NCK1
Gene Information
- Official Symbol: NCK1
- Official Name: NCK adaptor protein 1
- Aliases and Previous Symbols: N/A
- Entrez ID: 4690
- UniProt: P16333
- Interactions: BioGRID
- PubMed articles: Open PubMed
- OMIM: Open OMIM
Function Summary
- Entrez Summary: N/A
- UniProt Summary: Adapter protein which associates with tyrosine- phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors. {ECO:0000269|PubMed:10026169, ECO:0000269|PubMed:16835242, ECO:0000269|PubMed:17803907, ECO:0000269|PubMed:18835251, ECO:0000269|PubMed:23358419, ECO:0000269|PubMed:9430661}.
Pfam Domains GO Terms
Pfam Domains
| SH3 1 |
| SH2 |
| SH3 2 |
GO Terms
| positive regulation of cap-dependent translational initiation |
| regulation of cap-dependent translational initiation |
| eukaryotic initiation factor eIF2 binding |
| regulation of cap-independent translational initiation |
| positive regulation of cap-independent translational initiation |
| positive regulation of translation in response to endoplasmic reticulum stress |
| negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress |
| negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation |
| positive regulation of cytoplasmic translational initiation |
| regulation of cytoplasmic translational initiation |
| negative regulation of PERK-mediated unfolded protein response |
| positive regulation of translation in response to stress |
| regulation of translation in response to endoplasmic reticulum stress |
| signal complex assembly |
| regulation of PERK-mediated unfolded protein response |
| positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway |
| substrate-dependent cell migration, cell extension |
| protein phosphatase type 1 complex |
| positive regulation of cytoplasmic translation |
| negative regulation of endoplasmic reticulum unfolded protein response |
| negative regulation of transcription from RNA polymerase II promoter in response to stress |
| cytoskeletal adaptor activity |
| peptidyl-serine dephosphorylation |
| regulation of translation in response to stress |
| receptor signaling complex adaptor activity |
| regulation of endoplasmic reticulum unfolded protein response |
| negative regulation of peptidyl-serine phosphorylation |
| substrate-dependent cell migration |
| protein kinase inhibitor activity |
| ephrin receptor binding |
| vesicle membrane |
| positive regulation of translational initiation |
| regulation of cytoplasmic translation |
| regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway |
| lamellipodium assembly |
| negative regulation of insulin receptor signaling pathway |
| negative regulation of cellular response to insulin stimulus |
| positive regulation of response to endoplasmic reticulum stress |
| lamellipodium organization |
| negative regulation of response to endoplasmic reticulum stress |
| ribosome |
| SH3/SH2 adaptor activity |
| receptor tyrosine kinase binding |
| positive regulation of intrinsic apoptotic signaling pathway |
| regulation of insulin receptor signaling pathway |
| vascular endothelial growth factor receptor signaling pathway |
| regulation of cellular response to insulin stimulus |
| protein binding, bridging |
| regulation of translational initiation |
| regulation of response to endoplasmic reticulum stress |
| ephrin receptor signaling pathway |
| positive regulation of actin filament polymerization |
| positive regulation of T cell proliferation |
| regulation of transcription from RNA polymerase II promoter in response to stress |
| regulation of DNA-templated transcription in response to stress |
| positive regulation of translation |
| positive regulation of protein polymerization |
| positive regulation of lymphocyte proliferation |
| positive regulation of mononuclear cell proliferation |
| Fc-gamma receptor signaling pathway involved in phagocytosis |
| immune response-regulating cell surface receptor signaling pathway involved in phagocytosis |
| positive regulation of leukocyte proliferation |
| regulation of peptidyl-serine phosphorylation |
| Fc receptor mediated stimulatory signaling pathway |
| Fc-gamma receptor signaling pathway |
| positive regulation of cellular amide metabolic process |
| regulation of T cell proliferation |
| regulation of intrinsic apoptotic signaling pathway |
| regulation of actin filament polymerization |
| cell-cell junction |
| positive regulation of apoptotic signaling pathway |
| T cell receptor signaling pathway |
| regulation of actin polymerization or depolymerization |
