]]|2.69|0.07|
|[[:human_genes:Z:ZNF688|ZNF688]]|2.78|0.12|
|[[:human_genes:P:PTEN|PTEN]]|2.86|0.02|
|[[:human_genes:C:CMAS|CMAS]]|3.79|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp538|ZLN024 50μM R08 exp538]]|0.061|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp38|Wortmannin 5μM R00 exp38]]|0.053|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp169|BH1 1μM R04 exp169]]|0.098|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp466|Cannabidiol 20μM R08 exp466]]|0.084|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp461|BS-181 20μM R08 exp461]]|0.082|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp224|CB-839 10μM R05 exp224]]|0.075|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp47|Lapatinib 5μM R01 exp47]]|0.071|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp469|CFI-400945 25μM R08 exp469]]|0.07|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|0.07|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp504|MK2206 4μM R08 exp504]]|0.07|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp359|FK-506 30μM R07 exp359]]|0.068|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp68|Clomiphene 4.4μM R02 exp68]]|0.067|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp445|∆-9-Tetrahydrocannabinol 30μM R08 exp445]]|0.067|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp269|Bisphenol A 100μM R06 exp269]]|0.062|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp344|Chlorpromazine 10μM R07 exp344]]|0.062|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp227|Cryptotanshinone 12μM R05 exp227]]|0.061|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp78|Pterostilbene 16μM R02 exp78]]|0.061|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp530|Thioridazine 5μM R08 exp530]]|0.06|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp507|Monensin 0.3μM R08 exp507]]|0.057|<button type="primary" size="small" modal="19">View</button>|
|[[:results:exp233|EPZ-5676 30μM R05 exp233]]|0.057|<button type="primary" size="small" modal="20">View</button>|
|[[:results:exp510|Nicotine 3000μM R08 exp510]]|0.056|<button type="primary" size="small" modal="21">View</button>|
|[[:results:exp164|Q15 1 to 2μM on day4 R04 exp164]]|0.056|<button type="primary" size="small" modal="22">View</button>|
|[[:results:exp79|Q15 2.7μM R02 exp79]]|0.054|<button type="primary" size="small" modal="23">View</button>|
|[[:results:exp326|CCT251545 20μM R07 exp326]]|0.052|<button type="primary" size="small" modal="24">View</button>|
|[[:results:exp77|Prochlorperazine 5.2μM R02 exp77]]|0.051|<button type="primary" size="small" modal="25">View</button>|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|0.051|<button type="primary" size="small" modal="26">View</button>|
</modal>


<modal id="1" size="lg" title="WYE-354 6μM R02 exp90  vs  ZLN024 50μM R08 exp538">
{{:chemogenomics:comparison_plots:exp538_vs_exp90.png?nolink |}}
</modal>

<modal id="2" size="lg" title="WYE-354 6μM R02 exp90  vs  Wortmannin 5μM R00 exp38">
{{:chemogenomics:comparison_plots:exp38_vs_exp90.png?nolink |}}
</modal>

<modal id="3" size="lg" title="BH1 1μM R04 exp169  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp169_vs_exp90.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Cannabidiol 20μM R08 exp466  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp466_vs_exp90.png?nolink |}}
</modal>

<modal id="5" size="lg" title="BS-181 20μM R08 exp461  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp461_vs_exp90.png?nolink |}}
</modal>

<modal id="6" size="lg" title="CB-839 10μM R05 exp224  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp224_vs_exp90.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Lapatinib 5μM R01 exp47  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp47_vs_exp90.png?nolink |}}
</modal>

<modal id="8" size="lg" title="CFI-400945 25μM R08 exp469  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp469_vs_exp90.png?nolink |}}
</modal>

<modal id="9" size="lg" title="BVD-523 15μM R02 exp67  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp67_vs_exp90.png?nolink |}}
</modal>

<modal id="10" size="lg" title="MK2206 4μM R08 exp504  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp504_vs_exp90.png?nolink |}}
</modal>

<modal id="11" size="lg" title="FK-506 30μM R07 exp359  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp359_vs_exp90.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Clomiphene 4.4μM R02 exp68  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp68_vs_exp90.png?nolink |}}
</modal>

<modal id="13" size="lg" title="∆-9-Tetrahydrocannabinol 30μM R08 exp445  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp445_vs_exp90.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Bisphenol A 100μM R06 exp269  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp269_vs_exp90.png?nolink |}}
</modal>

<modal id="15" size="lg" title="Chlorpromazine 10μM R07 exp344  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp344_vs_exp90.png?nolink |}}
</modal>

<modal id="16" size="lg" title="Cryptotanshinone 12μM R05 exp227  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp227_vs_exp90.png?nolink |}}
</modal>

<modal id="17" size="lg" title="Pterostilbene 16μM R02 exp78  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp78_vs_exp90.png?nolink |}}
</modal>

<modal id="18" size="lg" title="Thioridazine 5μM R08 exp530  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp530_vs_exp90.png?nolink |}}
</modal>

<modal id="19" size="lg" title="Monensin 0.3μM R08 exp507  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp507_vs_exp90.png?nolink |}}
</modal>

<modal id="20" size="lg" title="EPZ-5676 30μM R05 exp233  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp233_vs_exp90.png?nolink |}}
</modal>

<modal id="21" size="lg" title="Nicotine 3000μM R08 exp510  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp510_vs_exp90.png?nolink |}}
</modal>

<modal id="22" size="lg" title="Q15 1 to 2μM on day4 R04 exp164  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp164_vs_exp90.png?nolink |}}
</modal>

<modal id="23" size="lg" title="Q15 2.7μM R02 exp79  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp79_vs_exp90.png?nolink |}}
</modal>

<modal id="24" size="lg" title="CCT251545 20μM R07 exp326  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp326_vs_exp90.png?nolink |}}
</modal>

<modal id="25" size="lg" title="Prochlorperazine 5.2μM R02 exp77  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp77_vs_exp90.png?nolink |}}
</modal>

<modal id="26" size="lg" title="Vemurafenib 6.6μM R02 exp89  vs  WYE-354 6μM R02 exp90">
{{:chemogenomics:comparison_plots:exp89_vs_exp90.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|sphinganine metabolic process|614.22|SPTLC1,SPTLC2|
|C-palmitoyltransferase activity|307.11|SPTLC1,SPTLC2|
|GID complex|307.11|MAEA,WDR26|
|regulation of lipophagy|307.11|SPTLC1,SPTLC2|
|positive regulation of lipophagy|307.11|SPTLC1,SPTLC2|
|serine C-palmitoyltransferase activity|307.11|SPTLC1,SPTLC2|
|sphingomyelin biosynthetic process|245.69|SPTLC1,SGMS1,SPTLC2,OSBP|
|CDP-choline pathway|204.74|PCYT1A,CEPT1|
|sphingomyelin metabolic process|136.49|SPTLC1,SGMS1,SPTLC2,OSBP|
|sphingolipid biosynthetic process|24.33|SPTLC1,SGMS1,SPTLC2,OSBP|
|phospholipid biosynthetic process|18.45|SPTLC1,SGMS1,PCYT1A,SPTLC2,OSBP,CEPT1,PI4KB|
|phospholipid metabolic process|12.43|SPTLC1,SGMS1,PCYT1A,SPTLC2,OSBP,CEPT1,PI4KB|
|organophosphate biosynthetic process|8.28|SPTLC1,SGMS1,PCYT1A,SPTLC2,OSBP,CEPT1,PI4KB|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>