| regulation of actin filament length |
| positive regulation of supramolecular fiber organization |
| positive regulation of T cell activation |
| regulation of lymphocyte proliferation |
| regulation of mononuclear cell proliferation |
| protein dephosphorylation |
| positive regulation of leukocyte cell-cell adhesion |
| positive regulation of cytoskeleton organization |
| regulation of leukocyte proliferation |
| regulation of protein polymerization |
| negative regulation of protein kinase activity |
| T cell activation |
| actin filament organization |
| Fc receptor signaling pathway |
| protein domain specific binding |
| negative regulation of kinase activity |
| positive regulation of protein complex assembly |
| positive regulation of cell-cell adhesion |
| response to endoplasmic reticulum stress |
| regulation of actin filament organization |
| negative regulation of transferase activity |
| positive regulation of neuron projection development |
| antigen receptor-mediated signaling pathway |
| regulation of leukocyte cell-cell adhesion |
| dephosphorylation |
| cadherin binding |
| regulation of T cell activation |
| phagocytosis |
| signaling receptor binding |
| regulation of actin cytoskeleton organization |
| regulation of supramolecular fiber organization |
| regulation of translation |
| positive regulation of lymphocyte activation |
| positive regulation of neuron differentiation |
| regulation of cellular component size |
| positive regulation of cell projection organization |
| lymphocyte activation |
| regulation of actin filament-based process |
| regulation of apoptotic signaling pathway |
| regulation of cell-cell adhesion |
| positive regulation of cell adhesion |
| regulation of cellular amide metabolic process |
| positive regulation of leukocyte activation |
| negative regulation of protein phosphorylation |
| positive regulation of cell activation |
| negative regulation of phosphorylation |
| plasma membrane bounded cell projection assembly |
| immune response-activating cell surface receptor signaling pathway |
| regulation of protein complex assembly |
| supramolecular fiber organization |
| cell projection assembly |
| positive regulation of neurogenesis |
| immune response-regulating cell surface receptor signaling pathway |
| actin cytoskeleton organization |
| regulation of neuron projection development |
| transmembrane receptor protein tyrosine kinase signaling pathway |
| regulation of lymphocyte activation |
| regulation of anatomical structure size |
| positive regulation of cellular component biogenesis |
| posttranscriptional regulation of gene expression |
| positive regulation of nervous system development |
| regulation of cytoskeleton organization |
| positive regulation of cell development |
| negative regulation of phosphate metabolic process |
| negative regulation of phosphorus metabolic process |
| immune response-activating signal transduction |
| actin filament-based process |
| negative regulation of protein modification process |
| immune response-regulating signaling pathway |
| regulation of leukocyte activation |
| positive regulation of organelle organization |
| activation of immune response |
| regulation of cell activation |
| positive regulation of apoptotic process |
| positive regulation of programmed cell death |
| regulation of neuron differentiation |
| regulation of cell adhesion |
| regulation of plasma membrane bounded cell projection organization |
| positive regulation of cell death |
| regulation of cell projection organization |
| enzyme linked receptor protein signaling pathway |
| regulation of cellular response to stress |
| negative regulation of catalytic activity |
| regulation of protein kinase activity |
| regulation of neurogenesis |
| cellular protein-containing complex assembly |
| regulation of cell migration |
| negative regulation of transcription by RNA polymerase II |
| positive regulation of immune response |
| regulation of kinase activity |
| regulation of cell motility |
| positive regulation of cell population proliferation |
| regulation of nervous system development |
| leukocyte activation |
| regulation of cell development |
| regulation of cellular component biogenesis |
| cell migration |
| positive regulation of cell differentiation |
| regulation of transferase activity |
| regulation of locomotion |
| negative regulation of cell death |
| regulation of cellular component movement |
| endoplasmic reticulum |
| positive regulation of intracellular signal transduction |
| negative regulation of cellular protein metabolic process |
| cell activation |
| localization of cell |
| cell motility |
| immune effector process |
| negative regulation of protein metabolic process |
| cytoskeleton organization |
| plasma membrane bounded cell projection organization |
| negative regulation of molecular function |
| regulation of immune response |
| positive regulation of immune system process |
| cell projection organization |
| negative regulation of transcription, DNA-templated |
| positive regulation of cellular component organization |
| positive regulation of transcription by RNA polymerase II |
| negative regulation of nucleic acid-templated transcription |
| negative regulation of RNA biosynthetic process |
| negative regulation of signal transduction |
| regulation of organelle organization |
| response to other organism |
| response to external biotic stimulus |
| locomotion |
| response to biotic stimulus |
| negative regulation of RNA metabolic process |
| negative regulation of cell communication |
| negative regulation of signaling |
| positive regulation of developmental process |
| negative regulation of cellular macromolecule biosynthetic process |
| negative regulation of nucleobase-containing compound metabolic process |
| regulation of protein phosphorylation |
| negative regulation of macromolecule biosynthetic process |
| regulation of response to stress |
| negative regulation of cellular biosynthetic process |
| generation of neurons |
| regulation of apoptotic process |
| positive regulation of transcription, DNA-templated |
| negative regulation of biosynthetic process |
| movement of cell or subcellular component |
| protein-containing complex assembly |
| regulation of programmed cell death |
| regulation of phosphorylation |
| positive regulation of cellular protein metabolic process |
| regulation of cell population proliferation |
| negative regulation of response to stimulus |
| neurogenesis |
| positive regulation of nucleic acid-templated transcription |
| positive regulation of RNA biosynthetic process |
| positive regulation of signal transduction |
| regulation of immune system process |
| regulation of cell death |
| cellular response to stress |
| positive regulation of protein metabolic process |
| negative regulation of gene expression |
| positive regulation of RNA metabolic process |
| positive regulation of multicellular organismal process |
| regulation of phosphate metabolic process |
| regulation of phosphorus metabolic process |
| regulation of cell differentiation |
| positive regulation of cell communication |
| positive regulation of signaling |
| regulation of intracellular signal transduction |
| regulation of protein modification process |
| protein-containing complex subunit organization |
| positive regulation of nucleobase-containing compound metabolic process |
| positive regulation of macromolecule biosynthetic process |
| vesicle-mediated transport |
| positive regulation of cellular biosynthetic process |
| positive regulation of gene expression |
| positive regulation of biosynthetic process |
CRISPR Data
Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Compound Hit
| Screen | Score |
|---|---|
| Milciclib 2μM R08 exp502 | -2.25 |
| Dorsomorphin 5μM R07 exp357 | -2.21 |
| Calcium Ionophore 0.4μM R07 exp342 | -1.92 |
| LLY-284 2.6μM R06 exp291 | -1.81 |
| SNS-032 15μM R08 exp443 | -1.79 |
| Mubritinib 0.2μM R01 exp48 | 1.81 |
| Rapamycin 10μM R00 exp30 | 2.48 |
| Rapamycin 1μM R00 exp29 | 2.71 |
Tissues where Essential in the Avana Dataset (DepMap 20Q1)
Global Fraction of Cell Lines Where Essential: 0/739
| Tissue | Fraction Of Cell Lines Where Essential |
|---|---|
| 1290807.0 | 0/1 |
| 909776.0 | 0/1 |
| bile duct | 0/28 |
| blood | 0/28 |
| bone | 0/26 |
| breast | 0/33 |
| central nervous system | 0/56 |
| cervix | 0/4 |
| colorectal | 0/17 |
| esophagus | 0/13 |
| fibroblast | 0/1 |
| gastric | 0/16 |
| kidney | 0/21 |
| liver | 0/20 |
| lung | 0/75 |
| lymphocyte | 0/16 |
| ovary | 0/26 |
| pancreas | 0/24 |
| peripheral nervous system | 0/16 |
| plasma cell | 0/15 |
| prostate | 0/1 |
| skin | 0/24 |
| soft tissue | 0/9 |
| thyroid | 0/2 |
| upper aerodigestive | 0/22 |
| urinary tract | 0/29 |
| uterus | 0/5 |
Essentiality in NALM6
- Essentiality Rank: 10174
- Expression level (log2 read counts): 4.43
Expression Distribution
NCK1 Expression in NALM6 Cells: 4.43
